The spindle position checkpoint: how to deal with spindle misalignment during asymmetric cell division in budding yeast

Fraschini, Roberta; Venturetti, Marianna; Chiroli, Elena; Piatti, Simonetta

doi:10.1042/bst0360416

Cited by 37 publications

(37 citation statements)

References 58 publications

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“…Zarzov et al observed via electron microscopy specific defects in spindle pole body (SPB) duplication which could be corrected by the overexpression of the Hsp90-encoding gene HSP82, implying a role for this chaperone in maintaining SPB function during heat shock (525). SPB defects in these mutants are also consistent with the activation of the spindle pole checkpoint and the G 2 /M arrest point (136).…”

Section: Physiological and Metabolic Adaptationmentioning

confidence: 49%

Biology of the Heat Shock Response and Protein Chaperones: Budding Yeast (Saccharomyces cerevisiae) as a Model System

Verghese

Abrams

Wang

et al. 2012

Microbiol Mol Biol Rev

503

410

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SUMMARY The eukaryotic heat shock response is an ancient and highly conserved transcriptional program that results in the immediate synthesis of a battery of cytoprotective genes in the presence of thermal and other environmental stresses. Many of these genes encode molecular chaperones, powerful protein remodelers with the capacity to shield, fold, or unfold substrates in a context-dependent manner. The budding yeast Saccharomyces cerevisiae continues to be an invaluable model for driving the discovery of regulatory features of this fundamental stress response. In addition, budding yeast has been an outstanding model system to elucidate the cell biology of protein chaperones and their organization into functional networks. In this review, we evaluate our understanding of the multifaceted response to heat shock. In addition, the chaperone complement of the cytosol is compared to those of mitochondria and the endoplasmic reticulum, organelles with their own unique protein homeostasis milieus. Finally, we examine recent advances in the understanding of the roles of protein chaperones and the heat shock response in pathogenic fungi, which is being accelerated by the wealth of information gained for budding yeast.

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Section: Physiological and Metabolic Adaptationmentioning

confidence: 49%

Biology of the Heat Shock Response and Protein Chaperones: Budding Yeast (Saccharomyces cerevisiae) as a Model System

Verghese

Abrams

Wang

et al. 2012

Microbiol Mol Biol Rev

503

410

View full text Add to dashboard Cite

show abstract

“…Cells lacking cytoplasmic dynein (dyn1D cells) frequently misposition their spindles at low temperature and arrest in anaphase because the MEN GTPase Tem1 is inhibited by Bub2-Bfa1 (for review, see Fraschini et al 2008). When the GAP is inactivated by deleting BUB2 or BFA1, cells with mispositioned spindles will not arrest in anaphase, but rather exit mitosis to produce anucleate and multinucleate cells (Supplemental Fig.…”

Section: Lte1mentioning

confidence: 99%

Cdc15 integrates Tem1 GTPase-mediated spatial signals with Polo kinase-mediated temporal cues to activate mitotic exit

Rock¹,

Amon²

2011

Genes Dev.

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In budding yeast, a Ras-like GTPase signaling cascade known as the mitotic exit network (MEN) promotes exit from mitosis. To ensure the accurate execution of mitosis, MEN activity is coordinated with other cellular events and restricted to anaphase. The MEN GTPase Tem1 has been assumed to be the central switch in MEN regulation. We show here that during an unperturbed cell cycle, restricting MEN activity to anaphase can occur in a Tem1 GTPase-independent manner. We found that the anaphase-specific activation of the MEN in the absence of Tem1 is controlled by the Polo kinase Cdc5. We further show that both Tem1 and Cdc5 are required to recruit the MEN kinase Cdc15 to spindle pole bodies, which is both necessary and sufficient to induce MEN signaling. Thus, Cdc15 functions as a coincidence detector of two essential cell cycle oscillators: the Polo kinase Cdc5 synthesis/degradation cycle and the Tem1 G-protein cycle. The Cdc15-dependent integration of these temporal (Cdc5 and Tem1 activity) and spatial (Tem1 activity) signals ensures that exit from mitosis occurs only after proper genome partitioning.

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“…The spindle position checkpoint (SPOC) is the important pathway that blocks mitotic exit and cytokinesis in case of mitotic spindle misalignment [32]. This pathway is crucial for budding yeast since the division site is determined early in the yeast cell cycle, in late G1 concomitantly with bud site selection, while the mitotic spindle is assembled after this event.…”

Section: The Complex Regulation Of the Protein Kinase Kin4 A Key Plamentioning

confidence: 99%

Protein Phosphorylation is an Important Tool to Change the Fate of Key Players in the Control of Cell Cycle Progression in Saccharomyces cerevisiae

Fraschini¹,

Raspelli²,

Cassani³

2012

Protein Phosphorylation in Human Health

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The spindle position checkpoint: how to deal with spindle misalignment during asymmetric cell division in budding yeast

Cited by 37 publications

References 58 publications

Biology of the Heat Shock Response and Protein Chaperones: Budding Yeast (Saccharomyces cerevisiae) as a Model System

Biology of the Heat Shock Response and Protein Chaperones: Budding Yeast (Saccharomyces cerevisiae) as a Model System

Cdc15 integrates Tem1 GTPase-mediated spatial signals with Polo kinase-mediated temporal cues to activate mitotic exit

Protein Phosphorylation is an Important Tool to Change the Fate of Key Players in the Control of Cell Cycle Progression in Saccharomyces cerevisiae

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