The significance of cytological examination on reperfusion in rat small intestinal transplantation

Abstract

We examined the cytology of the exudate in preserved intestinal grafts on reperfusion and compared it with the histological findings in rat small intestinal transplantation. The jejunal graft was harvested from the Lewis rat and was preserved in University of Wisconsin solution for 6, 12, 24 and 48 h at 4 degrees C (n = 6, in each group) and was then syngeneically transplanted. On reperfusion, the exudate was collected and studied cytologically. Full thickness biopsies were performed at the end of the preservation and at 30 min after reperfusion for histological examination. Histological examination after reperfusion showed that the crypt layer was preserved until 24 h. However, it was destroyed by 48 h preservation. The cytological findings correlated with the depth of tissue injury shown histologically. The degeneration of villus epithelial cells, the decrease in the content of mucin in both the goblet cells as well as villus cells, and the appearance of crypt cells are all considered to be signs of poor graft viability. Cytological examination is therefore recommended as an effective, non-invasive and real-time method for evaluating graft viability just after reperfusion in small intestinal transplantation.

Different Ischemic Preconditioning Regimens Affecting Preservation Injury of Intestines

“…Progressive decline in GC due to ischemic injury during cold storage induces a decline in mucus production and loss of its protective effect on the intestinal mucosa [5], leading to invasion of exogenous and endogenous microorganisms and strengthening of bacterial translocation. The decrease in GC population and villus cell mucus content during intestinal graft preservation is considered to be an important sign of poor graft viability [33]. In our study, a rapid decrease in GC populations with advancing time of preservation was noted in all groups, although the progress of GC decline in groups with IPC was less marked.…”

Epidermal Growth Factor Stimulates the Recovery of Glucose Absorption after Small Bowel Transplantation

“…The rats were anesthetized with a mixture of intraperitoneal sodium pentobarbital (40 mg/kg) and atropine sulfate (0.1 mg/kg). The operation was performed in two stages according to the method of Monchik and Russell [5,29], with a minor modification. In the first stage, a 20-cm segment of the jejunum was isolated on a pedicle of the aorta and portal vein using an operative microscope.…”

Preservation Injury of Jejunal Grafts and Its Modulation by Custodiol and University of Wisconsin Perfusion Solutions in Wistar Rats

“…in the preservation solution). In experimental models, other groups have determined serotonin levels in the preservation solution and effluate during ischemia [2,12], or the presence of serotonin-positive cells in the bowel wall during rejection [20]. Our study was designed to determine serotonin levels in specimens of the small bowel mucosa of rats.…”