The soluble hemagglutinins of adenoviruses belonging to Rosen's subgroup III

Hemagglutination with Adenovirus Serotypes Belonging to Rosen's Subgroups II and III

“…Hemagglutination and hemagglutination-inhibition tests were performed both in tubes (12 X 75 mm) as was previously described (5,15) and in disposable microtiter U-plates (Linbro Chem. Co. Inc., New Haven, Connecticut).…”

“…On the basis of this fact pentons were indicated by an antiserotype 3 dodecon serum. The Takatsy microtechnique for complement fixation (CF) was performed as previously described ( 5 ) .…”

“…All these polymers of structural components represent complexes, which are at least divalent with respect to their capacity to interact with receptors on red cells. On the other hand monovalent components, b., isolated pentons and fibers, were found to represent incomplete HA's (5,6,8). The fact that preparations of members of Rosen's subgroup I11 contain relatively more incomplete than complete HA's (5,6,8) was proposed as a possible explanation for the partial agglutination given by these serotypes.…”

“…In recent studies, the different complete HA's of adenovirus serotypes belonging to Rosen's subgroup I11 have been identified as virions (4,5), empty capsids (4), dodecons (representing the only soluble complete HA of serotype 4) (4, 6, 7 ) , and aggregates of two pentons or of two fibers (serotypes 1, 2, 5, and 6) (5,8,9). All these polymers of structural components represent complexes, which are at least divalent with respect to their capacity to interact with receptors on red cells.…”

“…Virus materials. Prototype strains of adenovirus types 1, 2, 3, 4, 5, 6, 9, 10, 11, and 15 were propagated in monolayer cultures of MAS-A cells, a human bone marrow cdl line, or a human embryonic lung cell line, Lu 106, and concentrated by use of the techniques previously described (5). The type specificity of the strains used was controlled in HI tests by use of reference sera obtained from the Department of Health, City of New York, N. Y. and kindly made available by Dr. Ake Espmark.…”