Would physical location become less essential when work and communication are not limited to a fixed space? We explore the question with the micro-location choices of co-working spaces (CWSs) in London. The empirical results show that the CWS tends to choose districts with more 'teleworkable' jobs and a high reliance on tacit knowledge in local industries. CWS also tends to be located where start-up firms co-locate, echoing the different influences of knowledge spillover in firms' life cycles. The study highlights how CWS facilitates businesses in a digitised and more collaborative economy, carrying implications for business empowerment and local urban patterns.