A milestone in understanding life in the universe is the detection of biosignature gases in the atmospheres of habitable exoplanets. Future mission concepts under study by the 2020 decadal survey, e.g., Habitable Exoplanet Imaging Mission (HabEx) and the Large UV/Optical/IR Surveyor (LUVOIR), have the potential of achieving this goal. We investigate the baseline requirements for detecting four molecular species, H 2 O, O 2 , CH 4 , and CO 2 , assuming concentrations of these species equal to that of modern Earth. These molecules are highly relevant to habitability and life on Earth and other planets. Through numerical simulations, we find the minimum requirements of spectral resolution, starlight suppression, and exposure time for detecting biosignature and habitability marker gases. The results are highly dependent on cloud conditions. A low-cloud case is more favorable because of deeper and denser lines whereas a no-cloud case is the pessimistic case for its low albedo. The minimum exposure time for detecting a certain molecule species can vary by a large factor (∼10) between the low-cloud case and the no-cloud case. For all cases, we provide baseline requirements for HabEx and LUVOIR. The impact of exozodiacal contamination and thermal background is also discussed and will be included in future studies.