The single nucleotide polymorphisms rs11761556 and rs12706832 of the leptin gene are associated with type 2 diabetes mellitus in the Iraqi population

Abstract

This study was conducted to assess the potential association between leptin (LEP) gene polymorphisms and type 2 diabetes mellitus (T2DM) in Iraqi patients. Genomic DNA was extracted from 120 diabetic subjects and 100 controls. Three specific PCR fragments were designed to flank three highly frequent single nucleotide polymorphism (SNP)s within LEP, rs11761556, rs12706832 and rs2167270. The amplified loci were genotyped by PCR-single-strand conformation polymorphism (SSCP) followed by Sanger sequencing for representative genotypes. Logistic regression analysis was performed to detect the association between the targeted genetic variants and T2DM. PCR-SSCP genotyping showed three banding patterns for all three targeted SNPs. Individuals with the AA genotype in both rs11761556 and rs12706832 SNPs showed significantly higher (P<0.05) body mass index (BMI), waist circumference (WC), fasting blood glucose (FBG), hemoglobin A1c (HbA1c), homeostatic model assessment for insulin resistance (HOMA-IR), insulin, low-density lipoprotein cholesterol (LDL-C) and triglyceride (TG) values than those with other genotypes. Association analysis revealed that individuals with the A allele exhibited a greater risk of T2DM. Data of the present investigation indicated that both rs11761556 and rs12706832 SNPs exerted a noticeable association with T2DM. The study suggests implementing both rs11761556 and rs12706832 SNPs in the early detection of T2DM.

[Retracted] Meta‐Analysis of Long‐Term Efficacy of Proliferative Diabetic Retinopathy Based on Intelligent Medical Treatment

“…DAG activates intracytoplasmic protein kinase C, which eventually induces endothelial cell growth, increases vascular permeability, and promotes the formation of new blood vessels. Studies have shown that VEGF can induce the rapid phosphorylation of vascular endothelial cell-specific adhesion proteins Ocladen and Zonula Ocladen-1, so that it can be transferred from the intercellular junction to intracellular, and also increase the production of reactive oxygen species, resulting in increased retinal vascular permeability [ 5 ]. VEGF induces endothelial cells to expression of urokinase and tissue-type plasminogen activator, plasminogen activator, and interstitial collagenase and also leads to abnormal deposition of some extracellular matrix components in vascular walls.…”

Two missense variants of the epidermal growth factor receptor gene are associated with non small cell lung carcinoma in the Iraqi population

“…Denatured PCR products were loaded onto neutral polyacrylamide gels with 8% concentration, and electrophoresis was performed for about 4h at 200 V and 100 mA. The polyacrylamide gels were xed and stained by silver nitrate following the recommended protocol [32]. To validate the detected electrophoretic genotypes, each PCR-SSCP banding pattern was exposed to Sanger sequencing.…”