The spike glycoprotein genes of porcine epidemic diarrhea viruses isolated in China

Wang, Peihua; Li, Yaqian; Pan, Yuanqing; Guo, Yanyan; Guo, Fan; Shi, Rui-Zhu; Xing, Li

doi:10.1186/s13567-021-00954-6

Cited by 12 publications

(8 citation statements)

References 79 publications

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“…Interestingly, an immunogenicity analysis showed that rAJ1102-S2′ JS2008 induced neutralizing antibodies against both PEDV strains AJ1102 and JS2008. Epidemiological investigations have indicated that G2 variants are the predominant PEDV strains in circulation, but the classical G1 strains are still present and circulating in China and other countries (42,43). Therefore, rAJ1102-S2′ JS2008 may be an important alternative novel vaccine strain because it simultaneously provides protection against both G1 and G2 PEDV infections.…”

Section: Discussionmentioning

confidence: 99%

Construction and immunogenicity of a trypsin-independent porcine epidemic diarrhea virus variant

Zhang

Fang

et al. 2023

Front. Immunol.

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Porcine epidemic diarrhea virus (PEDV) is a re-emerging enteropathogenic coronavirus that causes high mortality in neonatal piglets. The addition of trypsin plays a crucial role in the propagation of PEDV, but also increases the complexity of vaccine production and increases its cost. Previous studies have suggested that the S2′ site and Y976/977 of the PEDV spike (S) protein might be the determinants of PEDV trypsin independence. In this study, to achieve a recombinant trypsin-independent PEDV strain, we used trypsin-dependent genotype 2 (G2) PEDV variant AJ1102 to generate three recombinant PEDVs with mutations in S (S2′ site R894G and/or Y976H). The three recombinant PEDVs were still trypsin dependent, suggesting that the S2′ site R894 and Y976 of AJ1102 S are not key sites for PEDV trypsin dependence. Therefore, we used AJ1102 and the classical trypsin-independent genotype 1 (G1) PEDV strain JS2008 to generate a recombinant PEDV carrying a chimeric S protein, and successfully obtained trypsin-independent PEDV strain rAJ1102-S2′JS2008, in which the S2 (amino acids 894–1386) domain was replaced with the corresponding JS2008 sequence. Importantly, immunization with rAJ1102-S2′JS2008 induced neutralizing antibodies against both AJ1102 and JS2008. Collectively, these results suggest that rAJ1102-S2′JS2008 is a novel vaccine candidate with significant advantages, including no trypsin requirement for viral propagation to high titers and the potential provision of protection for pigs against G1 and G2 PEDV infections.

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Section: Discussionmentioning

confidence: 99%

Construction and immunogenicity of a trypsin-independent porcine epidemic diarrhea virus variant

Zhang

Fang

et al. 2023

Front. Immunol.

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“…The antigenic epitopes inducing neutralizing antibodies against PEDV infection in pigs are mainly located in the S glycoprotein. The mutations therefore more frequently occur in the S protein of PEDV [ 40 , 41 , 42 ]. Mutations in the S protein can lead to a variety of structural and functional consequences and contribute to the lack of efficacy of PEDV vaccines [ 42 , 43 , 44 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

Antibody Evaluation and Mutations of Antigenic Epitopes in the Spike Protein of the Porcine Epidemic Diarrhea Virus from Pig Farms with Repeated Intentional Exposure (Feedback)

Thi

Chen

Chung

et al. 2022

Viruses

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The feedback strategy, or controlled exposure of pig herd to the porcine epidemic diarrhea virus (PEDV), significantly decreased losses during a severe outbreak in late 2013 in Taiwan. However, some pig farms still suffered from recurrent outbreaks. To evaluate the association between antibody titers and clinical manifestations, sera and colostra were analyzed from one pig farm that employed the feedback strategy. Furthermore, spike (S) gene full sequences from six positive samples of two farms with and without using feedback were compared to investigate the evolution of PEDV variants circulating in pig herds. The results in this study showed that high PEDV antibody titers do not correlate with the high rate of protection from PEDV infection. In addition, repeated feedback generated the emergence of PEDV variants with unique substitutions of N537S and Y561H in the COE domain and S769F in the SS6 epitopes. These mutations indicated the pathogenetic evolution of PEDV strains existing in the cycle of the feedback method. A very strict biosecurity practice to block the routes of pathogen transfer should be followed to achieve successful control of PEDV infections in pig herds.

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“…Porcine epidemic diarrhea virus (PEDV) is a novel virus that causes severe enteric disease in pigs; it belongs to the genus Alphacoronavirus [ 1 ]. The spread of PEDVs in pigs occurs via the oral–fecal route as well as through feeding and aerosol transmission [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic and Spatiotemporal Analyses of Porcine Epidemic Diarrhea Virus in Guangxi, China during 2017–2022

Bai

et al. 2023

Animals

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Since 2010, porcine epidemic diarrhea virus (PEDV) has swept across China and spread throughout the country, causing huge economic losses. In this study, 673 diarrhea samples from 143 pig farms in Guangxi during 2017–2022 were collected and detected for PEDV. Ninety-eight strains were selected for S1 gene analyses and these strains were classified into four subgroups (G1b, G2a, G2b and G2c), accounting for 1.02 (1/98), 75.51 (74/98), 16.33 (16/98) and 7.14% (7/98) of the total, respectively. Importantly, an increased number of strains in the G2c subgroup was found from 2019 onwards. Bayesian analysis revealed that Guigang may have been the epicenter of PEDVs in Guangxi. In addition, Guigang was identified as the primary hub from which PEDVs spread via two routes, namely Guigang–Wuzhou and Guigang–Laibin. Moreover, several coinfections of novel PEDV variants bearing large deletions in the partial S1 protein and PEDVs possessing an intact partial S1 protein were found in pigs. Further recombination analyses indicated that two of the strains, 18-GXNN-6 and 19-GXBH-2, originated from intra-genogroup recombination. Together, our data revealed a new profile of PEDV in Guangxi, China, which enhances our understanding of the distribution, genetic characteristics and evolutionary profile of the circulating PEDV strains in China.

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The spike glycoprotein genes of porcine epidemic diarrhea viruses isolated in China

Cited by 12 publications

References 79 publications

Construction and immunogenicity of a trypsin-independent porcine epidemic diarrhea virus variant

Construction and immunogenicity of a trypsin-independent porcine epidemic diarrhea virus variant

Antibody Evaluation and Mutations of Antigenic Epitopes in the Spike Protein of the Porcine Epidemic Diarrhea Virus from Pig Farms with Repeated Intentional Exposure (Feedback)

Phylogenetic and Spatiotemporal Analyses of Porcine Epidemic Diarrhea Virus in Guangxi, China during 2017–2022

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