The sluggish-A gene of Drosophila melanogaster is expressed in the nervous system and encodes proline oxidase, a mitochondrial enzyme involved in glutamate biosynthesis.

Abstract

Certain gene mutations in Drosophila melanogaster cause sluggish motor activity. We have localized the

Improved functional abilities of the life-extended Drosophila mutant Methuselah are reversed at old age to below control levels

“…The distance walked in a fixed period of time has previously been used as a measure of exploratory behavior and locomotor ability in Drosophila (Strauss et al 1992;Hayward et al 1993;Roberts 1998;Petrosyan et al 2007). The current experiment measured average walking distance for both genotypes in a large well-lit arena (60×45 cm).…”

An essential cell division gene of Drosophila , absent from Saccharomyces , encodes an unusual protein with tubulin-like and myosin-like peptide motifs

“…Four parts of the misato region (denoted transforming fragments, A, B, C, and D; see Fig. 1) were cloned into the vector pW8 by previously described procedures (12)(13)(14)(15) and used for transgenic analysis. Construct A consists of a 10.9-kb BamHI genomic fragment; construct B is a 4.8-kb SpeI-PstI fragment; construct C is an 11.6-kb BglII fragment; and construct D is a 15.3-kb EcoRI-XhoI genomic fragment used previously to rescue the sluggish gene.…”

Data transferability from model organisms to human beings: Insights from the functional genomics of the flightless region of Drosophila

“…DNA and RNA extractions, blotting and hybridization, screening, and cloning of cDNAs from gt10 and gt11 Drosophila libraries were performed as previously described (2,(4)(5)(6)(7)(8). mRNA preparations from dissected tissues of a white 1 stock were performed by using TRIzol and the conditions recommended by the supplier (GIBCO͞BRL).…”

“…We first characterized a substantial part of the fly X chromosome bracketed by the genes amnesiac and suppressor of forked ( Fig. 1), obtained an overview of the mutational properties and phenotypes resulting from perturbations of this 2-megabase region (1), then focused on a 67-kb subregion that has been the ongoing focus of our laboratories (2)(3)(4)(5)(6)(7)(8)(9) and that is anchored by the flightless locus. We now present the genomic and cDNA sequences for this region, the phenotypic analyses at the cellular and organismal levels after genetic, transgenic, and deficiency perturbations, and the evolutionary genomics.…”

“…Chromosomal breakpoints were mapped as previously described (6), and 8 parts of the fly genomic region (denoted T1 to T8 in Fig. 1) were cloned into the vector pW8 and used in constructing transgenic organisms (2)(3)(4)8). …”