The SIPIBEL project: treatment of hospital and urban wastewater in a conventional urban wastewater treatment plant

Chonova, Teofana; Lecomte, Vivien; Bertrand-Krajewski, Jean-Luc; Bouchez, Agnès; Labanowski, Jérôme; Dagot, Christophe; Lévi, Yves; Perrodin, Yves; Wiest, Laure; Gonzalez-Ospina, Adriana; Cournoyer, Benoît; Sebastian, Christel

doi:10.1007/s11356-017-9302-0

Cited by 21 publications

(15 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The experimental site is a pilot WWTP handling separately urban and hospital wastewater using biological treatment with conventional activated sludge system (Chonova et al, 2018b) (Figure 1A). The hospital whose effluents flow directly without special pretreatment to the WWTP has 450 beds, and the urban network includes around 20,850 inhabitants.…”

Section: Methodsmentioning

confidence: 99%

Benthic Diatom Communities in an Alpine River Impacted by Waste Water Treatment Effluents as Revealed Using DNA Metabarcoding

et al. 2019

Self Cite

View full text Add to dashboard Cite

Freshwater ecosystems are continuously affected by anthropogenic pressure. One of the main sources of contamination comes from wastewater treatment plant (WWTP) effluents that contain wide range of micro- and macropollutants. Chemical composition, toxicity levels and impact of treated effluents (TEs) on the recipient aquatic ecosystems may strongly differ depending on the wastewater origin. Compared to urban TEs, hospital ones may contain more active pharmaceutical substances. Benthic diatoms are relevant ecological indicators because of their high species and ecological diversity and rapid response to human pressure. They are routinely used for water quality monitoring. However, there is a knowledge gap on diatom communities’ development and behavior in treated wastewater in relation to prevailing micro- and macropollutants. In this study, we aim to (1) investigate the response of diatom communities to urban and hospital TEs, and (2) evaluate TEs effect on communities in the recipient river. Environmental biofilms were colonized in TEs and the recipient river up- and downstream from the WWTP output to study benthic diatoms using DNA metabarcoding combined with high-throughput sequencing (HTS). In parallel, concentrations of nutrients, pharmaceuticals and seasonal conditions were recorded. Diatom metabarcoding showed that benthic communities differed strongly in their diversity and structure depending on the habitat. TE sites were generally dominated by few genera with polysaprobic preferences belonging to the motile guild, while river sites favored diverse communities from oligotrophic and oligosaprobic groups. Seasonal changes were visible to lower extent. To categorize parameters important for diatom changes we performed redundancy analysis which suggested that communities within TE sites were associated to higher concentrations of beta-blockers and non-steroidal anti-inflammatory drugs in urban effluents vs. antibiotics and orthophosphate in hospital effluents. Furthermore, indicator species analysis showed that 27% of OTUs detected in river downstream communities were indicator for urban or hospital TE sites and were absent in the river upstream. Finally, biological diatom index (BDI) calculated to evaluate the ecological status of the recipient river suggested water quality decrease linked to the release of TEs. Thus, in-depth assessment of diatom community composition using DNA metabarcoding is proposed as a promising technique to highlight the disturbing effect of pollutants in Alpine rivers.

show abstract

Section: Methodsmentioning

confidence: 99%

Benthic Diatom Communities in an Alpine River Impacted by Waste Water Treatment Effluents as Revealed Using DNA Metabarcoding

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…The main characteristics of the plant during the sampling period are shown in Table 1 and described in Chonova et al (2017), in this issue. The WWTP is a low-loaded plant, designed for carbon removal and nitrification.…”

Section: Sampling Sitesmentioning

confidence: 99%

“…Four sampling sites were selected: urban influent and effluent, hospital influent and effluent (more details available in Chonova et al (2017) in this special issue). WW were sampled before pre-treatment and treated WW after the secondary treatment.…”

Section: Samplingmentioning

confidence: 99%

Two-year survey of specific hospital wastewater treatment and its impact on pharmaceutical discharges

Wiest

Chonova

Bergé

et al. 2017

Environ Sci Pollut Res

Self Cite

View full text Add to dashboard Cite

It is well known that pharmaceuticals are not completely removed by conventional activated sludge wastewater treatment plants. Hospital effluents are of major concern, as they present high concentrations of pharmaceutically active compounds. Despite this, these specific effluents are usually co-treated with domestic wastewaters. Separate treatment has been recommended. However, there is a lack of information concerning the efficiency of separate hospital wastewater treatment by activated sludge, especially on the removal of pharmaceuticals. In this context, this article presents the results of a 2-year monitoring of conventional parameters, surfactants, gadolinium, and 13 pharmaceuticals on the specific study site SIPIBEL. This site allows the characterization of urban and hospital wastewaters and their separate treatment using the same process. Flow proportional sampling, solid-phase extraction, and liquid chromatography coupled with tandem mass spectrometry were used in order to obtain accurate data and limits of quantification consistent with ultra-trace detection. Thanks to these consolidated data, an in-depth characterization of urban and hospital wastewaters was realized, as well as a comparison of treatment efficiency between both effluents. Higher concentrations of organic carbon, AOX, phosphates, gadolinium, paracetamol, ketoprofen, and antibiotics were observed in hospital wastewaters compared to urban wastewaters. Globally higher removals were observed in the hospital wastewater treatment plant, and some parameters were shown to be of high importance regarding removal efficiencies: hydraulic retention time, redox conditions, and ambient temperature. Eleven pharmaceuticals were still quantified at relevant concentrations in hospital and urban wastewaters after treatment (e.g., up to 1 μg/L for sulfamethoxazole). However, as the urban flow was about 37 times higher than the hospital flow, the hospital contribution appeared relatively low compared to domestic discharges. Thanks to the SIPIBEL site, data obtained from this 2-year program are useful to evaluate the relevance of separate hospital wastewater treatment.

show abstract

“…Three different exposure variables were collected simultaneously: (1) heavy metals anionic, non-ionic and cationic, measured as described by Chonova et al (2018). Microorganisms were recovered from filters after water filtration on 45 µm filters and DNA was isolated using the Power Water DNA Extraction Kit (MoBio Laboratories Inc., Carlsbad, CA, USA).…”

Section: Chemicalsmentioning

confidence: 99%

Modelling the impact of urban and hospital wastewaters eco-exposomes on the antibiotic-resistance dynamics

Henriot

Buelow

Petit

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Antibiotic-resistance emergence and selection have become major public health issues globally. The presence of antibiotic resistant bacteria (ARB) in natural and anthroposophical environments threatens to compromise the sustainability of care in human and animal populations. This study was undertaken to develop a simple model formalizing the selective impact of antibiotics and pollutants on the dynamics of bacterial resistance in water and use the model to analyze longitudinal spatiotemporal data collected in hospital and urban wastewaters. Longitudinal-sampling data were collected between 2012 and 2015 in four different locations in Haute-Savoie, France: hospital and urban wastewaters, before and after water-treatment plants. Concentration in three different types of compounds: 1) heavy metals 2) antibiotics and 3) surfactants; and abundance of 88 individual genes and mobile genetic elements, mostly conferring resistance to antibiotics, were simultaneously collected. A simple hypothesis-driven model describing the weekly ARB dynamics was proposed to fit available data by assuming normalized gene abundance to be proportional to ARB populations in water. Compounds impacts on the dynamics of 17 genes found in multiple sites were estimated. We found that while mercury and vancomycin had relevant effects on ARB dynamics, respectively positively affecting the dynamics of 10 and 12 identified genes, surfactants antagonistically affected genes dynamics (identified for three genes). This simple model enables analyzing the relationship between resistance-gene persistence in aquatic environments and specific compounds inherent to human activities. Applying our model to longitudinal data, we identified compounds that act as co-selectors for antibiotic resistance.HighlightsWe analyzed longitudinal wastewater resistance genes and environmental dataWe developed a simple hypothesis-driven model to assess resistance selectionMercury and vancomycin were key drivers of antibiotic resistance in wastewater

show abstract

The SIPIBEL project: treatment of hospital and urban wastewater in a conventional urban wastewater treatment plant

Cited by 21 publications

References 23 publications

Benthic Diatom Communities in an Alpine River Impacted by Waste Water Treatment Effluents as Revealed Using DNA Metabarcoding

Benthic Diatom Communities in an Alpine River Impacted by Waste Water Treatment Effluents as Revealed Using DNA Metabarcoding

Two-year survey of specific hospital wastewater treatment and its impact on pharmaceutical discharges

Modelling the impact of urban and hospital wastewaters eco-exposomes on the antibiotic-resistance dynamics

Contact Info

Product

Resources

About