The significance of structure in certain enzymic processes

Oparin, A.I.; Gel’man, Nina S.; Deborin, G.A.

doi:10.1016/0003-9861(57)90522-2

Cited by 9 publications

(6 citation statements)

References 4 publications

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“…We have no explanation to offer for this phenomenon. 120 In conclusion these results indicate that ergosterol does not activate trypsin either for synthetic or protein substrates under conditions, described earlier in the literature [3]. The only explanation we can offer to account for the difference between the present and earlier results is that in the present work/3-trypsin has been used, whereas the trypsin used earlier was less well defined.…”

Section: Resultssupporting

confidence: 44%

“…The results of the above study have been discussed recently by others [4]. Since the observation of a direct influence of a sterol on the catalytic activity of trypsin would be of interest for a better understanding of the mechanism of action both of trypsin and of steroid hormones, we wish to report briefly here on the results of an attempt to reproduce the published study on trypsin activation by ergosterol [3]using a pure enzyme sample (~-trypsin) and more modern methods for following the degree of hydrolysis of substrate.…”

Section: Introductionmentioning

confidence: 80%

“…On the other hand, according to earlier work trypsin forms a stable complex with ergosterol and the enzymic activity of this complex against serum albumin and egg albumin as substrates was higher than the activity of trypsin alone [3] indicating that the ligand interacts directly with the enzyme. The results of the above study have been discussed recently by others [4].…”

Section: Introductionmentioning

confidence: 83%

“…For the formation of a complex between the enzyme and ergosterol the procedure orginally described [3] was adopted. A freshly prepared enzyme solution (3.5 X 10 -5 M/~-trypsin in 1.t0-3N HCI or in 0.06 M phosphate buffer, pH 8.5) was mixed in a mortar with an excess of crystalline ergosterol (10 mg/ml solution) for 10 min at room temperature and the resulting suspension was faltered.…”

Section: Methodsmentioning

confidence: 99%

“…A freshly prepared enzyme solution (3.5 X 10 -5 M/~-trypsin in 1.t0-3N HCI or in 0.06 M phosphate buffer, pH 8.5) was mixed in a mortar with an excess of crystalline ergosterol (10 mg/ml solution) for 10 min at room temperature and the resulting suspension was faltered. Also the experimental conditions described originally for the digestion of egg albumin by trypsin at different enzyme-substrate ratios [3] were followed.…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Does ergosterol influence tryptic activity?

Zylber

Keil

1973

FEBS Letters

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Section: Resultssupporting

confidence: 44%

Section: Introductionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 83%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Does ergosterol influence tryptic activity?

Zylber

Keil

1973

FEBS Letters

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Some Aspects of Enzyme Reactions in Heterogeneous Systems

Packer

1970

Advances in Enzymology - And Related Areas of Molecular Biology

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Double Emulsion Droplets as a Plausible Step to Fatty Acid Protocells

Douliez

2023

Small Methods

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It is assumed that life originated on the Earth from vesicles made of fatty acids. These amphiphiles are the simplest chemicals, which can be present in the prebiotic soup, capable of self‐assembling into compartments mimicking modern cells. Production of fatty acid vesicles is widely studied, as their growing and division. However, how prebiotic chemicals require to further yield living cells encapsulated within protocells remains unclear. Here, one suggests a scenario based on recent studies, which shows that phospholipid vesicles can form from double emulsions affording facile encapsulation of cargos. In these works, water‐in‐oil‐in‐water droplets are produced by microfluidics, having dispersed lipids in the oil. Dewetting of the oil droplet leaves the internal aqueous droplet covered by a lipid bilayer, entrapping cargos. In this review, formation of fatty acid protocells is briefly reviewed, together with the procedure for preparing double emulsions and vesicles from double emulsion and finally, it is proposed that double emulsion droplets formed in the deep ocean where undersea volcano expulsed materials, with fatty acids (under their carboxylic form) and alkanols as the oily phase, entrapping hydrosoluble prebiotic chemicals in a double emulsion droplet core. Once formed, double emulsion droplets can move up to the surface, where an increase of pH, variation of pressure and/or temperature may have allowed dewetting of the oily droplet, leaving a fatty acid vesicular protocell with encapsulated prebiotic materials.

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The significance of structure in certain enzymic processes

Cited by 9 publications

References 4 publications

Does ergosterol influence tryptic activity?

Does ergosterol influence tryptic activity?

Some Aspects of Enzyme Reactions in Heterogeneous Systems

Double Emulsion Droplets as a Plausible Step to Fatty Acid Protocells

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