The specific capsular polysaccharide of <i>Streptococcus pneumoniae</i> type 9V

Perry, Malcolm B.; Daoust, Virginia; Carlo, Dennis J.

doi:10.1139/o81-073

“…To the completed oligosaccharide unit, additional O-acetyl groups may be variably attached to the ManNAc, glucuronic acid and glucose residues (Fig. 2) (Perry et al, 1981 ;Rutherford et al, 1991) by the putative O-acetyltransferases Cps9vM and Cps9vO. After transport across the membrane facilitated by Cps9vK, the repeating units are likely polymerized via the Cps9vI polymerase.…”

Section: Discussionmentioning

confidence: 99%

“…Structure of the repeating unit of the CPS of S. pneumoniae serotype 9V (Perry et al, 1981 ;Rutherford et al, 1991). Glc, glucose ; GlcA, glucuronic acid ; Gal, galactose ; ManNAc, 2-acetamido-2-deoxymannose ; OAc, O-acetyl. that convert UDP-glucose to UDP-glucuronic acid (Campbell et al, 2000b).…”

Section: Figmentioning

confidence: 99%

Organization and characterization of the capsule biosynthesis locus of Streptococcus pneumoniae serotype 9V The GenBank accession number for the sequence reported in this paper is AF402095.

Selm

¹

,

Kolkman²,

Zeijst

³

et al. 2002

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The capsular polysaccharide (CPS) synthesis locus of Streptococcus pneumoniae serotype 9V was amplified by long-range PCR and sequenced. The locus was 17 368 bp in size and contained 15 ORFs. The genetic organization of the cluster shared many features with other S. pneumoniae capsule loci, including the presence of four putative regulatory genes at the 5' end. Comparative sequence analyses allowed putative functions to be assigned to each of the gene products. The ORFs appeared to encode, besides the four regulatory genes, five glycosyltransferases, two O-acetyltransferases, an Nacetylglucosamine 2-epimerase, a glucose 6-dehydrogenase, an oligosaccharide transporter protein and a polysaccharide repeating unit polymerase. These functions covered the steps proposed in the CPS biosynthesis of serotype 9V. TLC of carbohydrate intermediates formed after incubation of bacterial membrane preparations with14 C-labelled precursors demonstrated that the fifth ORF (cps9vE) encoded a UDP-glucosyl-1-phosphate transferase. This function was confirmed with the help of a cps9vE mutant that carried a deletion of a guanine residue located adjacent to a stretch of adenines. The identification and characterization of the serotype 9V locus is a major step in unravelling the 9V capsule biosynthesis pathway and broadens the insight into the genetic diversity of the S. pneumoniae capsule loci.

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“… Structures of the repeating units of S. pneumoniae group 9 PS as reported by Jones et al [10](type 9N), Richards et al [12](type 9L), Richards and Perry [13](type 9A) and Perry et al [11]and Rutherford et al [7](type 9V). …”

Section: Resultsmentioning

confidence: 91%

“…1. Each group 9 PS is a high molecular mass polymer with a linear pentasaccharide repeating unit [7, 10–13]. Residues are labelled A–E for convenient specification of atom numbering.…”

Section: Resultsmentioning

confidence: 99%

Monoclonal antibodies with specificities forStreptococcus pneumoniaegroup 9 capsular polysaccharides

Kolberg¹,

Jones

²

1998

FEMS Immunology &Amp; Medical Microbiology

7

0

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Streptococcus pneumoniae group 9 includes four capsular polysaccharide types: 9A, 9L, 9N and 9V. We have generated four mouse monoclonal antibodies against group 9 polysaccharide using heat-treated S. pneumoniae strains of different capsular polysaccharides types as immunogens. The specificities of the monoclonal antibodies were determined by ELISA using capsular polysaccharide directly coated to the wells as antigens and by dot blotting with heat-treated bacteria. Two groups of monoclonal antibodies were found. The first group included two monoclonal antibodies which were found to be capsular type specific. The second group was monoclonal antibodies that bound to epitopes shared by two or three pneumococcal group 9 types. The monoclonal antibody 204,A-4 (IgM) was found to be specific for S. pneumoniae type 9N. The binding of the type 9V specific monoclonal antibody 206,F-5 (IgG1) was found to be dependent upon O-acetyl groups. Monoclonal antibody 205,F-3 (IgM) reacted also with type 9V, but was found to cross-react with types 9A and 9L. The binding of this monoclonal antibody to polysaccharide 9V was not dependent upon O-acetyl moieties. The fourth monoclonal antibody (214,G-5, isotype IgM) did not show any correlation between reactivity with isolated polysaccharides and dot blotting with relevant bacteria. The monoclonal antibody reacted with polysaccharides 9A and 9L in ELISA, but not with the homologous bacteria.

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“…The immunostaining was performed with 3-amino-9-ethylcarbazole and H P O P in sodium acetate bu¡er (pH 5.0). [12] (type 9L), Richards and Perry [13] (type 9A) and Perry et al [11] and Rutherford et al [7] (type 9V).…”

Section: Dot Blot Assaymentioning

confidence: 94%

Monoclonal antibodies with specificities for Streptococcus pneumoniae group 9 capsular polysaccharides

Kolberg¹

1998

FEMS Immunology and Medical Microbiology

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Streptococcus pneumoniae group 9 includes four capsular polysaccharide types: 9A, 9L, 9N and 9V. We have generated four mouse monoclonal antibodies against group 9 polysaccharide using heat-treated S. pneumoniae strains of different capsular polysaccharides types as immunogens. The specificities of the monoclonal antibodies were determined by ELISA using capsular polysaccharide directly coated to the wells as antigens and by dot blotting with heat-treated bacteria. Two groups of monoclonal antibodies were found. The first group included two monoclonal antibodies which were found to be capsular type specific. The second group was monoclonal antibodies that bound to epitopes shared by two or three pneumococcal group 9 types. The monoclonal antibody 204,A-4 (IgM) was found to be specific for S. pneumoniae type 9N. The binding of the type 9V specific monoclonal antibody 206,F-5 (IgG1) was found to be dependent upon O-acetyl groups. Monoclonal antibody 205,F-3 (IgM) reacted also with type 9V, but was found to cross-react with types 9A and 9L. The binding of this monoclonal antibody to polysaccharide 9V was not dependent upon O-acetyl moieties. The fourth monoclonal antibody (214,G-5, isotype IgM) did not show any correlation between reactivity with isolated polysaccharides and dot blotting with relevant bacteria. The monoclonal antibody reacted with polysaccharides 9A and 9L in ELISA, but not with the homologous bacteria.

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The specific capsular polysaccharide of Streptococcus pneumoniae type 9V

Cited by 44 publications

References 0 publications

Organization and characterization of the capsule biosynthesis locus of Streptococcus pneumoniae serotype 9V The GenBank accession number for the sequence reported in this paper is AF402095.

Organization and characterization of the capsule biosynthesis locus of Streptococcus pneumoniae serotype 9V The GenBank accession number for the sequence reported in this paper is AF402095.

Monoclonal antibodies with specificities forStreptococcus pneumoniaegroup 9 capsular polysaccharides

Monoclonal antibodies with specificities for Streptococcus pneumoniae group 9 capsular polysaccharides

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