The spectrographic determination of linoleic, linolenic and elaeostearic acids

Hilditch, T. P.; Morton, R. A.; Riley, J.P.

doi:10.1039/an9457000068

Cited by 90 publications

(21 citation statements)

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“…Mixed fatty acids of P. sornniferurn seed oil and also its fractions (A-D) were examined spectrophotometrically after alkali isomerisation, for their contents of polyethenoid acids. 15,16 Results are given in Table 1. -Lipolysis of the P. sornniferurn seed oil was carried out as suggested by Colenian17 at pH 8.5 and 37.5"C using a purified pancreatic lipase preparation with the addition of Ca2+ ions and bile salts.…”

Section: Experimental and Resultsmentioning

confidence: 99%

Triglyceride composition of Papaver somniferum seed oil

Sengupta

Mazumder

1976

J Sci Food Agric

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Section: Experimental and Resultsmentioning

confidence: 99%

Triglyceride composition of Papaver somniferum seed oil

Sengupta

Mazumder

1976

J Sci Food Agric

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“…Beadle and Kraybill (2) extended it to include determination of araehidonic acid. have been described by Hilditch et al (9), Baldwin and Longenecker (10), Holman and Burr (11), Berk el al. O'Connor et al improved the purity of standards by excluding oxygen in their preparation (7) and greatly improved the transparency of the KOH-ethylene glycol isomerization medium by protection with an atmosphere of nilrogen (8).…”

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confidence: 79%

Standardization of spectrophotometric methods for determination of polyunsaturated fatty acids using pure natural acids

Brice

Swain

Herb

et al. 1952

J Americ Oil Chem Soc

114

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Summary Spectrophotometric methods of analysis for the polyunsaturated constituents of oils and fats have been carefully restandardized for several conditions of alkali‐isomerization, using purified methyl esters of linoleic, linolenic, and arachidonic acids prepared by physical rather than by chemical means. A number of vegetable oil and animal fat samples were subjected to spectrophotometric analysis, and the results based on natural and on bromination‐debromination fatty acid standards were compared. The natural fatty acid standards lead to significantly higher accuracy and their use in the spectrophotometric analysis of natural fatty materials is strongly recommended. Results obtained under different conditions of isomerization were in satisfactory agreement. An isomerization time of 45 minutes is recommended rather than 25 or 30 minutes. The glycerol‐air technique is preferred for general use because of its simplicity and high precision. The ethylene‐glycol‐nitrogen technique is a close second choice because of the greater transparency of reagent blanks.

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“…The absence of higher unsaturated fatty acids (linoleic, linolenic and arachidonic acids) was indicated, since no distinct increase in ultra-violet absorption was detected in the regions 2,350 A, 2,700 A and 3,000 A (Wiese and Hansen, 1953), after treatment of the G acid with alkali by the method of Hilditch, Morton, and Riley (1945).…”

Section: Resultsmentioning

confidence: 99%

OBSERVATIONS ON A SUBSTANCE IN HUMAN PLASMA WHICH GIVES A SLOW CONTRACTION OF GUINEA‐PIG GUT IN VITRO

1956

British Journal of Pharmacology and Chemotherapy

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The spectrographic determination of linoleic, linolenic and elaeostearic acids

Cited by 90 publications

References 0 publications

Triglyceride composition of Papaver somniferum seed oil

Triglyceride composition of Papaver somniferum seed oil

Standardization of spectrophotometric methods for determination of polyunsaturated fatty acids using pure natural acids

OBSERVATIONS ON A SUBSTANCE IN HUMAN PLASMA WHICH GIVES A SLOW CONTRACTION OF GUINEA‐PIG GUT IN VITRO

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