The Spinach Plastid Ribosome: Protein Properties and Aspects of Ribosome Biosynthesis

Mache, Régis; Zhou, Dao‐Xiu; Franzetti, Bruno; Lagrange, Thierry; Lerbs-Mache, Silva; Bisanz-Seyer, Cordelia

doi:10.1007/978-1-4615-2407-6_53

Cited by 2 publications

(4 citation statements)

References 30 publications

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“…These elements are structurally different from what has been found in the promoter regions of two other nuclear genes rpsl and rpl21 encoding plastid ribosomal proteins [29,19]. Therefore, the rps22 gene belongs to a new class of nuclear genes different from those encoding plastid procaryotic-like ribosomal proteins.…”

Section: Discussionmentioning

confidence: 86%

“…Specifically, a cis element called S 1F binding site was identified in both the rpsl and the rp121 promoter regions that functions as a negative element repressing the rpsl promoter in root tissues [25]. Structural similarities between two different ribosomal protein genes suggest the existence of a common regulatory mechanism in nuclear genes of plastid ribosomal proteins [ 19].…”

Section: Introductionmentioning

confidence: 99%

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Cis-acting elements and expression pattern of the spinach rps22 gene coding for a plastid-specific ribosomal protein

Zhou

Clabault

et al. 1995

Plant Mol Biol

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In order to study the regulation of nuclear genes coding for plastid ribosomal proteins, we have analysed the promoter region of spinach rps22 using both in vitro and in vivo approaches. By footprinting analyses, we have identified eight DNA elements interacting with spinach leaf nuclear factors in the 300 bp promoter region upstream of the transcription start site. Among these elements, four are short AT-rich sequences and one is identical to the Hex motif characterized initially in wheat histone genes. In transgenic tobacco plants, the reporter gene coding for the beta-glucuronidase (GUS) directed by a 1.2 kb upstream region of rps22 was expressed in several plant organs, with high levels in leaf mesophyll, embryo cotyledons and root meristematic cells and very low levels in other cell types. Interestingly, when deleted to -295, the promoter, which contained all the foot-printed elements, was still able to confer the same expression pattern, although the activity was relatively lower than with the 1.2 kb promoter. When deleted further to -154, the promoter, from which the AT-rich elements were eliminated, loses its activity almost completely, suggesting that these AT-rich elements are important for the rps22 promoter activity. Altogether, our results show that rps22 gene expression is controlled by specific cis elements not present in other nuclear-encoded plastid ribosomal protein genes studied so far.

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Section: Discussionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Cis-acting elements and expression pattern of the spinach rps22 gene coding for a plastid-specific ribosomal protein

Zhou

Clabault

et al. 1995

Plant Mol Biol

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“…Number and Nomenclature Recent reviews provide an overview of chloroplast ribosomal proteins and the genes that encode them (36,377,379,606,608,609,615). For a concise summary of structure and function of individual ribosomal proteins, with emphasis on the E. coli ribosome, see the review by Liljas (358).…”

Section: Ribosomal Proteinsmentioning

confidence: 99%

“…Biogenesis of chloroplast ribosomes requires expression of both nuclear and chloroplast genes encoding different ribosomal proteins, as well as chloroplast genes encoding the component rRNAs. The mechanisms by which the appropriate stoichiometry of these components is achieved from genes present in vastly different copy numbers remain poorly understood (229,377,379). In general, nuclear gene expression in plants tends to be controlled at the level of transcription and to be subject to light regulation, whereas chloroplast gene expression is largely regulated posttranscriptionally.…”

Section: Synthesis Of the Components Of Chloroplast Ribosomesmentioning

confidence: 99%

Chloroplast ribosomes and protein synthesis.

Harris¹,

Boynton²,

Gillham³

1994

Microbiological Reviews

103

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ORIGIN OF CHLOROPLASTS Chloroplasts and mitochondria contain protein synthesizingsystems more similar to those of bacteria than to those of the eukaryotic cytoplasm, consistent with the hypothesis that these organelles had xenogenous (endosymbiotic) rather than autogenous (intracellular differentiation) origins (see. references 5, 205, 220-223, 274, 633, and 694 for discussions). Phylogenies based mostly on rRNA sequences indicate that the cyanobacteria are ancestral to chloroplasts while the members of the alpha subdivision of the purple sulfur bacteria are the likely progenitors of mitochondria (221, 222). Whether the, chlorophyte algae and land plants on the one hand, and the rhodophyte, chromophyte, and euglenoid algae on the other represent more than one endosymbiotic event remains unresolved (130, 403, 434). Comparisons of gene order and arrangement

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The Spinach Plastid Ribosome: Protein Properties and Aspects of Ribosome Biosynthesis

Cited by 2 publications

References 30 publications

Cis-acting elements and expression pattern of the spinach rps22 gene coding for a plastid-specific ribosomal protein

Cis-acting elements and expression pattern of the spinach rps22 gene coding for a plastid-specific ribosomal protein

Chloroplast ribosomes and protein synthesis.

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