The Sizes and Interrelations of Lymphocytes in Thoracic Duct Lymph and Lymph Node of Normal and Stimulated Rats

Rieke, William O.; Everett, N. B.; Caffrey, Ruth W.

doi:10.1159/000208112

Cited by 24 publications

(7 citation statements)

References 4 publications

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“…Similar values were obtained when splenic dendritic cells were exposed to [3H]thymidine in vitro (14). These data confirm that dendritic cells are not large lymphocytes, which label with a much higher frequency in vitro (6) and in vivo (5,11).…”

Section: Resultssupporting

confidence: 82%

Identification of a Novel Cell Type in Peripheral Lymphoid Organs of Mice

Steinman¹,

Cohn²

1974

The Journal of Experimental Medicine

595

349

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Previous studies have indicated that peripheral lymphoid organs contain a small population of an apparently novel cell type (13). These ~cells adhere to glass and plastic surfaces and are called "dendritic" cells, because their most striking morphological feature is the presence of many cell processes of varying size and shape. A number of tests in vitro demonstrated that dendritic cells do not have the functional properties of lymphocytes, macrophages, or nonphagocytic "reticular" cells (14). In this paper, we outline several properties of dendritic cells in situ, features which have been selected because of their demonstrated relevance in understanding the physiology of lymphoid organs. These properties include ontogeny, origin, steroid and radiosensitivity, kinetics and turnover, and response to antigen administration. In addition to their intrinsic importance, these characteristics of dendritic cells in situ further differentiate them from other previously described cell types in peripheral lymphoid organs. A most interesting feature of dendritic cells is that they are not rapidly proliferating but yet are constantly turning over at a surprisingly rapid rate under steady state conditions. The influx of new dendritic cells derives from a proliferating precursor pool located in bone marrow and in the nonglass-adherent cells of spleen. Materials and MethodsM/ce.--Inbred DBA/2, C57BL, and F1 hybrid (B6DFx) mice were purchased from the Jackson Laboratories, Bar Harbor, Maine. Outbred, 8-wk old, germ-free mice were obtained from Charles River Breeding Laboratories, Wilmington, Mass, while specific pathogen-free NCS mice were provided by The Rockefeller University colony.Dendritic Cells--Dendritic cells were harvested from spleen and lymph node suspensions prepared by manually dissociating the organs with fine forceps. Treatment with 0.05% collagenase (wt/vol in medium 199, Fx I, Sigma Chemical Co., St. Louis, Mo.) was used in instances where large numbers of splenic macrophages were required (14). Dendritic ceils were cultivated, identified, and quantitated as previously described (13).Effects of Ionizing Irradiation and Steroids.--Groups of 3-4 mice were examined 2 days * Supported by grants AI 07012 and AI

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Section: Resultssupporting

confidence: 82%

Identification of a Novel Cell Type in Peripheral Lymphoid Organs of Mice

Steinman¹,

Cohn²

1974

The Journal of Experimental Medicine

595

349

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“…In mesenteric lymph node, spieen and Peyer's patches, most of the labeled cells were localized within the germinal centers. The time sequences for the appearance of labeled lymphocytes of different sizes were the same as those with [meth~l-~HIdThd labeling experiments Rieke, Everett & Caffrey, 1963). Two rats were killed for each time.…”

Section: Resultsmentioning

confidence: 99%

Preferential Labeling of Rat Lymphocytes With a Rapid Rate of Turnover by Tritiated Deoxycytidine

Amano

Everett

1976

Cell Proliferation

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Lymphocytes in thymic cortex and germinal centers of lymphoid tissues are labeled intensely with generally labeled tritiated deoxycytidine [G-3H]dCyd whereas they are weakly labeled with methyl tritiated deoxythymidine [methyl-3H]dThd of the same specific activity, not only by single injection but also by an intensive injection schedule. [G-3H]dCyd can be used to label short-lived lymphocytes strongly, although not specifically. The distribution patterns of labeled lymphocytes were different depending on the injection schedules of [G-3H]dCyd.[G-3H]dCyd can be used as a precursor molecule for cytosine and also thymine found in DNA. The ratios of radioactive thymine to cytosine measured biochemically on DNA extracted from radioactive lymphocytes labeled by the various schedules indicate strongly that short-and long-lived lymphocyte populations have different abilities to utilize pyrimidine nucleosides for DNA synthesis.

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“…Problems here involve possible rcutilization of the label and limitation of the observation to the particular cell compart ment studied. Because of the great change in cell size and morphology, attempts have been made to quantitate growth by accounting for the change in cell diameter or surface area of cell populations [16,[35][36][37]. Since cell mass is equivalent to volume (r3) and not surface area (r2) or diameter (2r) it seems wiser to quantitate cell growth by net volume change.…”

Section: Discussionmentioning

confidence: 99%