The Solanum pimpinellifolium Cf-ECP1 and Cf-ECP4 genes for resistance to Cladosporium fulvum are located at the Milky Way locus on the short arm of chromosome 1

Soumpourou, Eleni; Iakovidis, Michail; Chartrain, Laëtitia; Lyall, Verity; Thomas, Colwyn M.

doi:10.1007/s00122-007-0638-6

Cited by 21 publications

(22 citation statements)

References 30 publications

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“…It is composed of polymers of galacturonic acid residues that are a-d-(1,4)-linked at the O1 and O4 positions and sometimes methylated at C6 and/or acetylated at O2 and/or O3 positions (Lerouxel, Cavalier, Liepman, & Keegstra, 2006;Soumpourou, Iakovidis, Chartrain, Lyall, & Thomas, 2007). Polygalacturonases catalyze the hydrolysis of glycosidic a-1-4 linkages in pectic acid.…”

Section: Introductionmentioning

confidence: 99%

Biochemical characterization of three distinct polygalacturonases from Neosartorya fischeri P1

Pan

et al. 2015

Food Chemistry

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Section: Introductionmentioning

confidence: 99%

Biochemical characterization of three distinct polygalacturonases from Neosartorya fischeri P1

Pan

et al. 2015

Food Chemistry

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“…It is an environmentally benign method that can be used as an alternative to chemicals, as their applicability Cf-9B [167] Cf-ECP1 S. pimpinellifolium [173] Cf-ECP2 S. pimpinellifolium [174] Cf-ECP4 S. pimpinellifolium [173] Cf-ECP5 [175] Cf-ECP6 [176] Cf-ECP7 [176] Cf Ph-2 S. pimpinellifolium [186] Ph-3 S. pimpinellifolium [187] ph-4 [188] ph-5 M.R. Foolad et al, unpublished data Prf Pseudomonas syringae pv tomato [20] Pto S. pimpinellifolium [189] Py-1 Pyrenochaeta lycopersici S. peruvianum [190] Pot-1 Potato virus Y, Tobacco etch virus S. habrochaites [191] Sm Stemphyllium solani S. Pimpinelifolium [192] [193] Sw-6 Tomato spotted wilt virus S. peruvianum [194] Sw-7 S. chilense [195] Ty-1 Tomato yellow leaf curl virus S. chilense [196] Ty-2 S. habrochaites [197] Ty-3…”

Section: Tomato Resistance Genes and Their Modes Of Actionmentioning

confidence: 99%

Resistance-Gene-Mediated Defense Responses against Biotic Stresses in the Crop Model Plant Tomato

Chaudhary¹,

Hs²

2017

J Plant Pathol Microbiol

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Complex series of defense response activation, consistent with the studies conducted in the model plant Arabidopsis thaliana, has been demonstrated in tomato during incompatible pathogen/pest interactions. During the past two decades, numerous tomato genes have been identified conferring resistance to diverse pathogens/ pests in a gene-for-gene manner. A few of these cloned resistance (R)-genes (Cf and Pto) have been extensively studied and excellent existing reviews describe R-gene function, interacting proteins and the mechanism of Avirulence effector perception. Recent comprehensive gene expression analysis of tomato responses to biotic stresses resulted in identification of genes and potential molecular processes that are associated with several of the tomato R-genemediated resistance responses. The purpose of this review is to provide an overview of tomato R-gene-mediated defense responses to different pathogens/pests together with the components involved in the organization of this highly complex network of plant defenses. Global transcriptome profiling is an important initial step for dissecting biological systems particularly with systems where not much is known about the molecular basis of the resistance response. The enrichment of the tomato EST databases initiated several genomewide profiling studies [32][33][34][35]. This facilitated significant progress in the characterization of tomato incompatible responses to Avr pathogens, contributing to future gene identification and to the understanding of the potential molecular processes that are associated with the different tomato R-gene-mediated resistances [33]. With the advent of next generation sequencing technologies, and the tomato genome sequence [36], additional genome wide studies have been conducted. With a genome sequence and a high-density linkage and molecular maps [37], combined with being a host for numerous pathogens and pests, tomato has emerged as a powerful model system for crop plant defense response studies. Moreover, the introgression of R-genes from wild species into cultivated tomato provides a unique opportunity to study different resistance mechanisms against very diverse biotic agents in a single plant. In this review, we summarize the current understanding of R-genes in tomato and the downstream signaling components that are critical for activating defense responses. In addition, we discuss the current and future technologies that will significantly enhance our knowledge about tomato-pathogen interactions and will provide alternative strategies to develop a sustainable resistance. Tomato Resistance Genes and their Modes of ActionHost resistance is an important component of a sustainable disease management system [38]. It is an environmentally benign method that can be used as an alternative to chemicals, as their applicability against Verticillium. The Ve locus contains two closely linked and inversely oriented genes, Ve1 and Ve2, both encoding a RLP-type cell surface receptor. Ve1 R-gene provides resistance against ra...

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“…To date, these genes have been mapped to just two chromosomal segments in the tomato genome. The Milky Way (MW) locus on the short arm of chromosome 1, and several genetically linked loci (like ORION; OR) containing functional Cf genes, encode a large number of genes with distinct recognition specificities including Cf-4, Hcr9-4E, Cf-9, Hcr9-9B, Cf-19, Cf-Ecp1, Cf-Ecp2, Cf-Ecp3, Cf-Ecp4, Cf-Ecp5 (JONES et al 1994;THOMAS et al 1997;PARNISKE et al 1999;TAKKEN et al 1999;HAANSTRA et al 2000;PANTER et al 2002;KRUIJT et al 2004;SOUMPOUROU et al 2007;ZHAO et al 2016). These genes were designated as Hcr9s (homologs of Cladosporium resistance gene 9).…”

Section: Introductionmentioning

confidence: 99%

“…DNA preparation and marker sequence analysis. DNA was prepared from individual tomato lines and bulked segregant pools as described previously (THOMAS et al 1995;SOUMPOUROU et al 2007). AFLP analysis of tomato genomic DNA was performed as described previously (THOMAS et al 1995).…”

Section: Introductionmentioning

confidence: 99%

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Genes encoding recognition of theCladosporium fulvumeffector protein Ecp5 are encoded at several loci in the tomato genome

Iakovidis

Soumpourou

Anderson

et al. 2019

Preprint

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The molecular interactions between tomato and Cladosporium fulvum have been an important model for molecular plant pathology. Complex genetic loci on tomato chromosomes 1 and 6 harbor genes for resistance to Cladosporium fulvum, encoding receptor like-proteins that perceive distinct Cladosporium fulvum effectors and trigger plant defenses. Here, we report classical mapping strategies for loci in tomato accessions that respond to Cladosporium fulvum effector Ecp5, which is very sequencemonomorphic. We screened 139 wild tomato accessions for an Ecp5-induced hypersensitive response, and in five accessions, the Ecp5-induced hypersensitive response segregated as a monogenic trait, mapping to distinct loci in the tomato genome. We identified at least three loci on chromosomes 1, 7 and 12 that harbor distinct Cf-Ecp5 genes in four different accessions. Our mapping showed that the Cf-Ecp5 in Solanum pimpinellifolium G1.1161 is located at the Milky Way locus. The Cf-Ecp5 in Solanum pimpinellifolium LA0722 was mapped to the bottom arm of chromosome 7, while the Cf-Ecp5 genes in Solanum lycopersicum Ontario 7522 and Solanum pimpinellifolium LA2852 were mapped to the same locus on the top arm of chromosome 12. Bi-parental crosses between accessions carrying distinct Cf-Ecp5 genes revealed putative genetically unlinked suppressors of the Ecp5-induced hypersensitive response. Our mapping also showed that Cf-11 is located on chromosome 11, close to the Cf-3 locus. The Ecp5-induced hypersensitive response is widely distributed within tomato species and is variable in strength. This novel example of convergent evolution could be used for choosing different functional Cf-Ecp5 genes according to individual plant breeding needs.Cf-Ecp4 or Cf-Ecp5 genes that could be used in future studies. We found that Cf-Ecp5 was distributed in a wider geographical region than Cf-Ecp4. Lastly, we report the chromosomal locations of Cf-11, which maps to the top arm of chromosome 11, close to the previously reported Cf-3 gene (KANWAR et al. 1980). All in all, this study revealed a unique example of multiple convergently evolved tomato loci in different accessions 7 associated with the HR to C. fulvum Ecp5 effector that have not been previously reported in plant-pathogen interactions. MATERIALS AND METHODSPlant and fungal materials. Seeds from core collections of wild and domesticated tomato species were obtained from the C.M. Rick Tomato Genetics Resource Center, University of California, Davis, USA. These species included Solanum arcanum, Solanum cheesmaniae, Solanum chilense, Solanum chmielewskii, Solanum corneliomulleri, Solanum galapagense, Solanum habrochaites, Solanum huaylasense, Solanum lycopersicoides, Solanum lycopersicum, Solanum neorickii, Solanum ochranthum, Solanum pennellii, Solanum peruvianum and Solanum sitiens. Additional stocks containing previously characterised Cf-Ecp genes were obtained from Dr M. H. A. J. Joosten and Dr P. Lindhout (University of Wageningen) including the accessions S. pimpinellifolium LA1683 (Cf-Ecp4) and S. ...

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The Solanum pimpinellifolium Cf-ECP1 and Cf-ECP4 genes for resistance to Cladosporium fulvum are located at the Milky Way locus on the short arm of chromosome 1

Cited by 21 publications

References 30 publications

Biochemical characterization of three distinct polygalacturonases from Neosartorya fischeri P1

Biochemical characterization of three distinct polygalacturonases from Neosartorya fischeri P1

Resistance-Gene-Mediated Defense Responses against Biotic Stresses in the Crop Model Plant Tomato

Genes encoding recognition of theCladosporium fulvumeffector protein Ecp5 are encoded at several loci in the tomato genome

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