The Simultaneous Human FVIII/vWF Purification and Virus Inactivation Combined in Chromatographic Column

Volkov, Georgii L

doi:10.4172/2167-7956.1000157

J Biomol Res Ther

2017

DOI: 10.4172/2167-7956.1000157

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The Simultaneous Human FVIII/vWF Purification and Virus Inactivation Combined in Chromatographic Column

Georgii L Volkov¹

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2022

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High Affinity Peptides in Processes of IgG Purification, Chromatographic Column Virus Inactivation/Elimination and Titer of Anti-Rubella IgG Enrichment

Havryliuk¹,

Krasnobryzha²,

Havryliuk³

et al. 2022

J Biomed Res Environ Sci

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According to "The Proteome Code" concept introduced by J. Biro and our early development of affinity peptide calculation method it was studied the possibility of high affinity peptide chromatographic gels development for IgG1-4 separation from the donor plasma. Given the next step of virus inactivation of IgG directly in the chromatographic column, the affinity gel had bind IgG at several spatially spaced points in order to limit the degree of freedom of the protein for retention IgG at high buffer flow rate or elevated buffer temperatures without denaturation. In addition, the possibility of creating highly specific affinity sense-antisense peptides against Rubella virus in order to increase the titer of aRIgG in plasma or even its isolation in highly purified form was studied. Based on previous experiments, an affinity multi-peptide chromatographic gel with the following properties was developed: the DBC with enough residence time 10 min was around 50-54 mg × mL-1 of total 98.0% purity of IgG with natural proportion of the 1-4 subclasses, any other immunoglobulins were not found. The virus inactivation/elimination on this gel directly in chromatographic column shown a highly effective virus elimination (log10>9) for both nonenveloped and lipid enveloped viruses. Using RV sequence from UniProt_KB and dates from more than 20 literature sources on the virus proteins interaction, affinity peptides were calculated against virus proteins C and E1,2. Then these peptides were modified to reach more affinity enhancement and affinity-peptide chromatographic gel was synthetized. By this gel from total mass IgG1-4 contained 6644 IU anti-Rubella IgG with specificity 6.64 IU × mg-1 were isolated 5382 IU aRIgG (> 80%) with a specificity of 791 IU × mg-1.

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High Affinity Peptides in Processes of IgG Purification, Chromatographic Column Virus Inactivation/Elimination and Titer of Anti-Rubella IgG Enrichment

Havryliuk¹,

Krasnobryzha²,

Havryliuk³

et al. 2022

J Biomed Res Environ Sci

View full text Add to dashboard Cite

show abstract

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The Simultaneous Human FVIII/vWF Purification and Virus Inactivation Combined in Chromatographic Column

Cited by 1 publication

References 31 publications

High Affinity Peptides in Processes of IgG Purification, Chromatographic Column Virus Inactivation/Elimination and Titer of Anti-Rubella IgG Enrichment

High Affinity Peptides in Processes of IgG Purification, Chromatographic Column Virus Inactivation/Elimination and Titer of Anti-Rubella IgG Enrichment

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