Parents may, directly and indirectly, influence their children in developing prosocial behaviour. However, it is uncertain to what extent and how blood donor behaviour is transmitted from one generation to the next. We used linked data from the first wave of the Donor InSight Survey and the Dutch blood donor registry (n=23,037) to examine intergenerational transmission of blood-donor behaviour among blood donors in the Netherlands. We found that 38% of the Dutch donor population is part of a blood donor family (i.e., at least two generations of donors in the family). Additionally, our preregistered analyses revealed that three mechanisms are relevant for the transmission of donor behaviour from the parent to the child generation, namely parental blood-donor identity, the number of donations parents made, and the developmental period of the child during which parents donated. The outcomes of our study may be used by blood banks in designing their donor recruitment strategies towards families.