The single-cell sequencing: new developments and medical applications

Tang, Xiaoning; Huang, Yongmei; Lei, Jinli; Luo, Hui; Zhu, Xiao

doi:10.1186/s13578-019-0314-y

Cited by 254 publications

(174 citation statements)

References 61 publications

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“…On the one hand, autophagy can keep the genome stable by removing damaged organelles and misfolded proteins, so it can inhibit the growth of cancerous cells [46]. On the other hand, autophagy provides the tumor with more nutrients, which strengthens the tumor's ability to cope with extreme environments [47,48].…”

Section: Autophagy and Apoptosis Of Cancermentioning

confidence: 99%

mTOR signaling pathway and mTOR inhibitors in cancer: progress and challenges

et al. 2020

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Mammalian target of rapamycin (mTOR) regulates cell proliferation, autophagy, and apoptosis by participating in multiple signaling pathways in the body. Studies have shown that the mTOR signaling pathway is also associated with cancer, arthritis, insulin resistance, osteoporosis, and other diseases. The mTOR signaling pathway, which is often activated in tumors, not only regulates gene transcription and protein synthesis to regulate cell proliferation and immune cell differentiation but also plays an important role in tumor metabolism. Therefore, the mTOR signaling pathway is a hot target in anti-tumor therapy research. In recent years, a variety of newly discovered mTOR inhibitors have entered clinical studies, and a variety of drugs have been proven to have high activity in combination with mTOR inhibitors. The purpose of this review is to introduce the role of mTOR signaling pathway on apoptosis, autophagy, growth, and metabolism of tumor cells, and to introduce the research progress of mTOR inhibitors in the tumor field.

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Section: Autophagy and Apoptosis Of Cancermentioning

confidence: 99%

mTOR signaling pathway and mTOR inhibitors in cancer: progress and challenges

et al. 2020

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“…For example, in the above slip mechanism, when the mutated MS is paired with another chain, the redundant structure that may be formed after the slip chain can be restored to the level before replication if it is cut by nuclease and repaired, and remain in the new chain if it is not repaired. MMR deficient (dMMR) makes the errors produced during DNA replication impossible to repair, which leads to nucleotide mutation and changes in the length of simple repeat MS sequence [2,23].…”

Section: Mmr Deficientmentioning

confidence: 99%

Microsatellite instability: a review of what the oncologist should know

et al. 2020

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The patients with high microsatellite instability (MSI-H)/mismatch repair deficient (dMMR) tumors recently have been reported that can benefit from immunotherapy, and MSI can be used as a genetic instability of a tumor detection index. However, many studies have shown that there are many heterogeneous phenomena in patients with MSI tumors in terms of immunotherapy, prognosis and chemotherapy sensitivity. Here we mainly review the research results of MSI detection methods, the mechanisms of MSI occurrence and its relationship with related tumors, aiming to make a brief analysis of the current research status of MSI and provide comparable reference and guidance value for further research in this field.

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“…Soysa et al found that Hand2 led to dysregulation of retinoicacid signaling and disruption of anterior-posterior patterning by using temporal single-cell-transcriptome analysis and in situ hybridization (42). These findings showed that scRNA-seq is critical for understanding pathological mechanisms of congenital heart disease in a single-cell resolution; it is expected that scRNA-seq can be applied to identify various diseases that have been caused by heterogeneous cell differentiation (11).…”

Section: Cell Differentiation and Cell Lineage Analysis In Cardiac Dementioning

confidence: 99%

Single-cell and spatial transcriptomics approaches of cardiovascular development and disease

Roth

Kim

et al. 2020

BMB Rep.

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Recent advancements in the resolution and throughput of single-cell analyses, including single-cell RNA sequencing (scRNA-seq), have achieved significant progress in biomedical research in the last decade. These techniques have been used to understand cellular heterogeneity by identifying many rare and novel cell types and characterizing subpopulations of cells that make up organs and tissues. Analysis across various datasets can elucidate temporal patterning in gene expression and developmental cues and is also employed to examine the response of cells to acute injury, damage, or disruption. Specifically, scRNA-seq and spatially resolved transcriptomics have been used to describe the identity of novel or rare cell subpopulations and transcriptional variations that are related to normal and pathological conditions in mammalian models and human tissues. These applications have critically contributed to advance basic cardiovascular research in the past decade by identifying novel cell types implicated in development and disease. In this review, we describe current scRNA-seq technologies and how current scRNA-seq and spatial transcriptomic (ST) techniques have advanced our understanding of cardiovascular development and disease. [BMB Reports 2020; 53(8): 393-399]

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The single-cell sequencing: new developments and medical applications

Cited by 254 publications

References 61 publications

mTOR signaling pathway and mTOR inhibitors in cancer: progress and challenges

mTOR signaling pathway and mTOR inhibitors in cancer: progress and challenges

Microsatellite instability: a review of what the oncologist should know

Single-cell and spatial transcriptomics approaches of cardiovascular development and disease

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