The SOUL family of heme-binding proteins: Structure and function 15 years later

Goodfellow, Brian J.; Freire, Filipe; Carvalho, Ana Luı́sa; Aveiro, Susana S.; Charbonnier, Peggy; Moulis, Jean‐Marc; Delgado, Leonildo; Ferreira, Glória C.; Rodrigues, João A.; Poussin-Courmontagne, Pierre; Birck, Catherine; McEwen, A.G.; Macedo, Anjos L.

doi:10.1016/j.ccr.2021.214189

Cited by 14 publications

(14 citation statements)

References 76 publications

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“…These include the Per-Arnt-Sim (PAS)-domain-containing hemoproteins (38, 39), such as the oxygen sensors Dos and FixL (40, 41). Other examples of α+β hemoproteins are the heme acquisition protein HasA in bacteria (42, 43) and the SOUL family of hemoproteins, which have proposed biological functions ranging from regulating cell fate to heme transport (44). There are exceptions to this theme, including the multifunctional α+β ferredoxin proteins (45, 46), but the majority of α+β hemoproteins are gas sensors or heme transporters.…”

Section: Discussionmentioning

confidence: 99%

Aim18p and Aim46p are CHI-domain-containing mitochondrial hemoproteins inSaccharomyces cerevisiae

Schmitz

Wolters

Murray

et al. 2022

Preprint

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Chalcone isomerases (CHIs) have well-established roles in the biosynthesis of plant flavonoid metabolites. Saccharomyces cerevisiae possesses two predicted CHI-like proteins, Aim18p (encoded by YHR198C) and Aim46p (YHR199C), but it lacks other enzymes of the flavonoid pathway, suggesting that Aim18p and Aim46p employ the CHI fold for distinct purposes. Here, we demonstrate that Aim18p and Aim46p reside on the mitochondrial inner membrane and adopt CHI folds, but they lack select active site residues and possess an extra fungal-specific loop. Consistent with these differences, Aim18p and Aim46p lack chalcone isomerase activity and also the fatty acid-binding capabilities of other CHI-like proteins, but instead bind heme. We further show that diverse fungal homologs also bind heme and that Aim18p and Aim46p possess structural homology to a bacterial hemoprotein. Collectively, our work reveals a distinct function and cellular localization for two CHI-like proteins, introduces a new variation of a hemoprotein fold, and suggests that ancestral CHI-like proteins were hemoproteins.

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Section: Discussionmentioning

confidence: 99%

Aim18p and Aim46p are CHI-domain-containing mitochondrial hemoproteins inSaccharomyces cerevisiae

Schmitz

Wolters

Murray

et al. 2022

Preprint

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show abstract

“…This was further supported by its correlation to a 67.6 ppm carbon via HSQC data and its correlation to aromatic carbons via the HMBC data. Based on this information, the 1 H and 13 C NMR data were assigned to the desired product as seen in Figure 3.9 thus suggesting the desired product was synthesized.…”

Section: Amine Alkylation Of 3-(6-bromohexyl)-15-dioxaspiro[55]undeca...mentioning

confidence: 99%

“…61,69,70 Ratio of 16 to hydrogen-bonded cyclohexane carboxyaldehyde is based on the ratio of 1 4.2. Summary of IR peaks (cm -1 ), 1 H chemical shifts (ppm) and 13 C chemical shifts Scheme 3.4. Synthesis of 3-hexyl-1,5-dioxaspiro [5.5]undecane-2,4-dione (18) using a twostep approach with coupling agent EDC•HCl.…”

Section: Acknowledgementsmentioning

confidence: 99%

“…[1][2][3][4][5] For example, proteomic studies can analyze reactive cysteine residues that are prone to oxidation by reactive oxygen species by reacting free cysteines with CD reagents with and without isotope incorporation. 8 The oxidation of cysteine on the protein can then be quantified by comparing the ratio of 12 C and 13 C labelled CDs as seen in Figure 1.1 A which can be later used to understand common diseases and conditions. 8 In targeted lipidomic studies, CD reagents incorporate isotopes to allow simultaneous analysis of multiple samples targeting free fatty acids as seen in Figure 1.1 B.…”

Section: Acknowledgementsmentioning

confidence: 99%

“…It is noted that the remainder of the spectrum demonstrated limited information as peaks within the alkane region did not show splitting patterns and were presented either as a singlet or a highly broad peak. Further characterization of the reaction was done by comparing the NMR to the starting materials as well as using COSY, 13 C NMR, HMBC and HSQC data.…”

Section: Amine Alkylation Of 3-(6-bromohexyl)-15-dioxaspiro[55]undeca...mentioning

confidence: 99%

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New Derivatization Strategies for Installation of Fixed, Permanent Charges onto Analytes of Biological and Environmental Concern

Aulenback¹

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Mass spectrometry (MS) is a highly sensitive analytical technique that can be used to identify thousands of analytes from a single biological matrix. Chemical derivatization (CD) reagents can enhance analyte detection and quantification by adding a specific functional group that enhances ionization efficiency and stability. The goal of this project is to design a new CD reagent by synthesizing a Meldrum's acid derivative containing a fixed permanent charge (MAD + ) to be used as a stable ketene precursor. Synthesis of a MAD + was explored via alkylation and Knoevenagel condensation reactions but proved unfruitful. However, Calkylation with 6-bromohexanoic acid via carbodiimide coupling, reduction and amine alkylation produced a MAD + in generous yields. The reaction of this MAD + with 3,5-di-tertbutylphenol demonstrated its ability to induce its fixed permanent charge onto analytes, allowing its detection in MS. Purification of the final MAD + remained an issue and should be investigated and optimized in future works.

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Comparative proteomics of the shell matrix proteins of Nautilus pompilius and the conchiferans provide insights into mollusk shell evolution at the molecular level

Hirota,

Tochino,

Seto

et al. 2023

Mar Biol

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The SOUL family of heme-binding proteins: Structure and function 15 years later

Cited by 14 publications

References 76 publications

Aim18p and Aim46p are CHI-domain-containing mitochondrial hemoproteins inSaccharomyces cerevisiae

Aim18p and Aim46p are CHI-domain-containing mitochondrial hemoproteins inSaccharomyces cerevisiae

New Derivatization Strategies for Installation of Fixed, Permanent Charges onto Analytes of Biological and Environmental Concern

Comparative proteomics of the shell matrix proteins of Nautilus pompilius and the conchiferans provide insights into mollusk shell evolution at the molecular level

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