The Simultaneous Voltammetric Determination of Aflatoxins В₁ and М₁ on a Glassy-Carbon Electrode

Abstract

For the first time, the possibility of using stripping voltammetry for the simultaneous determination of aflatoxins В1 and М1 on a glassy-carbon electrode has been shown. The influence of various factors (Еэ, τэ, w, and the nature of the background electrolyte) on the potential and magnitude of the oxidation current of mycotoxins has been estimated. Working conditions for voltammetric determination and reproducibility of analytical signals for two mycotoxins have been selected. The mutual influence of aflatoxins В1 and М1 on the value of analytical signals in their simultaneous presence has been studied. It has been found that, in the range of their detectable contents, the presence of aflatoxin В1 reduces the analytical signal of aflatoxin М1 by 45–50%, but the linearity of the calibration dependence is preserved. The content of aflatoxin М1 in determination of aflatoxin B1 does not exert a significant effect in the range of 10–15%. Based on the results obtained, a procedure has been proposed for determining the content of aflatoxins В1 and М1 in their joint presence in milk by voltammetry in the concentration ranges 2 × 10−3 ÷ 2 × 10−1 mg/dm3 and 2 × 10−4 ÷ 2 × 10−2 mg/dm3, respectively (Sr not more than 18%).

Voltammetric determination of Aflatoxin B1 using a bismuth layer-gold nanoparticles modified glassy carbon electrode

“…Commercial milk samples were analyzed considering the pretreatment reported by Slepchenko et al [31] with modi cations. 25 mL of milk are mixed with 1 mL of 6 M hydrochloric acid, then stirred for 15 minutes, and centrifuged at 15,000 rpm within 15 minutes.…”

A Novel Voltammetric Approach For The Quantification of Aflatoxin B1 Using a Bismuth-Modified Electrode

“…42 Repeatability, reproducibility, validation of the proposed methodology and AFB1 determination in milk.-Milk samples were analyzed according to the procedure described by Slepchenko, et al with some modifications. 43 A sample of 25 ml of milk is mixed with 1 ml of concentrated hydrochloric acid (approximately 6 M). The mixture is slightly stirred, left for 15 min, poured into centrifuge tubes, and centrifuged at 15,000 rpm within 15 min 5 g of ammonium sulfate is added to the centrifugate until the salt dissolves.…”

Assessment of Poultry Feed Contamination Level by Aflatoxin B1: Quantification by Two Chromatographic Analysis Methods

“…Its structure is represented in Figure 1. In natural conditions, aflatoxin B1 contaminates cereals, legumes, various nuts, oil seeds, cocoa and coffee, animal feed as well as other food products [21] [22].…”