The sparing effect of phytase in plant-protein-based diets with decreasing supplementation of dietary NaH₂PO₄for juvenile yellow catfishPelteobagrus fulvidraco

Abstract

An 8‐week feeding trial was conducted to evaluate the sparing effect of dietary phytase on decreasing supplement of dietary sodium dihydrogen phosphate (NaH2PO4) in the diets of juvenile yellow catfish Pelteobagrus fulvidraco. Seven diets were formulated: one reference group that contained only 2% NaH2PO4; one control group that consisted of neither NaH2PO4 nor phytase; and five treatment groups supplemented with 1000 IU kg−1 phytase and 2%, 1.5%, 1%, 0.5%, 0% concentrations of NaH2PO4. Thus, dietary NaH2PO4 was substituted with phytase at ratios of 0%, 25%, 50%, 75% and 100% respectively. Triplicate tanks were each filled with 50 juvenile fish (3.5 ± 0.1 g), which were fed one of the seven experimental diets. Substituting phytase for NaH2PO4 didn't diminish the growth performance, protein, dry matter, lipid and ash deposit and protein retention in the whole body and reduced feed conversion ratio compared to the reference diet. Moreover, phytase substitution at level of 0–50% enhanced P, Mg and Ca deposit. However, weigh gain had a tendendy of decreasing with increasing substituted level. Phytase substituted level exceeding 75% decreased serum P concentration compared to the control group. Apparent digestibility of P peaked when 1000 IU kg−1 phytase substituted 50% NaH2PO4. Phytase also increased apparent digestibility of protein. The improvement of digestibility of protein and P resulted in the decrease in N load by 25% and P load by 31% to environment. Therefore, 50% dietary NaH2PO4 can be substituted by 1000 IU kg−1 feed phytase for juvenile yellow catfish.

EFFECT OF PHYTASE SUPPLEMENTED MORINGA BY-PRODUCTS BASED DIETS ON The PERFORMANCE OF OREOCHROMIS NILOTICUS FINGERLINGS

“…Closely similar to our results, maximum retention of protein, fat and gross energy were recorded at 900 FTU per kg addition of PHY in Catla catla fed Moringa by-products meal based diets (Shahzad et al 2016(Shahzad et al ,2018. Cheng et al (2015) recorded that plant based diets at 1000 FTU per kg addition of PHY show maximum protein and fat in Pelteobagrus fulvidraco (yellow catfish). However, Olusola and Nwanna (2014) strongly disagreed with these findings.…”

“…Shahzad et al (2016;2020) strongly agreed with the current results of the study and observed least contents of body ash, crude fiber and moisture in Catla catla were found at 900 FTU per kg addition of PHY in Moringa leaf meal based diets. Whereas, non-significant differences were observed by Cheng et al (2015) in fish body carcass irrespective of PHY addition. Nwanna et al (2008) in contrast found that, leucaena leaf meal and Brazil nut based diets irrespective of PHY addition showed non-significant differences in lipid contents of Colossoma macropomum, Amazon tambaqui fish.…”

Dietary phytase and protease improved growth and nutrient utilization in tilapia (Oreochromis niloticus × Oreochromis aureus) fed low phosphorus and fishmeal-free diets

“…Cheng et al. () found that 50% dietary NaH 2 PO 4 could be substituted by phytase (1,000 IU/kg feed) for juvenile yellow catfish, and the improvement of digestibility of protein and P by dietary phytase resulted in the decrease in N load by 25% and P load by 31% to environment. In the present study, the supplementation of phytase in LP (12 g/kg MCP) improved the growth, the digestibility of protein, P, and reached the similar level to the PC group with 18 g/kg MCP supplementation.…”

“…However, imbalanced amino acid profiles and presence of antinutritional factors such as phytate usually result in poor nutrient digestibility and thus severely limit their inclusion in feed. The overall quality of plant proteins can be improved using various enzymes, such as protease (Dalsgaard et al., ; Li, Chai, Liu, Chowdhury, & Leng, ; Shi, Li, Chowdhury, Chen, & Leng, ), phytase (Cheng, Chen, Lei, Feng, & Wang, ; Morales et al., ; Vandenberg, Scott, Sarker, Dallaire, & Noüe, ) and carbohydrase (Ai et al., ; Zhou et al., ).…”

“…Phytate can be hydrolysed by phytase to liberate inositol, inorganic P and other nutrients bound with phytic acid salt. The application of phytase has been widely reported in poultry (Mohebbifar, Torki, & Ghasemi, ; Olukosi, Cowieson, & Adeola, ), swine (Madrid, Martínez, López, & Hernández, ; Zeng et al., ), and various aquatic species, including rainbow trout ( Oncorhynchus mykiss ) (Morales et al., ; Vandenberg et al., ), rohu ( Labeo rohita ) (Hussain et al., ), grass carp ( Ctenopharyngodon idellus ) (Liu, Su, Zhang, Liang, & Luo, ), yellow catfish ( Pelteobagrus fulvidraco ) (Cheng et al., ) and Pacific white shrimp ( Litopenaeus vannamei ) (Qiu & Davis, ).…”