The short neuropeptide F regulates appetitive but not aversive responsiveness in a social insect

Bestea, Louise; Paoli, Marco; Arrufat, Patrick; Ronsin, Brice; Carcaud, Julie; Sandoz, Jean‐Christophe; Velarde, Rodrigo; Giurfa, Martín; Sanchez, María Gabriela de Brito

doi:10.1016/j.isci.2021.103619

Cited by 25 publications

(15 citation statements)

References 94 publications

(135 reference statements)

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“…Whereas the two control groups (sham and DMF) showed similarly low responsiveness scores (mean rank comparison, not significant), the highest dose of both dopamine and 6,7-ADTN significantly increased sucrose scores ( P < 0.0001). We confirmed this finding in bees availing themselves of an even higher energy supplement as they were individually fed with a mixture of honey, pollen, and sucrose solution ( 24 – 26 ) before the sucrose-responsiveness assay. Enhancing dopamine levels in these bees rescued their appetitive responsiveness (fig.…”

supporting

confidence: 70%

Food wanting is mediated by transient activation of dopaminergic signaling in the honey bee brain

Huang

Zhang

Feng

et al. 2022

Science

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The biological bases of wanting have been characterized in mammals, but whether an equivalent wanting system exists in insects remains unknown. In this study, we focused on honey bees, which perform intensive foraging activities to satisfy colony needs, and sought to determine whether foragers leave the hive driven by specific expectations about reward and whether they recollect these expectations during their waggle dances. We monitored foraging and dance behavior and simultaneously quantified and interfered with biogenic amine signaling in the bee brain. We show that a dopamine-dependent wanting system is activated transiently in the bee brain by increased appetite and individual recollection of profitable food sources, both en route to the goal and during waggle dances. Our results show that insects share with mammals common neural mechanisms for encoding wanting of stimuli with positive hedonic value.

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supporting

confidence: 70%

Food wanting is mediated by transient activation of dopaminergic signaling in the honey bee brain

Huang

Zhang

Feng

et al. 2022

Science

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“…Note that this procedure has an intrinsic variability that depends on the amount of Fura-2 delivered by the experimenter and on the number of labeled projection neurons. Still, multiple studies have shown convergent results on odor-elicited glomerular responses, indicating that such a procedure remains valid to investigate olfactory processing in the honey bee antennal lobe (Hourcade et al, 2009; Locatelli et al, 2013; Paoli et al, 2016a; Bestea et al, 2022).…”

Section: Methodsmentioning

confidence: 99%

“…Still, multiple studies have shown convergent results on odor-elicited glomerular responses, indicating that such a procedure remains valid to investigate olfactory processing in the honey bee antennal lobe (Hourcade et al, 2009;Locatelli et al, 2013;Paoli et al, 2016a;Bestea et al, 2022).…”

Section: Bee Preparationmentioning

confidence: 99%

Network plasticity in the honey bee antennal lobe during odor presentation

Paoli

Antonacci

Faes

et al. 2022

Preprint

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Odorant processing presents multiple parallels across animal species, and insects became relevant models for the study of olfactory coding because of the tractability of the underlying neural circuits. Within the insect brain, odorants are received by olfactory sensory neurons and processed by the antennal lobe network. Such a network comprises multiple nodes, named glomeruli, that receive sensory information and are interconnected by local interneurons participating in shaping the neural representation of an odorant. The study of functional connectivity between the nodes of a sensory network in vivo is a challenging task that requires simultaneous recording from multiple nodes at high temporal resolutions. Here, we used fast two-photon microscopy to follow the calcium dynamics of antennal lobe glomeruli and applied Granger causality analysis to assess the functional directed connectivity among network nodes during and after olfactory stimulation. Our findings show that there is a significant causal coupling between network nodes also in the absence of olfactory stimulation. However, connectivity patterns change upon odorant arrival, showing an increase in connection density and conveying odorant-specific information. Furthermore, we show that causal functional connectivity is unique in individual subjects, and cannot be detected upon artificial glomerular scrambling across individuals. This demonstrates that network states do not consist solely of correlations of mean glomerular responses, which are known to be conserved across bees.

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“…bumble bees were kept for an hour under these conditions. For each individual, we measured its consumption (μL) of sucrose solution, quinine or distilled water at the end of this period (Desmedt et al, 2016;Bestea et al, 2022).…”

Section: Experiments 1: Appetitive and Aversive Reinforcement Responsesmentioning

confidence: 99%

Efficient visual learning by bumble bees in virtual‐reality conditions: Size does not matter

et al. 2023

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Recent developments allowed establishing virtual‐reality (VR) setups to study multiple aspects of visual learning in honey bees under controlled experimental conditions. Here, we adopted a VR environment to investigate the visual learning in the buff‐tailed bumble bee Bombus terrestris. Based on responses to appetitive and aversive reinforcements used for conditioning, we show that bumble bees had the proper appetitive motivation to engage in the VR experiments and that they learned efficiently elemental color discriminations. In doing so, they reduced the latency to make a choice, increased the proportion of direct paths toward the virtual stimuli and walked faster toward them. Performance in a short‐term retention test showed that bumble bees chose and fixated longer on the correct stimulus in the absence of reinforcement. Body size and weight, although variable across individuals, did not affect cognitive performances and had a mild impact on motor performances. Overall, we show that bumble bees are suitable experimental subjects for experiments on visual learning under VR conditions, which opens important perspectives for invasive studies on the neural and molecular bases of such learning given the robustness of these insects and the accessibility of their brain.

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The short neuropeptide F regulates appetitive but not aversive responsiveness in a social insect

Cited by 25 publications

References 94 publications

Food wanting is mediated by transient activation of dopaminergic signaling in the honey bee brain

Food wanting is mediated by transient activation of dopaminergic signaling in the honey bee brain

Network plasticity in the honey bee antennal lobe during odor presentation

Efficient visual learning by bumble bees in virtual‐reality conditions: Size does not matter

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