The Significance of the “One-Minute” (Prompt Direct Reacting) Bilirubin in Serum 1

A Chromatographic Method for the Determination of the Three Bile Pigments in Serum

“…Regression line y=0.64x+0.24 is superimposed. minute bilirubin" present was more closely related to the total amount of direct-reacting pigment than to a particular pigment, a finding that agrees with that of Klatskin and Drill (1950).…”

Uptake and release of bilirubin by skin

“…Free bilirubin is relatively insoluble in aqueous medium (Burnstine & Schmid, 1962) and like other weakly acidic substances is transported by serum bound to the proteins (Klatskin & Bungards, 1950;Ostrow & Schmid, 1963;Cooke & Robert, 1969). The relative distribution of bilirubin in various tissues would depend therefore on the bilirubin binding capacity of serum constituents.…”

Bilirubin-Protein Linkages in Serum and Their Relationship to the Van Den Bergh Reaction 1

“…The diazotization curves obtained in this experiment are illustrated in Figure 5. It will be observed that in all three solutions less than 10 per cent of the bilirubin reacted directly during the first minute, and less than 15 per cent in 30 minutes, behavior characteristic of indirect-reacting serum (14). When alcohol was added, there was a prompt increase in the rate of diazotization in all tubes.…”

“…Blood was drawn from patients with various types of jaundice and allowed to coagulate at room temperature. Following centrifugation the supernatant serum was analyzed for its content of directreacting ("one-minute") and total bilirubin by a modification (14) of the Malloy-Evelyn method (15 One strip in each chamber was streaked with 0.10 ml. of serum and was used for the separation and identification of the pigment in each of the protein fractions.…”

“…In particular it made it possible to characterize the properties of the pigments isolated from each of the protein fractions, and to compare them with those present in the original serum. Due consideration was given to the fact that all sera contain both direct-and indirect-reacting bilirubin, and that the qualitative van den Bergh reaction, used to identify the pigments in many similar investigations, merely distinguishes between low and high concentrations of direct-reacting pigment (1,14).…”