The shared NDM-positive strains in the hospital and connecting aquatic environments

Zou, Huiyun; Han, Jingyi; Zhao, Ling; Wang, Di; Guan, Yanyu; Wu, Tianle; Hou, Xinjiao; Han, Hui; Li, Xuewen

doi:10.1016/j.scitotenv.2022.160404

Cited by 5 publications

(3 citation statements)

References 64 publications

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“…Amino glycosides, fluoroquinolones, anti-microbial peptides, rifampin, tetracyclins and elfamycins [30]. Further, bacteria from wastewater and sediments from river bodies also showed ARGs in African countries such as Congo and in India [31,32]. The cheaper and most reliable methods that can be employed is by using QPCR to look for ARGs in low-cost settings.…”

Section: Environmental Factors and Human Activities Resulting In Hgt ...mentioning

confidence: 99%

Pharmaceuticals and Personal Care Products in the Environment with Emphasis on Horizontal Transfer of Antibiotic Resistance Genes

Prasad

Elchuri

2022

Chemistry-Didactics-Ecology-Metrology

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Pharmaceuticals and personal care products (PPCPs) discharged into environment has several adverse impacts. PPCPs are widely utilised for veterinary as well as cosmetic and personal health reasons. These are members of the expanding class of substances known as Contaminants of Emerging Concern (CECs). Antibiotic resistance in the environment and garbage generated by PPCP endanger life. The World Health Organisation (WHO) now recognises antibiotic resistance as a significant global health problem due to the expected increase in mortality caused by it. In the past ten years, mounting data has led experts to believe that the environment has a significant impact on the development of resistance. For human diseases, the external environment serves as a source of resistance genes. It also serves as a major pathway for the spread of resistant bacteria among various habitats and human populations. Large-scale DNA sequencing methods are employed in this thesis to better comprehend the dangers posed by environmental antibiotic resistance. The quantification of the number is an important step in this process. Metagenomic measurement of the number of antibiotic resistance genes in various contexts is a crucial step in this process. However, it’s also crucial to put this data into a broader context by integrating things like taxonomic information, antibiotic concentrations, and the genomic locations of found resistance genes.

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Section: Environmental Factors and Human Activities Resulting In Hgt ...mentioning

confidence: 99%

Pharmaceuticals and Personal Care Products in the Environment with Emphasis on Horizontal Transfer of Antibiotic Resistance Genes

Prasad

Elchuri

2022

Chemistry-Didactics-Ecology-Metrology

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“…The preservation of the bla NDM-1 -containing cluster was probably favored by the presence of last-line antimicrobials like ceftazidime/avibactam and the versatility of this carbapenemase-encoding gene to incorporate with different types of conjugative plasmid backbones. Indeed, the spread of bla NDM-1 through IncFIA plasmids is well documented in the literature [16][17][18][19].…”

Section: Discussionmentioning

confidence: 98%

Probable Three-Species In Vivo Transfer of blaNDM-1 in a Single Patient in Greece: Occurrence of NDM-1-Producing Klebsiella pneumoniae, Proteus mirabilis, and Morganella morganii

et al. 2023

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NDM carbapenemase-encoding genes disseminate commonly among Enterobacterales through transferable plasmids carrying additional resistance determinants. Apart from the intra-species dissemination, the inter-species exchange of plasmids seems to play an additional important role in the spread of blaNDM. We here present the genetics related to the isolation of three species (Klebsiella pneumoniae, Proteus mirabilis, and Morganella morganii) harboring the blaNDM-1 gene from a single patient in Greece. Bacterial identification and antimicrobial susceptibility testing were performed using the Vitek2. Whole genome sequencing and bioinformatic tools were used to identify resistance genes and plasmids. BlaNDM-1 harboring plasmids were found in all three isolates. Moreover, the plasmid constructs of the respective incomplete or circular contigs showed that the blaNDM-1 and its neighboring genes form a cluster that was found in all isolates. Our microbiological findings, together with the patient’s history, suggest the in vivo transfer of the blaNDM-1-containing cluster through three different species in a single patient.

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“…According to the whole-genome sequencing analysis of the pNDM-5 plasmids, the comparison of genetic context flanking bla NDM-5 in both isolates was mostly identical to published plasmids, i.e., IS Aba125 -IS 5 - bla NDM-5 - trpF -IS 26 - umuD -IS Kox3 . The bla NDM-5 genetic structure is widespread in Enterobacteriaceae for bla NDM horizontal transfer and has been reported in bla NDM-5 and bla NDM-9 transmission [ 22 ]. Characterization of the bla NDM-5 genetic contents revealed that it was flanked by multi-insertional sequences.…”

Section: Discussionmentioning

confidence: 99%

Complete-genome sequencing and comparative genomic characterization of blaNDM-5 carrying Citrobacter freundii isolates from a patient with multiple infections

Ye,

Jin,

et al. 2023

BMC Genomics

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Background The emergence and wide spread of carbapenemase-producing Enterobacteriaceae (CPE) poses a growing threat to global public health. However, clinically derived carbapenemase-producing Citrobacter causing multiple infections has rarely been investigated. Here we first report the isolation and comparative genomics of two blaNDM-5 carrying Citrobacter freundii (C. freundii) isolates from a patient with bloodstream and urinary tract infections. Results Antimicrobial susceptibility testing showed that both blaNDM-5 carrying C. freundii isolates were multidrug-resistant. Positive modified carbapenem inactivation method (mCIM) and EDTA-carbapenem inactivation method (eCIM) results suggested metallo-carbapenemase production. PCR and sequencing confirmed that both metallo-carbapenemase producers were blaNDM-5 positive. Genotyping and comparative genomics analyses revealed that both isolates exhibited a high level of genetic similarity. Plasmid analysis confirmed that the blaNDM-5 resistance gene is located on IncX3 plasmid with a length of 46,161 bp, and could successfully be transferred to the recipient Escherichia coli EC600 strain. A conserved structure sequence (ISAba125-IS5-blaNDM-5-trpF-IS26-umuD-ISKox3) was found in the upstream and downstream of the blaNDM-5 gene. Conclusions The data presented in this study showed that the conjugative blaNDM-5 plasmid possesses a certain ability to horizontal transfer. The dissemination of NDM-5-producing C. freundii isolates should be of close concern in future clinical surveillance. To our knowledge, this is the first study to characterize C. freundii strains carrying the blaNDM-5 gene from one single patient with multiple infections.

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The shared NDM-positive strains in the hospital and connecting aquatic environments

Cited by 5 publications

References 64 publications

Pharmaceuticals and Personal Care Products in the Environment with Emphasis on Horizontal Transfer of Antibiotic Resistance Genes

Pharmaceuticals and Personal Care Products in the Environment with Emphasis on Horizontal Transfer of Antibiotic Resistance Genes

Probable Three-Species In Vivo Transfer of blaNDM-1 in a Single Patient in Greece: Occurrence of NDM-1-Producing Klebsiella pneumoniae, Proteus mirabilis, and Morganella morganii

Complete-genome sequencing and comparative genomic characterization of blaNDM-5 carrying Citrobacter freundii isolates from a patient with multiple infections

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