The skin blanching assay with halcinonide, influence of halcinonide concentration and application time ⋆

Abstract

Objective Instrumental colour readings (Minolta Chromameter CR200) were performed in order to quantify corticosteroid induced skin blanching.Metbods Different Halcinonide concentrations in the same vehicle ranging from 0.005% to 0.200% were tested on the volar part of the forearms. Besides dose-response relationships we studied the influence of application time by applying the formulations under occlusion during 2, 12 and 18 h.Results All evaluated formulations provoked a discernible blanching as indicated by the significant increase of the L' parameter and the decrease of the a' parameter. Discrimination between the different Halcinonide concentrations was possible only in the 2 h application experiment. With longer application times a similar blanching was recorded for all Halcinonide concentrations.Conclusion Our results indicate that a shorter application time may lead to a more sensitive skin blanching assay. Keywords: Skin blanching; Corticosteroid concentration and application time 0926-9959/95/$09.50 © 1995 Elsevier Science B.V. All rights reserved SSDI 0926-9959(95)00103-4 p.

Skin color measurements: comparison between three instruments: the Chromameter®, the DermaSpectrometer® and the Mexameter®

“…Furthermore, various skin color changes induced under different dermato-cosmetic treatments, such as erythema, irritation, blanching and tanning, were considered and quantitatively evaluated by the three instruments. Erythema was induced by the vasodilatator methylnicotinate (7,21), irritation by exposure to sodium lauryl sulfate (3), skin blanching by the corticosteroid halcinonide (16,(22)(23)(24), artificial tanning by using dihydroxyacetone (25,26) on the volar part of both forearms and natural UV tanning on the face. These variations in skin color were compared quantitatively by the three instruments.…”

Influence of the timing of ultrasound application on the penetration of corticosteroids

“…Ultrasound was applied using the Sonopuls 992 ultrasound transducer (Enraf Nonius, Delft, the Netherlands). A commercially available corticosteroid formulation, Halcinonide 1% (Betacorton ® , Spirig, Egerkingen, Switzerland) was used as indicator molecule . Skin surface color was recorded using the Minolta Chroma Meter CR 200 operating in the CIE L * a * b * color space (CIELAB).…”