Background: In recent years, single-port laparoscopic colectomy (SPLC) has been introduced as a new technique for colorectal surgery. Despite several studies, available results are too sparse to accurately identify differences between SPLC and conventional multiport laparoscopic colectomy (MPLC). In a continuing effort, the aim of this study was to compare these two techniques in colon cancer patients. Methods: 76 patients with colon cancer who underwent either SPLC or MPLC took part in this study. Demographic data and complications such as age, gender, operative time, hospital stay, intra-operative blood volume loss, and internal organ injury were assessed. The patients were followed up for 6 months. Statistical analysis was performed by SPSS software using Student's t-test, Chi-square test, or Fisher's exact test. Results: The complications were similar in both SPLC and MPLC groups. Operative time was significantly lower in SPLC (P = 0.003). No significant differences were observed in other factors (P > 0.05). Conclusions: According to studies conducted so far, it seems that the difference in the surgeon's experience, types of the colorectal diseases, and types of the colorectal resection may affect the outcomes like operative time and hospital stay. Therefore, further coherent investigations with larger datasets are essential for a detailed comparison.