The Spatial Distribution of Monosomy 3 and Network Vasculogenic Mimicry Patterns in Uveal Melanoma

Abstract

Of the histologic prognostic factors of metastasis in UM studied, only the presence of a network vasculogenic mimicry pattern but not its location is associated with monosomy 3. This suggests that monosomy 3 may contribute to but is not sufficient for the development of the network pattern.

Concordant chromosome 3 results in paired choroidal melanoma biopsies and subsequent tumour resection specimens

“…Consistent with our current study, these data did not alter the overall chromosome 3 classification status, as compared with MLPA analysis of the whole tumour 22. Moreover, 14 CMs, in which two tumour regions were examined that differed phenotypically, demonstrated no intratumour heterogeneity for chromosome 3 classification, when examined with MSA 23. We previously reported one exceptional case where true heterogeneity existed within the tumour, with differing components of the CM displaying distinct morphological and genotypical features, which we believe had evolved over time 24.…”

In vivo monosomy 3 detection of posterior uveal melanoma: 3-year follow-up

“…The high percentage of monosomy 3 cells in each positive sample (mean, 89%± 9.4%) shows that, when present, monosomy 3 is fully exposed and easily detected by FNAB sampling. The presence of heterogeneous distribution of cytogenetic abnormalities, namely monosomy 3, has been recently evaluated in some case series, with conflicting results [25][26][27]. Maat et al used, on enucleated eyes, some laboratory techniques which may not represent the characteristics of in vivo tumors [26].…”

“…Maat et al used, on enucleated eyes, some laboratory techniques which may not represent the characteristics of in vivo tumors [26]. Meir et al, who used a larger pattern of laboratory investigation, were not able to detect tumor heterogeneity [27][28][29].…”

Effect of Heterogeneous Distribution of Monosomy 3 on Prognosis in Uveal Melanoma

“…8,19,20 Chromosomal heterogeneity can contribute to the metastatic potential of these tumors. We wanted to determine whether it was necessary for all cells to show monosomy 3 or if only a small subset of cells with monosomy 3 was enough for a bad prognosis.…”