The significance of E. coli treatment in perinatal period

Ljubić, Aleksandar; Nikolić, Ljubinka I.; Stefanović, Slobodanka B.; Popović, Zoran V.; Bojanic, Nebojsa; Anojcić, Petar; Marković, Srđan Z.; Mijajlović, Miloš S.; Kastratović, Dragana A.

doi:10.5937/hpimj1602416l

Cited by 1 publication

(1 citation statement)

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“…However, depending on the nature and dose of radiation, cells do respond to ionizing radiation. Platelets are anuclear cellular fragments generated from megakaryocytes and are the primary regulators in the hemostasis and thrombosis [4]. Platelet products are routinely irradiated to avoid medical complications associated with platelet transfusion [5].…”

Section: Introductionmentioning

confidence: 99%

X-rays Stimulate Granular Secretions and Activate Protein Kinase C Signaling in Human Platelets

Khan

Liu

Meng

et al. 2023

CIMB

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X-rays can induce morphological as well as functional changes in cells. Platelets are anuclear cellular fragments originating from megakaryocytes and are the major regulators in hemostasis and thrombosis. Platelet products are irradiated to avoid medical complications associated with platelet transfusion. So far, gamma, UV, and laser radiation have been used for this purpose. However, scientists are divided about the effects of radiation on platelet quality. The present study was designed to explore the possible effects of X-rays in washed human platelets and understand the molecular mechanism behind them. In the present study, we exposed washed human platelets to 10 or 30 Gy X-rays at 0.25 Gy/min. Flow cytometry, aggregometry, and western blot were performed to investigate the effect of X-rays on platelet degranulation, integrin activation, platelet aggregation, and apoptosis. It was found that X-rays immediately induced granular secretions with no effect on GP IIb/IIIa activation. Not surprisingly, due to granule secretions in irradiated platelets, platelet aggregation was significantly reduced. In contrast to granular secretions and platelet aggregation, X-rays induced mitochondrial transmembrane potential depolarization in a time-dependent manner to induce apoptosis and activated protein kinase C (PKC) signaling. This study revealed and explained the molecular mechanism activated by X-rays in washed human platelets. Here we also introduced Gö 6983, a PKC inhibitor, as an agent that counteracts X-ray-induced changes and maintains the integrity of platelets.

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Section: Introductionmentioning

confidence: 99%