The Significance of Expression Pattern of Dab1 and Reelin in the Postnatal Bladder of Dab1 -/- (Yotari) Mice

Abstract

Background. The aim of our study is to provide an insight into the genetic expression landscape of Dab1 and Reelin, which are important in human genitourinary tract development and could help elucidate the critical stages of the onset of bladder anomalies.Methods. Yotari, heterozygote and wild type mice were sacrificed on the 4th, 11th and 14th postnatal day. Morphological parameters were analyzed using immunohistochemistry on mice bladder samples.Results. Significant expression of Dab1 was observed in the bladder epithelium during the 4th postnatal day and in the wt -wild type, but also in the yot -/- mouse. Reelin expression was significant in bladder epithelium during the 4th postnatal day in the wild-type and mutant mouse type. A significant expression of Dab1 positive cells in lamina propria was observed on the 4th postnatal day in yot -/- and in wt. The percentage of Reelin positive cells in lamina propria was significant on the 4th postnatal day in mutated and wild-type mice.Conclusions. An increase in the expression of Dab1 and Reelin proteins in the bladder of a yotari mouse may indicate bladder damage, due to congenital renal abnormalities and caused by a mutation in the Dab1 gene.Key words: Dab1; Reelin, CAKUT, bladder development, yotari mice