The sll1951 Gene Encodes the Surface Layer Protein of Synechocystis sp. Strain PCC 6803

Trautner, Christoph; Vermaas, Wim F. J.

doi:10.1128/jb.00615-13

Cited by 50 publications

(57 citation statements)

References 51 publications

(55 reference statements)

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“…). Sll1951 is the main constituent of the S‐layer (Trautner and Vermaas, ) and was recently identified as a substrate of the T1SS in Synechocystis (Oliveira et al ., ). In order to further investigate the presence/absence of the S‐layer in the various Synechocystis strains, two additional approaches were taken.…”

Section: Resultssupporting

confidence: 69%

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Identification of inner membrane translocase components of TolC‐mediated secretion in the cyanobacterium Synechocystis sp. PCC 6803

Gonçalves

Pacheco

Tamagnini

et al. 2018

Environmental Microbiology

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Cyanobacteria were the first organisms ever to perform oxygenic photosynthesis and still significantly contribute to primary production on a global scale. To assure the proper functioning of their primary metabolism and cell homeostasis, cyanobacteria must rely on efficient transport systems to cross their multilayered cell envelope. However, cyanobacterial secretion mechanisms remain largely unknown. Here, we report on the identification of 11 putative inner membrane translocase components of TolC-mediated secretion in the unicellular cyanobacterium Synechocystis sp. PCC 6803. Gene-inactivation of each of the candidate genes followed by a comprehensive phenotypic characterization allowed to link specific protein components to the processes of protein export (as part of the type I secretion system) and drug efflux (part of the resistance-division-nodulation efflux pumps). In addition, mutants in genes sll0141, sll0180 and slr0369 exhibited alterations in pilin glycosylation, but pili structures could still be observed by transmission electron microscopy. By studying the release of outer membrane vesicles (OMVs), an alternative secretion route, on mutants with impaired secretory functions we suggest that the hyper-vesiculating phenotype of the TolC-deficient mutant is related to cell envelope stress management. Altogether, these findings highlight how both classical (TolC-mediated) and nonclassical (OMVs-mediated) secretion systems are crucial for cyanobacterial cell homeostasis.

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Section: Resultssupporting

confidence: 69%

“…). While in the Synechocystis wild‐type strain it was possible to recognize a reticulated lattice as the outermost cell wall structure, consistent with the described S‐layer (Trautner and Vermaas, ), the mutant strains Δ tolC , Δ sll0141 , Δ sll0180 , Δ sll1180 and Δ sll1181 lacked such structure.…”

Section: Resultsmentioning

confidence: 97%

Identification of inner membrane translocase components of TolC‐mediated secretion in the cyanobacterium Synechocystis sp. PCC 6803

Gonçalves

Pacheco

Tamagnini

et al. 2018

Environmental Microbiology

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“…1A). The S-layer protein of Synechocystis was identified as a hemolysin-like protein encoded by the sll1951 gene (Sakiyama et al 2006; Trautner and Vermaas 2013). …”

Section: Discussionmentioning

confidence: 99%

Lipid and carotenoid cooperation-driven adaptation to light and temperature stress in Synechocystis sp. PCC6803

Zakar

Herman

Vajravel

et al. 2017

Biochimica et Biophysica Acta (BBA) - Bioenergetics

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Polyunsaturated lipids are important components of photosynthetic membranes. Xanthophylls are the main photoprotective agents, can assist in protection against light stress, and are crucial in the recovery from photoinhibition. We generated the xanthophyll- and polyunsaturated lipid-deficient ROAD mutant of Synechocystis sp. PCC6803 (Synechocystis) in order to study the little-known cooperative effects of lipids and carotenoids (Cars). Electron microscopic investigations confirmed that in the absence of xanthophylls the S-layer of the cellular envelope is missing. In wild-type (WT) cells, as well as the xanthophyll-less (RO), polyunsaturated lipid-less (AD), and the newly constructed ROAD mutants the lipid and Car compositions were determined by MS and HPLC, respectively. We found that, relative to the WT, the lipid composition of the mutants was remodeled and the Car content changed accordingly. In the mutants the ratio of non-bilayer-forming (NBL) to bilayer-forming (BL) lipids were found considerably lower. Xanthophyll to β-carotene ratio increased in the AD mutant. In vitro and in vivo methods demonstrated that saturated, monounsaturated lipids and xanthophylls may stabilize the trimerization of Photosystem I (PSI). Fluorescence induction and oxygen-evolving activity measurements revealed increased light sensitivity of RO cells compared to those of the WT. ROAD showed a robust increase in light susceptibility and reduced recovery capability, especially at moderate low (ML) and moderate high (MH) temperatures, indicating a cooperative effect of xanthophylls and polyunsaturated lipids. We suggest that both lipid unsaturation and xanthophylls are required for providing the proper structure and functioning of the membrane environment that protects against light and temperature stress.

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“…The hemolysin-like protein Sll1951, which localizes in the S-layer of Synechocystis 6803, is important for the tolerance to heavy metals and osmotic stress, suggesting that it has a general role in combating these stressors (Sakiyama et al 2006(Sakiyama et al , 2011Trautner and Vermaas 2013). The adapted strains had an insertion mutation in sll1951 at 715 nt; thus, the adapted strains encoded a Sll1951 protein which was shorter than that of the P strain.…”

Section: Whole-genome Sequencing Analysis Of the Adapted Strainsmentioning

confidence: 95%

Genomic analysis of parallel-evolved cyanobacterium Synechocystis sp. PCC 6803 under acid stress

et al. 2015

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Experimental evolution is a powerful tool for clarifying phenotypic and genotypic changes responsible for adaptive evolution. In this study, we isolated acid-adapted Synechocystis sp. PCC 6803 (Synechocystis 6803) strains to identify genes involved in acid tolerance. Synechocystis 6803 is rarely found in habitants with pH < 5.75. The parent (P) strain was cultured in BG-11 at pH 6.0. We gradually lowered the pH of the medium from pH 6.0 to pH 5.5 over 3 months. Our adapted cells could grow in acid stress conditions at pH 5.5, whereas the parent cells could not. We performed whole-genome sequencing and compared the acid-adapted and P strains, thereby identifying 11 SNPs in the acid-adapted strains, including in Fo F1-ATPase. To determine whether the SNP genes responded to acid stress, we examined gene expression in the adapted strains using quantitative reverse-transcription polymerase chain reaction. sll0914, sll1496, sll0528, and sll1144 expressions increased under acid stress in the P strain, whereas sll0162, sll0163, slr0623, and slr0529 expressions decreased. There were no differences in the SNP genes expression levels between the P strain and two adapted strains, except for sll0528. These results suggest that SNPs in certain genes are involved in acid stress tolerance in Synechocystis 6803.

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The sll1951 Gene Encodes the Surface Layer Protein of Synechocystis sp. Strain PCC 6803

Cited by 50 publications

References 51 publications

Identification of inner membrane translocase components of TolC‐mediated secretion in the cyanobacterium Synechocystis sp. PCC 6803

Identification of inner membrane translocase components of TolC‐mediated secretion in the cyanobacterium Synechocystis sp. PCC 6803

Lipid and carotenoid cooperation-driven adaptation to light and temperature stress in Synechocystis sp. PCC6803

Genomic analysis of parallel-evolved cyanobacterium Synechocystis sp. PCC 6803 under acid stress

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