The smooth muscle cell in culture

Chamley-Campbell, J.; Campbell, Gordon; Ross, Russell

doi:10.1152/physrev.1979.59.1.1

Cited by 1,445 publications

(779 citation statements)

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“…Primary cultures of rat aortic SMC were isolated from SpragueDawley rats (Chamley-Campbell et al, 1979). In brief, the tunica media was minced and digested in 5 ml of the digestion solution (2 mg/ml collagenase, 0.125 mg/ml elastase, 0.25 mg/ml soybean trypsin inhibitor, 2.0 mg/ml crystallized bovine albumin) for 45 min at 37°C.…”

Section: Vsmc Culturementioning

confidence: 99%

CCN4 Regulates Vascular Smooth Muscle Cell Migration and Proliferation

Liu

Dong

Lin

et al. 2013

Molecules and Cells

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Section: Vsmc Culturementioning

confidence: 99%

CCN4 Regulates Vascular Smooth Muscle Cell Migration and Proliferation

Liu

Dong

Lin

et al. 2013

Molecules and Cells

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“…The ability of isolated or cultured smooth muscle cells to contract or relax in response to specific agonists is well documented [30,31] and airway smooth muscle cells are no exception. AVNER et al [32] reported that subconfluent primary cultures of canine tracheal smooth muscle cells contracted in response to the cholinomimetic, carbachol.…”

Section: Heterogeneity and Phenotypic Modulation Of Airway Smooth Musclementioning

confidence: 99%

“…Nevertheless, there is increasing acceptance that, in primary culture, exposure of airway smooth muscle cells to a mitogenic stimulus (e.g. serum) results in a reversible modulation in phenotype from contractile to synthetic [30,37] that is characterized by the loss and subsequent re-expression of specific contractile phenotype markers [38,39], as outlined in figure 1 and table 1. Contractile cells are characterized by a high density of contractile proteins and few biosynthetic intracellular organelles, are mitotically quiescent and retain their ability to contract in response to spasmogens.…”

Section: Heterogeneity and Phenotypic Modulation Of Airway Smooth Musclementioning

confidence: 99%

“…Contractile cells are characterized by a high density of contractile proteins and few biosynthetic intracellular organelles, are mitotically quiescent and retain their ability to contract in response to spasmogens. Synthetic smooth muscle cells have a low density of contractile proteins and high fraction of biosynthetic organelles, are mitotically active and may lose their ability to contract [30,37]. It is probably incorrect, however, to consider these states as mutually exclusive.…”

Section: Heterogeneity and Phenotypic Modulation Of Airway Smooth Musclementioning

confidence: 99%

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Phenotypic diversity and molecular mechanisms of airway smooth muscle proliferation in asthma

Hirst¹,

Walker²,

Chilvers³

2000

Eur Respir J

109

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Chronic persistent asthma is characterized by poorly reversible airflow obstruction and airways inflammation and remodelling. Histopathological studies of airways removed at post mortem from patients with severe asthma reveal marked inflammatory and architectural changes associated with airway wall thickening. Increased airway smooth muscle content, occurring as a result of hyperplastic and/or hypertrophic growth, is believed to be one of the principal contributors to airway wall thickening. In recent years, significant advances have been made in elucidating the mediators and the intracellular pathways that regulate proliferation of airway smooth muscle. The contribution that smooth muscle makes to persistent airflow obstruction may not, however, be limited simply to its increased bulk within the airway wall. Interest is growing in the possibility that reversible phenotypic modulation and increased heterogeneity of airway smooth muscle function may also be a feature of the asthmatic airway. This review focuses on possible mechanisms controlling smooth muscle phenotype heterogeneity as well as on the mediators and intracellular pathways implicated in its cellular proliferation. Particular attention is paid to mechanisms involving activation of the extracellular signal regulated kinase‐, protein kinase C‐ and phosphoinositide 3‐kinase‐dependent pathways, since these appear to be the major candidate second messenger pathways for G protein‐ and tyrosine kinase‐coupled receptor‐stimulated proliferation.

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“…Contractile SMCs are elongated, spindleshaped cells, whereas synthetic SMCs are less elongated and have a cobblestone morphology which is referred to as epithelioid or rhomboid (figure 1). 3,4 Synthetic SMCs contain a high number of organelles involved in protein synthesis, whereas these are largely replaced by contractile filaments in contractile SMCs. Moreover, synthetic and contractile SMCs have different proliferative and migratory characteristics.…”

mentioning

confidence: 99%

Regulation and characteristics of vascular smooth muscle cell phenotypic diversity

Rensen¹,

Doevendans²,

Eys³

2007

NHJL

776

706

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Vascular smooth muscle cells can perform both contractile and synthetic functions, which are associated with and characterised by changes in morphology, proliferation and migration rates, and the expression of different marker proteins. The resulting phenotypic diversity of smooth muscle cells appears to be a function of innate genetic programmes and environmental cues, which include biochemical factors, extracellular matrix components, and physical factors such as stretch and shear stress. Because of the diversity among smooth muscle cells, blood vessels attain the flexibility that is necessary to perform efficiently under different physiological and pathological conditions. In this review, we discuss recent literature demonstrating the extent and nature of smooth muscle cell diversity in the vascular wall and address the factors that affect smooth muscle cell phenotype. (Neth Heart J 2007;15:100-8.)

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The smooth muscle cell in culture

Cited by 1,445 publications

References 0 publications

CCN4 Regulates Vascular Smooth Muscle Cell Migration and Proliferation

CCN4 Regulates Vascular Smooth Muscle Cell Migration and Proliferation

Phenotypic diversity and molecular mechanisms of airway smooth muscle proliferation in asthma

Regulation and characteristics of vascular smooth muscle cell phenotypic diversity

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