The specific expression of Hypoxia Inducible Factor‐1α in human gastric mucosa induced by nonsteroidal anti‐inflammatory drugs

Ito, Masanori; Tanaka, S; Kim, S.; Kuwai, Toshio; Matsutani, Norimasa; Kamada, Takenobu; Kitadai, Yasuhiko; Sumii, Masaharu; Yoshihara, Masaharu; Haruma, Ken; Chayama, Kazuaki

doi:10.1046/j.1365-2036.18.s1.10.x

Cited by 20 publications

(14 citation statements)

References 31 publications

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“…It occurs via increased messenger ribonucleic acid (mRNA) expression, nuclear localization, and augmented activity of transcriptional activation domains. Nuclear accumulation of this protein can be detected immunohistochemically [9][10][11]. Vascular endothelial growth factor (VEGF) is known to be a potent angiogenic mitogen that plays an important role in tumor angiogenesis [12] and a multifunctional cytokine that stimulates angiogenesis and increases microvascular permeability through binding to specific receptors expressed on vascular endothelial cells.…”

Section: Introductionmentioning

confidence: 98%

Expression of hypoxia-inducible factor-1 alpha (HIF-1α) in patients with the gallbladder carcinoma

et al. 2010

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Section: Introductionmentioning

confidence: 98%

Expression of hypoxia-inducible factor-1 alpha (HIF-1α) in patients with the gallbladder carcinoma

et al. 2010

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“…Mixed antigens, 100 µg of each, were injected 8 times subcutaneously every 2 weeks into 2 rabbits (Immuno-Biological Laboratories Co. Ltd., Gunma, Japan). Whole serum was collected 15 weeks after the first immunization and was subjected to an enzyme-linked immunosorbent assay using antigen peptide, as previously described [19]. The minimum dilution of the antiserum with a positive reaction was 1/51,200, whereas that of the preimmunized serum was 1/100.…”

Section: Methodsmentioning

confidence: 99%

Role of the Gastrin-Gastrin Receptor System in the Expansive Growth of Human Gastric Neoplasms

et al. 2008

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Background and Aim: Gastrin is one of the most important gut hormones. However, the role of the gastrin-gastrin receptor (GR) system in the growth of gastric tumors is still unclear. Methods: We examined serum gastrin levels in 957 patients with early gastric carcinoma. Next, we raised antibody against the GR and examined GR expression in 5 gastric carcinoma cell lines and 48 human gastric tumor tissues. In 28 cases, Helicobacter pylori eradication therapy was performed and morphological tumor changes were examined. Results: Serum gastrin levels were significantly higher in patients with elevated tumors than in patients with depressed tumors (p = 0.02). All gastric carcinoma cell lines expressed GR. Thirty-one of 48 (65%) gastric tumors expressed GR, and its expression was prominent in elevated-type tumor with an intestinal histologic feature. Of 28 patients who underwent eradication therapy, 9 showed gastric tumors that became flat or depressed. In these 9 cases, GR expression was detected in all tumors, and the decrease in gastrin levels was more prominent than in those without morphological change (p = 0.01). Conclusion: The gastrin-GR system plays an important role in the elevated morphology of gastric tumors.

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“…Western blot analysis revealed the HIF-1· expression to occur in a radical oxygen species-dependent manner (25) in gastric cancer cells, and to be induced by Cysteamine (26) or by non-steroidal antiinflammatory drugs (27) in the gastric mucosa. Studies are still necessary to elucidate precisely how the expression of HIF-1· is regulated and the role of this subunit in human gastric carcinoma.…”

Section: Introductionmentioning

confidence: 99%

CoCl2-induced HIF-1α expression correlates with proliferation and apoptosis in MKN-1 cells: A possible role for the PI3K/Akt pathway

Ardyanto

Osaki²,

Tokuyasu³

et al. 2006

Int J Oncol

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Abstract. The exact mechanism behind the effect of hypoxiainducible factor-1· (HIF-1·) on the proliferation and/or apoptosis of carcinoma cells is still a matter of debate. We treated a human gastric carcinoma cell line, MKN-1 (mutant P53), with 500 μM CoCl 2 . A dual-phase pattern of HIF-1· expression with an increase until 4 h followed by a decrease until 36 h was observed. Immunocytochemistry showed that nuclear translocation was maximal at 4 h of treatment, while trypan blue staining showed a dual-phase pattern. Instead of G 1 /S arrest, FACS showed an increase in the pre-G 1 fraction and G 2 /M arrest that correlated with Cyclin-B1, SKP-2 and P27 expression. Starting at 6 h, the apoptotic index increased in a time-dependent manner, in correlation with the expression of HIF-1·, Bcl-2, Bcl-xL, Bax and cleaved-Caspase-9. Phosphorylation of Akt was inhibited by CoCl 2 treatment and LY294002 treatment inhibited HIF-1· expression in a dosedependent manner. These results suggested that the alteration of CoCl 2 -induced HIF-1· expression correlated with proliferation and apoptosis in MKN-1 cells. A possible role for the PI3K/Akt pathway was indicated in this model

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The specific expression of Hypoxia Inducible Factor‐1α in human gastric mucosa induced by nonsteroidal anti‐inflammatory drugs

Cited by 20 publications

References 31 publications

Expression of hypoxia-inducible factor-1 alpha (HIF-1α) in patients with the gallbladder carcinoma

Expression of hypoxia-inducible factor-1 alpha (HIF-1α) in patients with the gallbladder carcinoma

Role of the Gastrin-Gastrin Receptor System in the Expansive Growth of Human Gastric Neoplasms

CoCl2-induced HIF-1α expression correlates with proliferation and apoptosis in MKN-1 cells: A possible role for the PI3K/Akt pathway

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