The spa typing of methicillin-resistant Staphylococcus aureus isolates by High Resolution Melting (HRM) analysis

Fasihi, Yasser; Fooladi, Saba; Mohammadi, Mohammad Ali; Emaneini, Mohammad; Kalantar-Neyestanaki, Davood

doi:10.1099/jmm.0.000574

Cited by 14 publications

(24 citation statements)

References 11 publications

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“…This finding is in line with the findings of Hon-Kwan Chen et al study in China [8]. However, our results were in contrast with the results of Fasihi et al [11] and…”

Section: Resultssupporting

confidence: 89%

“…The MRSA isolates were obtained from patient's skin (19, 38%), Blood (10,20%), wound (8,16%), Sputum diagnostics. HRM method can easily and rapidly identify the different alleles of spa genes [11]. This method is used in a closed system and in the same tube as the amplification step; hence, it is faster, easier, and more suitable for high sample, which reduces the risk of contamination [18].…”

Section: Resultsmentioning

confidence: 99%

“…Different values of melting temperature were reported for one spa type. HRM method can be affected by testing conditions and type of real time machine as well as HRM software [9,11].…”

Section: Resultsmentioning

confidence: 99%

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Determining spa-type of methicillin-resistant Staphylococcus aureus (MRSA) via High-Resolution Melting (HRM) analysis, Shiraz, Iran

Hashemizadeh

Bazargani

Mohebi

et al. 2019

Preprint

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Objectives: Molecular typing methods are useful for rapid detection and control of a disease. Recently, the use of High-resolution melting (HRM) for spa typing of MRSA isolates were reported. This technique is rapid, inexpensive and simple for genotyping and mutation screening in DNA sequence. The aim of this study was to evaluate the ability of HRM-PCR to analysis spa genes amongst MRSA isolates.Results: A total of 50 MRSA isolates were collected from two teaching hospitals in Shiraz, Iran. The isolates were confirmed as MRSA by susceptibility to Cefoxitin and detection of mecA gene using PCR. we used High-resolution melting (HRM) analysis and PCR-sequencing method for spa typing of MRSA isolates. In total, 15 different spa types were discriminate by HRM and sequencing method. The melting temperature of the 15 spa types, using HRM genotyping were between 82.16° C and 85.66° C. The rate of GC% content was 39.4-46.3. According to the results, spa typing of 50 clinical isolates via PCR-sequencing and HRM methods were 100% similar. Consequently, HRM method can easily identify and rapidly differentiate alleles of spa genes. This method is faster, less laborious and more suitable for high sample at lower cost and risk of contamination.

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“…This finding is in line with the findings of Hon-Kwan Chen et al study in China [8]. However, our results were in contrast with the results of Fasihi et al [11] and…”

Section: Resultssupporting

confidence: 89%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Determining spa-type of methicillin-resistant Staphylococcus aureus (MRSA) via High-Resolution Melting (HRM) analysis, Shiraz, Iran

Hashemizadeh

Bazargani

Mohebi

et al. 2019

Preprint

Self Cite

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“…Nowadays, typing and analysis of MRSA strains have become a routine procedure in many countries and are being used as a tool to show the relationship between strains and clones. Although many MRSA genotypes and clonal clusters have been identified in different geographic regions, certain gene clones are predominant in some regions (26)(27)(28)(29)(30). For example, in the current study and among 26 MRSA isolates, we found 14 different genotypes.…”

Section: Discussioncontrasting

confidence: 44%

The spa Typing and Characterization of Nasal Carriage Methicillin-resistant Staphylococcus aureus Isolates From Healthy Children

Rezai¹,

Valadan²,

Ahangarkani³

et al. 2019

J. Pediatr. Rev.

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Background:The Methicillin-resistant Staphylococcus aureus (MRSA) strains cause wide spectrum infections in hospitals and are responsible for the majority of the communityacquired infections. The emergence of community-acquired MRSA in patients without risk factors represents a high risk for public health.Objectives: In this study, we aimed to molecular typing the nasal carriage MRSA strains harbored mecA gene isolated from healthy children living in the North of Iran and also to determine the pattern of vancomycin susceptibility in these isolates. Methods:The MRSA strains were identified using standard microbiological and molecular procedures. The antibiotic susceptibility test was performed according to the clinical and laboratory standards institute. The MRSA strains were characterized by spa typing. Results:In total, 14 different patterns of spa genes were obtained from MRSA isolates in this study, which included t790 (23.07%), t2962 (15.38%), t267 (15.38%), t159 (7.69%) and t701, t094, t1816, t11332, t937, t2135, t180, t160 [1 (3.84%) isolate per each type]. The rate of resistant isolates to cefoxitin, , cefalotin, clindamycin, cefazolin, co-amoxiclav, co-trimoxazole, and cephalexin antibiotics were 69.23%, 100%, 46.15%, 96.15%, 100%, 3.48%, and 100%, respectively. In total, six (23.07%) revealed vancomycin-intermediate resistant phenotype and five (19.23%) isolates revealed vancomycin-resistant S. aureus phenotype. Conclusions:This study showed the different variants of MRSA strains based on molecular typing, among healthy children. Continuous surveillance studies to monitor MRSA should be encouraged to gain a better understanding of the circulating MRSA strains and prevent community spread of this pathogen.

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“…This technique is rapid, inexpensive, simple and cost effective for genotyping and mutation screening in DNA sequence [7]. HRM with real-time polymerase chain reaction (PCR) can be used for spa typing hypervariable X region of the spa gene [7][8][9][10][11]. In this technique, DNA strands are distinguished based on the length and percentage of the GC content [11].…”

Section: Introductionmentioning

confidence: 99%

Determining spa-type of methicillin-resistant Staphylococcus aureus (MRSA) via high-resolution melting (HRM) analysis, Shiraz, Iran

et al. 2020

Self Cite

View full text Add to dashboard Cite

Objectives: Molecular typing methods are useful for rapid detection and control of a disease. Recently, the use of high-resolution melting (HRM) for spa typing of MRSA isolates were reported. This technique is rapid, inexpensive and simple for genotyping and mutation screening in DNA sequence. The aim of this study was to evaluate the ability of HRM-PCR to analysis spa genes amongst MRSA isolates. Results: A total of 50 MRSA isolates were collected from two teaching hospitals in Shiraz, Iran. The isolates were confirmed as MRSA by susceptibility to cefoxitin and detection of mecA gene using PCR. We used HRM analysis and PCR-sequencing method for spa typing of MRSA isolates. In total, 15 different spa types were discriminate by HRM and sequencing method. The melting temperature of the 15 spa types, using HRM genotyping were between 82.16 and 85.66 °C. The rate of GC % content was 39.4-46.3. According to the results, spa typing of 50 clinical isolates via PCR-sequencing and HRM methods were 100% similar. Consequently, HRM method can easily identify and rapidly differentiate alleles of spa genes. This method is faster, less laborious and more suitable for high sample at lower cost and risk of contamination.

show abstract

The spa typing of methicillin-resistant Staphylococcus aureus isolates by High Resolution Melting (HRM) analysis

Cited by 14 publications

References 11 publications

Determining spa-type of methicillin-resistant Staphylococcus aureus (MRSA) via High-Resolution Melting (HRM) analysis, Shiraz, Iran

Determining spa-type of methicillin-resistant Staphylococcus aureus (MRSA) via High-Resolution Melting (HRM) analysis, Shiraz, Iran

The spa Typing and Characterization of Nasal Carriage Methicillin-resistant Staphylococcus aureus Isolates From Healthy Children

Determining spa-type of methicillin-resistant Staphylococcus aureus (MRSA) via high-resolution melting (HRM) analysis, Shiraz, Iran

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