The spatial relationship between the musculature and the 5-HT and FMRFamide immunoreactivities in cercaria, metacercaria and adult Opisthorchis felineus (Digenea)

Tolstenkov, Oleg; Теренина, Н. Б.; Сербина, Е. А.; Gustafsson, Margaretha K. S.

doi:10.2478/s11686-010-0024-4

Cited by 23 publications

(22 citation statements)

References 26 publications

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“…As expected, the 5-HT-IR fibers and neurons in all the investigated species form orthogonal patterns that are similar to those that have been reported from cercariae of other species (see Terenina et al 2006;Tolstenkov et al 2010Tolstenkov et al , 2011a. The number of paired 5-HT-IR neurons in the bodies of the investigated cercariae varied between five and eight, and between six and eight in the literature mentioned above.…”

Section: Discussionsupporting

confidence: 74%

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The neuromuscular system in freshwater furcocercaria from Belarus. II Diplostomidae, Strigeidae, and Cyathocotylidae

et al. 2011

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The neuromuscular system (NMS) in cercariae of Diplostomum pseudospathaceum, Cotylurus szidati, Australapatemon burti, Holostephanus volgensis, and Paracoenogonimus ovatus was studied with immunocytochemical methods and confocal scanning laser microscopy. The patterns of F-actin in the musculature, 5-HT immunoreactive (-IR), FMRF-amide-IR neuronal elements, and α-tubulin-IR in sensory receptors were investigated. The NMS in the five species studied were compared with each other and with three species of Schistosomatidae studied earlier (Bilharziella polonica, Trichobilharzia szidati, and Trichobilharzia franki). No major structural differences in the musculature, the 5-HT-IR or FMRF-IR neuronal elements were noticed between the cercariae. The minor variations observed in the musculature were related to the size and organization of the muscle fibers. The checked pattern formed by the transverse muscle fibers in the tail stems of D. pseudospathaceum, C. szidati, A. burti, H. volgensis, and P. ovatus was not observed in B. polonica, T. szidati, and T. franki. A trend in the differentiation of the longitudinal muscle fibers in the furca from evenly distributed fibers in H. volgensis and P. ovatus to many bundles in D. pseudospathaceum and two well-organized lateral bundles in C. szidati, A. burti, and Trichobilharzia spp. was observed. The transverse muscle fibers in the furca follow the same trend. The number of 5-HT-IR neurons in the cercarial bodies varied between 10 and 16. In cercariae of H. volgensis and P. ovatus, the central nervous system (CNS) was less centralized compared to the CNS in the other species studied, with only two 5-HT-IR marker neurons in each brain ganglion and the other neurons distributed evenly along the main cords. In the tails of H. volgensis and P. ovatus, many transverse 5-HT-IR comissures were found. In the tails of higher strigeidid cercariae, only a few crosslinks were observed. The number and distribution of sensory receptors on the bodies and tails of the cercarial species differed from each other. A trend in the differentiation of the sensory receptors in the tails was discerned. A process of grouping and decrease in number of ciliated receptors in the stem and in the furca from H. volgensis and P. ovatus to Schistosomatid cercariae took place.

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Section: Discussionsupporting

confidence: 74%

“…In the very long tail of P. ovatus, two very large 5-HT-IR neurons were observed. Neurons of this size category were observed in adult Opisthorchis felineus by Tolstenkov et al (2010). In the tails of H. volgensis and P. ovatus, many transverse 5-HT-IR comissures were found.…”

Section: Discussionmentioning

confidence: 95%

The neuromuscular system in freshwater furcocercaria from Belarus. II Diplostomidae, Strigeidae, and Cyathocotylidae

et al. 2011

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“…see Pan et 1994;Terenina et al 2006;Tolstenkov et al 2010). 5-HT-IR marker neurones were observed along the MCs in all species (Reuter et al 1998).…”

Section: Discussionmentioning

confidence: 95%

“…The number of 5-HT-IR neurones in the bodies of the investigated cercariae varies between 12 and 14, and between 12 and 17 in the literature mentioned above. In spite of size differences in the tails, the presence of two 5-HT-IR neurones seems to be a general rule (Terenina and Gustafsson 2003;Terenina et al 2006;Tolstenkov et al 2010). No major structural differences in the 5-HT-IR nervous systems were noticed between the cercaria studied.…”

Section: Discussionmentioning

confidence: 99%

“…The organisation of the neuro-muscular system (NMS) and the distribution of the neurotransmitters have been studied in several species of cercariae (Niewiadomska and Moczon 1982;Czubaj and Grabda-Kazubska 1993;Pan et al 1994;Terenina and Gustafsson 2003;Šebelová et al 2004;Terenina et al 2006;Tolstenkov et al 2010). In this study we compare the structure and composition of the NMS in five species of cercariae with different swimming patterns and strategies for finding and infecting their hosts.…”

Section: Introductionmentioning

confidence: 98%

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The neuro-muscular system in cercaria with different patterns of locomotion

et al. 2010

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The neuro-muscular system (NMS) of cercariae with different swimming patterns was studied with immunocytochemical methods and confocal scanning laser microscopy. Specimens of the continuously swimming Cercaria parvicaudata, Maritrema subdolum and Himasthla elongata were compared with specimens of the intermittently swimming Cryptocotyle lingua and the attached Podocotyle atomon. The patterns of F-actin in the musculature, 5-HT immunoreactive (-IR), FMRFamide-IR neuronal elements, α-tubulin-IR elements in the nervous and sensory systems and DAPI-stained nuclei were investigated. The general plan of the NMS was similar in all cercariae studied. No major structural differences in the patterns of muscle fibres were observed. However, in the tail of C. lingua, transverse muscle fibres connecting the bands of longitudinal muscles were found. No major structural differences in the 5-HT- or FMRFamide-IR nervous systems were observed. The number of 5-HT-IR neurones in the cercarial bodies varied between 12 and 14. The number and distribution of the α-tubulin-IR processes on the cercarial bodies and tails differed from each other. The relation between the number and structure of the α-tubulin-IR processes and the host finding strategy of the cercariae is discussed. A detailed schematic picture of the NMS in the tails of C. lingua and M. subdolum is presented.

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The neuroactive substances and associated muscle system in Rhipidocotyle campanula (Digenea, Bucephalidae) from the intestine of the pike Esox lucius

Kreshchenko

Теренина

Nefedova

et al. 2020

Journal of Morphology

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We report about the muscular system and the serotonergic and FMRFamidergic components of the nervous system of the Bucephalidae trematode, Rhipidocotyle campanula, an intestinal parasite of the pike. We use immunocytochemical methods and confocal scanning laser microscopy (CLSM). The musculature is identified by histochemical staining with fluorescently labeled phalloidin. The body wall musculature of R. campanula contains three layers of muscle fibresthe outer thin circular, intermediate longitudinal and inner diagonal muscle fibres running in two opposite directions. The digestive system of R. campanula possess of a well-developed musculature: radial, longitudinal and circular muscle elements are detected in the pharynx, circular and longitudinal muscle filaments seen in the oesophagus, and longitudinal and the circular muscle fibres were found in the intestinal wall. Specific staining indicating the presence of actin muscle filaments occurs in the cirrus sac localized in the posterior body region. The frontal region of anterior attachment organ, the rhynchus, in R. campanula is represented by radial muscle fibres. The posterior part of the rhynchus comprise of radial muscles forming the organ's wall, and several strong longitudinal muscle bundles. Serotonergic and FMRFamidergic structures are detected in the central and peripheral compartments of the nervous system of R. campanula, that is, in the paired brain ganglia, the brain commissure, the longitudinal nerve cords, and connective nerve commissures. The innervations of the rhynchus, pharynx, oesophagus and distal regions of the reproductive system by the serotonergic and FMRFamidergic nervous elements are revealed. We compare our findings obtained on R. campanula with related data for other trematodes.

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The spatial relationship between the musculature and the 5-HT and FMRFamide immunoreactivities in cercaria, metacercaria and adult Opisthorchis felineus (Digenea)

Cited by 23 publications

References 26 publications

The neuromuscular system in freshwater furcocercaria from Belarus. II Diplostomidae, Strigeidae, and Cyathocotylidae

The neuromuscular system in freshwater furcocercaria from Belarus. II Diplostomidae, Strigeidae, and Cyathocotylidae

The neuro-muscular system in cercaria with different patterns of locomotion

The neuroactive substances and associated muscle system in Rhipidocotyle campanula (Digenea, Bucephalidae) from the intestine of the pike Esox lucius

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