The significance of the SDF-1/CXCR4 signaling pathway in the normal development

Age-related liver endothelial zonation triggers steatohepatitis by inactivating pericentral endothelium-derived C-kit

“…We may preliminarily draw a conclusion that pericentral liver EC-derived C-kit decelerates NASH progression, however periportal EC-derived Notch accelerates NASH, which partially testify our hypothesis that LSECs from different zonation of liver contribute distinctly to NASH pathogenesis. CXCR4/SDF-1 signaling involved in chemokine signaling pathways extensively modulates the mobilization and recruitment of varieties of immune cells [35][36][37][38] . In this study, we found that the expression of CXCR4 increased in both the mRNA and protein levels in LSECs lacking C-kit.…”

LASP1 in Cellular Signaling and Gene Expression: More than Just a Cytoskeletal Regulator

“…Chemokine signaling is essential for coordinated cell migration in health and disease. Typically, chemokines signal through heptahelical, G protein-coupled receptors [ 19 ]. LASP1 interacts with the LKIL motif (aa 327–330) at the intracellular C-terminus of the chemokine receptors CXCR2 and CXCR4 which are overexpressed in breast cancer [ 20 ].…”

Towards personalized antibody cancer therapy: development of a microfluidic cell culture device for antibody selection

“…While in microfluidics cells were grown inside the microfluidic device under continuous conditions allowing their growth in adherent conditions, the cells used for flow cytometry were detached from the T-flask, with the assays carried out under suspension conditions. Given that the internalization of CXCR4 influences many downstream biological processes, like migration, transcriptional activation or adhesion, 69 the fact that cells were adhered or in suspension or even static in a well or under continuous conditions might influence the outcome results. A possible experiment for future studies could be the monitoring of downstream pathway analytes to shed light on how the assay conditions influence pathway activation upon CXCR4 internalization.…”