There is a scarcity of research exploring gender biases in student-teacher interactions, especially within virtual simulations. This chapter addresses this research gap by delving into teacher-student interactions within the virtual teaching simulator (VTS). Simulations play a pivotal role in aiding pre-service teachers. Given the historical marginalization of girls/women in mathematics, the analysis focuses on uncovering gender biases in teacher-students interactions in virtual math lessons. The findings indicate relatively equitable participation of girls in VTS, albeit with occasional disparities; notably, the authors observed a lack of girls' engagement in high-cognitive-demand talk.