The Sperm-Induced Ca2+ Wave Following Fertilization of the Xenopus Egg Requires the Production of Ins(1,4,5)P3

Nuccitelli, Richard; Yim, Darlene L.; Smart, Tanya

doi:10.1006/dbio.1993.1179

Cited by 174 publications

(121 citation statements)

References 0 publications

Supporting

Mentioning

113

Contrasting

Unclassified

Order By: Relevance

“…In Xenopus, the situation is less complicated with only a single Ca 2þ release pathway detected functionally and biochemically, the type 1 IP 3 R (Parys et al, 1992;Parys and Bezprozvanny, 1995). As is the case in mammals, IP 3 -dependent Ca 2þ release is essential for egg activation in Xenopus (Larabell and Nuccitelli, 1992;Nuccitelli et al, 1993;Runft et al, 2002).…”

Section: Ca 2r Signalingmentioning

confidence: 99%

“…The Ca 2þ wave is followed by a sustained Ca 2þ plateau that lasts for several minutes (Busa and Nuccitelli, 1985;El Jouni et al, 2005). The Ca 2þ wave is due to Ca 2þ release from intracellular stores through the IP 3 receptor (IP 3 R), since it is inhibited by blocking IP 3 -dependent Ca 2þ release or PIP2 hydrolysis (Larabell and Nuccitelli, 1992;Nuccitelli et al, 1993;Runft et al, 1999). Moreover, a wave of IP 3 has been proposed to precede the Ca 2þ release wave in eggs at fertilization (Wagner et al, 2004).…”

Section: Spatial and Temporal Dynamics Of Ca 2r Signals During Maturamentioning

confidence: 99%

“…Furthermore, the Ca 2þ wave propagates at a more rapid rate in oocytes ($20 mm/sec) compared to eggs ($9 mm/sec; Fontanilla and Nuccitelli, 1998;Machaca, 2004), and the mode of wave propagation is modulated during maturation. In oocytes wave propagation is proposed to be saltatory (Lechleiter and Clapham, 1992;Callamaras et al, 1998;Machaca, 2004), whereas it appears to be continuous in eggs (Nuccitelli et al, 1993;Fontanilla and Nuccitelli, 1998;Machaca, 2004). The building blocks of Ca 2þ waves are quantal elementary Ca 2þ release events due to the opening of a few IP 3 receptors that in Xenopus are referred to as Ca 2þ puffs (Parker and Yao, 1995).…”

Section: Spatial and Temporal Dynamics Of Ca 2r Signals During Maturamentioning

confidence: 99%

See 2 more Smart Citations

Ca²⁺ signaling differentiation during oocyte maturation

Machaca

2007

Journal Cellular Physiology

View full text Add to dashboard Cite

Oocyte maturation is an essential cellular differentiation pathway that prepares the egg for activation at fertilization leading to the initiation of embryogenesis. An integral attribute of oocyte maturation is the remodeling of Ca 2þ signaling pathways endowing the egg with the capacity to produce a specialized Ca 2þ transient at fertilization that is necessary and sufficient for egg activation. Consequently, mechanistic elucidation of Ca 2þ signaling differentiation during oocyte maturation is fundamental to our understanding of egg activation, and offers a glimpse into Ca 2þ signaling regulation during the cell cycle.

show abstract

Section: Ca 2r Signalingmentioning

confidence: 99%

Section: Spatial and Temporal Dynamics Of Ca 2r Signals During Maturamentioning

confidence: 99%

Section: Spatial and Temporal Dynamics Of Ca 2r Signals During Maturamentioning

confidence: 99%

See 1 more Smart Citation

Ca²⁺ signaling differentiation during oocyte maturation

Machaca

2007

Journal Cellular Physiology

View full text Add to dashboard Cite

show abstract

“…Finally, it may be that, although the NO pathway itself is widely distributed (52)(53)(54), its involvement at fertilization is confined to echinoderms or perhaps echinoids. Neither frog (55), ascidian (56), nor mammalian oocytes (57, 58) rely on the RyR receptor at fertilization, and neither mouse nor ascidian oocytes showed any evidence of increases in NO at fertilization (27). One obvi- The mean latent period (ϮS.E.…”

Section: Measurements Of Cadpr Levels At Fertilization Suggest Cadpr mentioning

confidence: 99%

The NO Pathway Acts Late during the Fertilization Response in Sea Urchin Eggs

Leckie

Empson

Becchetti

et al. 2003

Journal of Biological Chemistry

View full text Add to dashboard Cite

Both the inositol 1,4,5-trisphosphate (InsP 3 ) and ryanodine receptor pathways contribute to the Ca 2؉ transient at fertilization in sea urchin eggs. To date, the precise contribution of each pathway has been difficult to ascertain. Evidence has accumulated to suggest that the InsP 3 receptor pathway has a primary role in causing Ca 2؉ release and egg activation. However, this was recently called into question by a report implicating NO as the primary egg activator. In the present study we pursue the hypothesis that NO is a primary egg activator in sea urchin eggs and build on previous findings that an NO/cGMP/cyclic ADP-ribose (cADPR) pathway is active at fertilization in sea urchin eggs to define its role. Using a fluorescence indicator of NO levels, we have measured both NO and Ca 2؉ at fertilization and establish that NO levels rise after, not before, the Ca 2؉ wave is initiated and that this rise is Ca 2؉ -dependent. By inhibiting the increase in NO at fertilization, we find not that the Ca 2؉ transient is abolished but that the duration of the transient is significantly reduced. The latency and rise time of the transient are unaffected. This effect is mirrored by the inhibition of cGMP and cADPR signaling in sea urchin eggs at fertilization. We establish that cADPR is generated at fertilization, at a time comparable to the time of the rise in NO levels. We conclude that NO is unlikely to be a primary egg activator but, rather, acts after the initiation of the Ca 2؉ wave to regulate the duration of the fertilization Ca 2؉ transient.

show abstract

“…These commonly studied species are all deuterostomes. Stricker's excellent survey of calcium signalling across the animal kingdom (Stricker 1999) demonstrates that all eggs from protostomes tested so far (cnidarians, nemerteans, molluscs and echiurans) respond to InsP 3 , as do algal gametes (Roberts & Brownlee 1995 (Nuccitelli et al 1993), sea urchins (Crossley et al 1991;Galione et al 1993;Lee et al 1993;Lee & Shen 1998;Mohri et al 1995), starfish (Stricker 1995;Santella et al 1999a), ascidians (McDougall & Sardet 1995 and mice (Kline & Kline 1994). Only in frog (Stith et al 1993(Stith et al , 1994 and sea urchin (Ciapa & Whitaker 1986;Kuroda et al 2001) eggs has InsP 3 been shown to increase at fertilization; measurements have not been reported in other species.…”

Section: Fertilization Second Messengersmentioning

confidence: 99%

Calcium signalling in early embryos

Whitaker

2008

Phil. Trans. R. Soc. B

View full text Add to dashboard Cite

The onset of development in most species studied is triggered by one of the largest and longest calcium transients known to us. It is the most studied and best understood aspect of the calcium signals that accompany and control development. Its properties and mechanisms demonstrate what embryos are capable of and thus how the less-understood calcium signals later in development may be generated. The downstream targets of the fertilization calcium signal have also been identified, providing some pointers to the probable targets of calcium signals further on in the process of development.In one species or another, the fertilization calcium signal involves all the known calcium-releasing second messengers and many of the known calcium-signalling mechanisms. These calcium signals also usually take the form of a propagating calcium wave or waves.Fertilization causes the cell cycle to resume, and therefore fertilization signals are cell-cycle signals. In some early embryonic cell cycles, calcium signals also control the progress through each cell cycle, controlling mitosis.Studies of these early embryonic calcium-signalling mechanisms provide a background to the calcium-signalling events discussed in the articles in this issue.

show abstract

The Sperm-Induced Ca2+ Wave Following Fertilization of the Xenopus Egg Requires the Production of Ins(1,4,5)P3

Cited by 174 publications

References 0 publications

Ca²⁺ signaling differentiation during oocyte maturation

Ca²⁺ signaling differentiation during oocyte maturation

The NO Pathway Acts Late during the Fertilization Response in Sea Urchin Eggs

Calcium signalling in early embryos

Contact Info

Product

Resources

About

The Sperm-Induced Ca2+ Wave Following Fertilization of the Xenopus Egg Requires the Production of Ins(1,4,5)P3

Cited by 174 publications

References 0 publications

Ca2+ signaling differentiation during oocyte maturation

Ca2+ signaling differentiation during oocyte maturation

The NO Pathway Acts Late during the Fertilization Response in Sea Urchin Eggs

Calcium signalling in early embryos

Contact Info

Product

Resources

About

Ca²⁺ signaling differentiation during oocyte maturation

Ca²⁺ signaling differentiation during oocyte maturation