2010
DOI: 10.1002/pro.384
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The short‐chain oxidoreductase Q9HYA2 from Pseudomonas aeruginosa PAO1 contains an atypical catalytic center

Abstract: The characteristic oxidation or reduction reaction mechanisms of short-chain oxidoreductase (SCOR) enzymes involve a highly conserved Asp-Ser-Tyr-Lys catalytic tetrad. The SCOR enzyme Q9HYA2 from the pathogenic bacterium Pseudomonas aeruginosa was recognized to possess an atypical catalytic tetrad composed of Lys118-Ser146-Thr159-Arg163. Orthologs of Q9HYA2 containing the unusual catalytic tetrad along with conserved substrate and cofactor recognition residues were identified in 27 additional species, the majo… Show more

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“…In recent years, some atypical active sites have been identified in SDRs that lose their enzyme activity but play a role in RNA metabolism and transcriptional regulation (Link et al, 2012 ). For example, MxxxK in NmrA, PglF and CapE (Miyafusa et al, 2013 ; Riegert et al, 2017 ; Stammers et al, 2001 ), LxxxK in HCF244 (Link et al, 2012 ), TxxxR in Q9HYA2 (Huether et al, 2010 ), VxxxK in ROQH1 (Amstutz et al, 2020 ), and CSP41 and SDRvv, which lack the catalytic tetrad (Baker et al, 1998 ; Buysschaert et al, 2013 ). In PglF, MxxxK does not act as a catalytic base like YxxxK; it is Thr 395 and Asp 396 that function together to remove the proton (Riegert et al, 2017 ).…”
Section: Discussionmentioning
confidence: 99%
“…In recent years, some atypical active sites have been identified in SDRs that lose their enzyme activity but play a role in RNA metabolism and transcriptional regulation (Link et al, 2012 ). For example, MxxxK in NmrA, PglF and CapE (Miyafusa et al, 2013 ; Riegert et al, 2017 ; Stammers et al, 2001 ), LxxxK in HCF244 (Link et al, 2012 ), TxxxR in Q9HYA2 (Huether et al, 2010 ), VxxxK in ROQH1 (Amstutz et al, 2020 ), and CSP41 and SDRvv, which lack the catalytic tetrad (Baker et al, 1998 ; Buysschaert et al, 2013 ). In PglF, MxxxK does not act as a catalytic base like YxxxK; it is Thr 395 and Asp 396 that function together to remove the proton (Riegert et al, 2017 ).…”
Section: Discussionmentioning
confidence: 99%