The soluble extracellular domain of E‐cadherin interferes with EPEC adherence <i>via</i> interaction with the Tir:intimin complex

Login, Frédéric H.; Jensen, Helene Halkjær; Pedersen, Gitte A.; Amieva, Manuel R.; Nejsum, Lene N.

doi:10.1096/fj.201800651

Cited by 5 publications

(7 citation statements)

References 57 publications

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“…Previous research has shown that bacteria adhere to target cells as the first essential event in infection. 19,20 If attachment is established, the bacteria may utilize their potential to build a niche in order to be conducive to replication, colonization, and survival. 21,22 Then the cell-cycle alternation, apoptosis, could be observed in order to obtain persistent colonization during infection process.…”

Section: Discussionmentioning

confidence: 99%

Baicalin modulates apoptosis via RAGE, MAPK, and AP-1 in vascular endothelial cells during Haemophilus parasuis invasion

Zhao

Xiong

et al. 2019

Innate Immun

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Glässer’s disease, caused by Haemophilus parasuis, is a chronic disease related to an inflammatory immune response. Baicalin exerts important biological functions. In this study, we explored the protective efficacy of treatment with baicalin and the potential mechanism of activation of the MAPK signaling pathway in porcine aortic vascular endothelial cells (PAVECs) induced by H. parasuis. H. parasuis stimulated expression of receptor for advanced glycation end products, induced a significant increase in the level of protein kinase-α and protein kinase-δ phosphorylation, and significantly up-regulated ERK, c-Jun N-terminal kinase, and p38 phosphorylation in PAVECs. H. parasuis also up-regulated the levels of apoptotic genes ( Bax, C-myc, and Fasl) and the expression levels of c-Jun and c-Fos, and induced S-phase arrest in PAVECs. However, treatment with baicalin inhibited expression of RAGE, suppressed H. parasuis-induced protein kinase-α and protein kinase-δ phosphorylation, reduced ERK, c-Jun N-terminal kinase, and p38 phosphorylation, down-regulated apoptotic genes ( Bax, C-myc, and Fasl), attenuated phospho-c-Jun production from the extracellular to the nuclei, and reversed S-phase arrest in PAVECs. In conclusion, baicalin treatment inhibited the MAPK signaling pathway, thereby achieving its anti-inflammatory responses, which provides a new strategy to control H. parasuis infection.

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Section: Discussionmentioning

confidence: 99%

Baicalin modulates apoptosis via RAGE, MAPK, and AP-1 in vascular endothelial cells during Haemophilus parasuis invasion

Zhao

Xiong

et al. 2019

Innate Immun

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“…Following antibody labeling, the membrane pieces were rinsed three times in Krebs buffer and mounted on glass slides using Glycergel mounting medium (C0563, DAKO) containing antifade (1,4‐diazabicyclo‐[2,2,2]‐octane). Images of stained samples were acquired on an inverted Nikon Ti Eclipse fluorescent microscope equipped with a p E‐300 white broad‐spectrum LED illumination system, a Perfect Focus 3 system, CFI Plan Apochromat 100× (NA 1.45) oil objective and a Zyla 5.5 Megapixel camera (Andor Technology Ltd., Belfast, UK) as previously described 15–17 . Filtercubes for TxRed and Dapi were used for visualization.…”

Section: Methodsmentioning

confidence: 99%

“…Figure S1 shows the genuine set-up and a Zyla 5.5 Megapixel camera (Andor Technology Ltd., Belfast, UK) as previously described. [15][16][17] Filtercubes for TxRed and Dapi were used for visualization. Image processing was performed using the open source software IMAGEJ.…”

Section: Immunofluorescence Microscopymentioning

confidence: 99%

Streptococcus agalactiae do not penetrate human chorioamniotic membranes in vitro but alter their biomechanical properties

Mohamed

Hinge

Larsen

et al. 2021

Acta Obstet Gynecol Scand

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Introduction: Vaginal colonization with Streptococcus agalactiae (group B streptococci) is hypothesized to constitute a risk factor for preterm prelabor rupture of membranes.In vitro studies have shown that S. agalactiae strains isolated from infants with neonatal sepsis adhere to chorion cells of the human chorioamniotic membrane. However, it is still unknown whether S. agalactiae strains penetrate the chorioamniotic membranes and whether S. agalactiae colonization affects the biomechanical properties of the membranes and thus contributes to increased risk of preterm prelabor rupture.The aim of this in vitro study was to explore if different strains of S. agalactiae penetrate and affect the biomechanical properties of human chorioamniotic membranes.Material and methods: Three different strains of S. agalactiae were obtained, one from an early-onset neonatal infection, one from a case of preterm prelabor rupture of membranes and one from a healthy pregnant carrier. Chorioamniotic membranes from elective cesarean deliveries were either incubated with S. agalactiae or mounted in a two-chamber incubation cell generating a "maternal" and a "fetal" chamber and incubated with S. agalactiae in the maternal chamber. Subsequently the membranes were examined to evaluate S. agalactiae attachment, penetration and the effect on the biomechanical properties. Results: At 5 h after incubation, S. agalactiae adhered to the chorioamniotic membranes with increased number at 20 h. Streptococcus agalactiae did not penetrate the membranes even after 20 h of incubation. Streptococcus agalactiae increased the ultimate tensile stress needed to rupture the membranes and increased the work needed to rupture the membranes as well as the elastic modulus. Conclusions: Human chorioamniotic membranes constitute a physical barrier against S. agalactiae infections. Moreover, S. agalactiae infection leads to increased strength of the membranes. K E Y W O R D S chorioamniotic membranes, fetal membranes, group B streptococcus, perinatal infections, preterm prelabor rupture of membranes, Streptococcus agalactiae, tensile testing | 1815 MOHAMED Et Al.

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“…Immediately following its translocation into the host, Tir is targeted to the host cell membrane and becomes an integral membrane protein with two transmembrane domains and a surface-exposed loop. Intimin, the third product of the LEE5 operon, is an outer membrane protein that promotes adherence of EPEC to the host via interaction with the surface exposed loop of translocated Tir (11, 12). This attachment type was termed intimate attachment (11).…”

Section: Introductionmentioning

confidence: 99%

Activation of the type 3 secretion system of enteropathogenicE. colileads to remodeling of its membrane composition and function

Zacharia

Pal

Katsowich

et al. 2020

Preprint

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The type III secretion system (T3SS) is critical for the virulence of enteropathogenic E. coli (EPEC), an important human pathogen. T3SS is activated upon host attachment leading to effector injection, which is associated with repression of CsrA. CsrA repression results in gene expression reprogramming, which contributes to EPEC adaptation to a cell-adherent lifestyle. However, how this reprogramming influences EPEC physiology remains poorly understood. By combining genetic analyses, metabolomics and lipidomics, and data mining from reported datasets, we show that activation of the EPEC T3SS is associated with massive metabolic shifts, in particular, remodeling of lipid metabolism. The latter includes a shift from phospholipids towards lysophospholipids and cardiolipins and from ubiquinones and menaquinones production to the synthesis of undecaprenyl isoprenoids, concomitant to upregulation of O-antigen biosynthesis. We predict that the lipid remodeling upon attachment to host cells and T3SS activation contributes to bacterial fitness and promote host colonization.

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The soluble extracellular domain of E‐cadherin interferes with EPEC adherence via interaction with the Tir:intimin complex

Cited by 5 publications

References 57 publications

Baicalin modulates apoptosis via RAGE, MAPK, and AP-1 in vascular endothelial cells during Haemophilus parasuis invasion

Baicalin modulates apoptosis via RAGE, MAPK, and AP-1 in vascular endothelial cells during Haemophilus parasuis invasion

Streptococcus agalactiae do not penetrate human chorioamniotic membranes in vitro but alter their biomechanical properties

Activation of the type 3 secretion system of enteropathogenicE. colileads to remodeling of its membrane composition and function

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