The specific localizations of phosphorylated Lhcb1 and Lhcb2 isoforms reveal the role of Lhcb2 in the formation of the PSI-LHCII supercomplex in Arabidopsis during state transitions

Crepin, Aurélie; Caffarri, Stefano

doi:10.1016/j.bbabio.2015.09.005

Cited by 59 publications

(64 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, LHCII-PSI-LHCI supercomplexes have been successfully isolated and purified using various detergents (Galka et al, 2012;Drop et al, 2014;Crepin and Caffarri, 2015) or a styrene-maleic acid copolymer (Bell et al, 2015). These findings yielded further insights into the reorganization of supercomplexes associated with state transitions, and it was suggested that phosphorylation of LHCB2 rather than LHCB1 is the essential trigger for the formation of state transition supercomplexes (Leoni et al, 2013;Pietrzykowska et al, 2014;Crepin and Caffarri, 2015;Longoni et al, 2015). Furthermore, characterization of mutants deficient in individual PSI core subunits indicates that PsaH, L, and I are required for docking of LHCII at PSI (Lunde et al, 2000;Zhang and Scheller, 2004;Kouril et al, 2005;Plöchinger et al, 2016).…”

mentioning

confidence: 99%

Comparative Analysis of Light-Harvesting Antennae and State Transition in chlorina and cpSRP Mutants

Wang

Grimm

2016

Plant Physiol.

View full text Add to dashboard Cite

State transitions in photosynthesis provide for the dynamic allocation of a mobile fraction of light-harvesting complex II (LHCII) to photosystem II (PSII) in state I and to photosystem I (PSI) in state II. In the state I-to-state II transition, LHCII is phosphorylated by STN7 and associates with PSI to favor absorption cross-section of PSI. Here, we used Arabidopsis (Arabidopsis thaliana) mutants with defects in chlorophyll (Chl) b biosynthesis or in the chloroplast signal recognition particle (cpSRP) machinery to study the flexible formation of PS-LHC supercomplexes. Intriguingly, we found that impaired Chl b biosynthesis in chlorina1-2 (ch1-2) led to preferentially stabilized LHCI rather than LHCII, while the contents of both LHCI and LHCII were equally depressed in the cpSRP43-deficient mutant (chaos). In view of recent findings on the modified state transitions in LHCI-deficient mutants (Benson et al., 2015), the ch1-2 and chaos mutants were used to assess the influence of varying LHCI/LHCII antenna size on state transitions. Under state II conditions, LHCII-PSI supercomplexes were not formed in both ch1-2 and chaos plants. LHCII phosphorylation was drastically reduced in ch1-2, and the inactivation of STN7 correlates with the lack of state transitions. In contrast, phosphorylated LHCII in chaos was observed to be exclusively associated with PSII complexes, indicating a lack of mobile LHCII in chaos. Thus, the comparative analysis of ch1-2 and chaos mutants provides new evidence for the flexible organization of LHCs and enhances our understanding of the reversible allocation of LHCII to the two photosystems.

show abstract

mentioning

confidence: 99%

Comparative Analysis of Light-Harvesting Antennae and State Transition in chlorina and cpSRP Mutants

Wang

Grimm

2016

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…A proportion of LHC II serves as the antenna of photosystem I in Arabidopsis chloroplasts adapted to increased light intensity (Wientjes et al ). In addition, individual LHC II trimers, termed S and M, remain connected to photosystem II upon phosphorylation, indicating that specific trimers migrate to photosystem I (Wientjes et al , Crepin and Caffarri ). Trimers may be composed of different individual polypeptide chains, giving homotrimers and heterotrimers with specific mobilities and kinetics of phosphorylation (Leoni et al , Pietrzykowska et al , Crepin and Caffarri ).…”

Section: The Photosystem II Supercomplexmentioning

confidence: 99%

“…In addition, individual LHC II trimers, termed S and M, remain connected to photosystem II upon phosphorylation, indicating that specific trimers migrate to photosystem I (Wientjes et al , Crepin and Caffarri ). Trimers may be composed of different individual polypeptide chains, giving homotrimers and heterotrimers with specific mobilities and kinetics of phosphorylation (Leoni et al , Pietrzykowska et al , Crepin and Caffarri ). In Arabidopsis , trimers termed M and S remain bound the photosystem II supercomplex even when phosphorylated on chains Lhcb‐1 and Lhcb‐2 (Crepin and Caffarri ).…”

Section: The Photosystem II Supercomplexmentioning

confidence: 99%

“…Trimers may be composed of different individual polypeptide chains, giving homotrimers and heterotrimers with specific mobilities and kinetics of phosphorylation (Leoni et al , Pietrzykowska et al , Crepin and Caffarri ). In Arabidopsis , trimers termed M and S remain bound the photosystem II supercomplex even when phosphorylated on chains Lhcb‐1 and Lhcb‐2 (Crepin and Caffarri ).…”

Section: The Photosystem II Supercomplexmentioning

confidence: 99%

“…In Arabidopsis , the LHC II L‐trimer (‘L’ for ‘loosely‐bound’ to photosystem II) contains Lhcb‐1 and Lhcb‐2 chains and forms part of the antenna of photosystem I when Lhcb‐2 is phosphorylated (Crepin and Caffarri ). Crepin and Caffarri () conclude that phosphorylation of only one polypeptide chain of the L‐trimer is sufficient for formation of the LHC II‐PS I complex; that this chain is Lhcb2; and that its binding site with photosystem I involves a direct interaction of the phosphothreonine with PsaH.…”

Section: Resolving Molecular Recognition Between Lhc II and Photosystmentioning

confidence: 99%

See 2 more Smart Citations

Why we need to know the structure of phosphorylated chloroplast light‐harvesting complex II

Allen

2017

Physiologia Plantarum

View full text Add to dashboard Cite

In oxygenic photosynthesis there are two 'light states' -adaptations of the photosynthetic apparatus to spectral composition that otherwise favours either photosystem I or photosystem II. In chloroplasts of green plants the transition to light state 2 depends on phosphorylation of apoproteins of a membrane-intrinsic antenna, the chlorophyll-a/b-binding, light-harvesting complex II (LHC II), and on the resulting redistribution of absorbed excitation energy from photosystem II to photosystem I. The transition to light state 1 reverses these events and requires a phospho-LHC II phosphatase. Current structures of LHC II reveal little about possible steric effects of phosphorylation. The surface-exposed N-terminal domain of an LHC II polypeptide contains its phosphorylation site and is disordered in its unphosphorylated form. A molecular recognition hypothesis proposes that state transitions are a consequence of movement of LHC II between binding sites on photosystems I and II. In state 1, LHC II forms part of the antenna of photosystem II. In state 2, a unique but as yet unidentified 3-D structure of phospho-LHC II may attach it instead to photosystem I. One possibility is that the LHC II N-terminus becomes ordered upon phosphorylation, adopting a local alpha-helical secondary structure that initiates changes in LHC II tertiary and quaternary structure that sever contact with photosystem II while securing contact with photosystem I. In order to understand redistribution of absorbed excitation energy in photosynthesis we need to know the structure of LHC II in its phosphorylated form, and in its complex with photosystem I.

show abstract

Photoprotection and growth under different lights of Arabidopsis single and double mutants for energy dissipation (npq4) and state transitions (pph1)

2019

Self Cite

View full text Add to dashboard Cite

Non Photochemical Quenching (NPQ) is a short-term regulation important to maintain efficient photosynthesis and to avoid photooxydative damages by dissipation of excess energy. Full activation of NPQ in plants requires the protonation of the PsbS protein, which is the sensor of the low lumenal pH triggering the thermal dissipation. State transitions are a second important photosynthetic regulation to respond to changes in light quality and unbalanced excitation of photosystems. State transitions allow energy redistribution between PSI and PSII through the reversible exchange of LHCII antenna complexes between photosystems thank to the opposite action of the STN7 kinase and PPH1 phosphatase: phosphorylation of LHCII promotes its mobilization from PSII to PSI, while dephosphorylation has the opposite effect. In this work, we produced the pph1/npq4 double mutant and characterized some photosynthetic, growth and reproduction properties in comparison with wild type and single mutant plants in high and low light conditions. Results indicate that in high light, the pph1 mutant maintains good photoprotection ability, while npq4 plants show more susceptibility to photodamages. The pph1/npq4 double mutant showed a resistance to high light stress similar to that of the single npq4 mutant. In low light condition, the single mutants showed a significant increase of growth and flowering as compared to wild type plants and this effect was further enhanced in the pph1/npq4 double mutant. Results suggest that photosynthetic optimisation to improve crop growth and productivity might be possible, at least under controlled low light conditions, by modifying NPQ and state transitions regulations.

show abstract

The specific localizations of phosphorylated Lhcb1 and Lhcb2 isoforms reveal the role of Lhcb2 in the formation of the PSI-LHCII supercomplex in Arabidopsis during state transitions

Cited by 59 publications

References 57 publications

Comparative Analysis of Light-Harvesting Antennae and State Transition in chlorina and cpSRP Mutants

Comparative Analysis of Light-Harvesting Antennae and State Transition in chlorina and cpSRP Mutants

Why we need to know the structure of phosphorylated chloroplast light‐harvesting complex II

Photoprotection and growth under different lights of Arabidopsis single and double mutants for energy dissipation (npq4) and state transitions (pph1)

Contact Info

Product

Resources

About