To investigate the sensitivity and significance of different morphological characteristics of plant root systems on vertical pullout resistance, this study considered four main influencing factors: the number of lateral roots, taproot length, the branching angle of the lateral root, and the unit weight of the soil around the root. PC plastic model roots were employed to conduct a vertical pullout orthogonal experiment. A comprehensive μX theoretical analysis method based on the whole root system pullout test was applied for a stress analysis on root segments. Based on the results, the factors affected the vertical pullout resistance of plant root systems in the order of number of lateral roots > taproot length > unit weight of soil around the root > branching angle of the lateral root. When the number of lateral roots increased from 2 to 3, the vertical pullout resistance increased by 64%. Also, when the taproot length increased from 50 to 60 cm, the vertical pullout resistance increased by up to 46%. Furthermore, the unit weight of soil around the roots had a positive linear correlation with vertical pullout resistance. Based on the results, the number of lateral roots and the taproot length were the primary factors affecting the magnitude of the root system’s vertical pullout resistance. When selecting plants for slope protection, plant types with a larger number of lateral roots and longer taproots should be considered as the two most significant factors for achieving a better slope protection methodology.