The Split Ejaculate of the Boar: Contributions of the Epididymides and Seminal Vesicles

Lavon, U.; Boursnell, J. C.

doi:10.1530/jrf.0.0420541

Cited by 19 publications

(22 citation statements)

References 26 publications

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“…Furthermore, Zn is included in the WHO manual as an indicator of prostatic function [WHO 2010] and low levels of Zn have been found in SP of infertile men [Chia et al 2000;Colagar et al 2009]. In boars, some changes in mineral composition are related to season and heat stress [Larsson and Einarsson 1984;Murase et al 2007] Although several studies are available on these enzymes and minerals in boar semen, their association with semen quality was not clearly demonstrated [Boursnell et al 1973;Ciereszko and Strzezek 1989;Einarsson et al 1970;Lavon and Boursnell 1975;Strzezek et al 1995;Strzezek et al 1998;Wysocki and Strzezek 2006]. Moreover, many of these studies are rather dated.…”

Section: Introductionmentioning

confidence: 99%

Boar seminal plasma components and their relation with semen quality

Rodriguez

Rijsselaere

Beek

et al. 2012

Systems Biology in Reproductive Medicine

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Select boar seminal plasma (SP) components and their relation to semen quality were investigated. Thirty nine boars from three artificial insemination (AI) centers were divided into group A (GA: > 80% normal sperm and >70% motility) and group B (GB: < 80% normal sperm and < 70% motility). Each ejaculate was collected and semen volume, concentration, sperm motility (computer aided semen analysis; CASA), morphology, and vitality (both eosin nigrosin staining) were investigated. The SP was separated and analyzed for aspartate-amino-transferase (AST), γ-glutamyl-transferase (GGT), alkaline phosphatase (ALP) activity, and the concentrations of sodium (Na), potassium (K), chloride (Cl), calcium (Ca), phosphate (PO 4 3-), magnesium (Mg), selenium (Se) and zinc (Zn) were assessed. Repeated measures (2 months interval) were conducted in eight boars of GA from one AI center. The activity of GGT (r = -0.482) and ALP (r = -0.459) was moderately associated ( p < 0.05) with ejaculate volume and strongly associated with concentration (r = 0.580 and r = 0.618, respectively; p = 0.000). Moderate associations ( p < 0.05) were found between ALP (r = 0.439), GGT (r = 0.387), Na (r = -0.428), K (r = 0.354), and Se (r = 0.354) with progressive motility. The SP concentration of Na (r = -0.401), Cl (r = -0.521), and K (r = 0.350) was associated ( p < 0.05) with normal morphology. Only Mg was associated ( p < 0.05) with membrane damage (r = -0.335). The concentration of Na, Cl, and Zn (1681.0 vs. 1701.0 µg/dL) was different between groups ( p < 0.05). Repeated measures showed significant differences in time but only for Na, Mg, and Zn ( p < 0.05). In conclusion, several biochemical components of SP were related to semen quality. The analysis of biochemical parameters could provide extra information about reproductive health of AI boars.

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Section: Introductionmentioning

confidence: 99%

Boar seminal plasma components and their relation with semen quality

Rodriguez

Rijsselaere

Beek

et al. 2012

Systems Biology in Reproductive Medicine

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“…Testicular firmness ( (Lavon and Boursnell 1975) and absorbed by spermatozoa (Boursnell and Nobel 1975 For personal use only.…”

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confidence: 99%

The Effect of a 15-H Photoperiod on Reproductive Function in Boars at 2, 3, 4 or 5 Months of Age

Greenberg¹,

Mahone²

1981

Can. J. Anim. Sci.

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GnrrNnrnc, L. G. nNo ManoNs, J. P. I 981 . The effect of a 15-h photoperiod on reproductivefunctioninboards at2,3,4 or5monthsof age. Can. J. Anim. Sci. 6l:925-934.The effects of supplemental lighting in stimulating reproductive function in prepuberal boius were studied using littermate boars divided equally into four groups (n : 9). All boarsreceivednatural daylighr(9.2 12.8h)until 2moofage,followedbyarrificiallight given daily according to the following schedule: boars wereexposed to a 15-hphotoperiod beginning at the age ot 2 (group I); 3 (group II); 4 (group III) or 5 mo (group IV). Libido scores of the four groups increased with age (P < 0.001 ). Seminal plasma protein (P < 0.01) and zinc concentration (P < 0.05), sperm-rich concentration, sperm-rich volume, total semen volume and total viable sperm increased (P < 0.05) with age in all four groups. The photoperiod treatment had no effect on semen quality on a per ejaculate basis between the groups; 15 h of light per day was not effective in accelerating the onset of puberty when administered from 2, 3, 4 or 5 mo of age onward.Dans cette 6tude sur des verrats pr6pub6raux, l'utilisation d'un 6clairage prolong6 d 6t6 6valu6e comme moyen de stimuler leur efficacit6 de reproduction. Chacun des groupes avait la m6me composition quant i l'origine maternelle des verats. Le programme d'dclairagea6t6 lesuivant: naturel (9.2-12.8h) jusqu'dl'dgede2mois, l5hdelumidre

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“…Caudal spermatozoa are sequentially released during ejaculation and mixed in the urethra with secretions of the various accessory sex glands to form the ejaculate (Marberger 1974;Lavon and Boursnell 1975). The ejaculate is constituted of two parts: spermatozoa and SP, the latter building its volume (95%, Mann and Lutwak-Mann 1981).…”

Section: The Boar Ejaculatementioning

confidence: 99%

Sperm Membrane Channels, Receptors and Kinematics : Using boar spermatozoa for drug toxicity screening

Vicente-Carrillo¹

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About the cover:The figure in the front cover displays boar spermatozoa where AQP-9 (upper-left corner), CatSper 3 (upper-right corner) and the δ-opioid receptor (lower-left corner) are immunolocalized. A representative output of the Qualisperm™ software depicting each detected spermatozoon and its motility trajectory (in yellow) is provided in the lower-right corner.During the course of the research underlying this Thesis, Alejandro Vicente Carrillo was enrolled in Forum Scientium, a multidisciplinary doctoral program at Linköping University. Printed by LiU-Tryck, Linköping, Sweden, 2016 To my family MAIN SUPERVISOR Heriberto Rodríguez-Martínez ABSTRACTInternal fertilization usually implies that a spermatozoon, with intact attributes for zygote formation, passes all hurdles during its transport through the female genitalia and reaches the oocyte. During this journey, millions to billions of other spermatozoa perish. Spermatozoa are highly differentiated motile cells without synthetic capabilities. They generate energy via glycolysis and oxidative phosphorylation to sustain motility and to maintain the stability and functionality of their plasma membrane. In vivo, they spend their short lifespan bathing in female genital tract fluids of different origins, or are in vitro exposed to defined media during diverse sperm handling i.e. extension, cryopreservation, in vitro fertilization, etc. Being excitable cells, spermatozoa respond in vivo to various stimuli during pre-fertilization (capacitation, hyperactivation, oocyte location) and fertilization (acrosome reaction, interaction with the oocyte) events, mediated via diverse membrane ion-conducting channels and ligand-gated receptors. The present Thesis has mapped the presence and reactivity (sperm intactness and kinematics) of selected receptors, water and ion channels in ejaculated boar spermatozoa. The final aim was to find a relevant alternative cell type for in vitro bioassays that could ease the early scrutiny of candidate drugs as well as decreasing our needs for experimental animals according to the 3R principles. Spermatozoa are often extended, cooled and thawed to warrant their availability as fertile gametes for breeding or in vitro testing. Such manipulations stress the cells via osmotic variations and hence spermatozoa need to maintain membrane intactness by controlling the exchange of water and the common cryoprotectant glycerol, via aquaporins (AQPs). Both AQPs-7 and -9 were studied for membrane domain changes in cauda-and ejaculated spermatozoa (un-processed, extended, chilled or frozen-thawed). While AQP-9 maintained location through source and handling, thawing of ejaculated spermatozoa clearly relocated the labelling of AQP-7, thus appearing as a relevant marker for non-empirical studies of sperm cryopreservation. Alongside water, spermatozoa interact with calcium (Ca 2+ ) via the main Ca 2+ sperm channel CatSper. Increments in intracellular Ca 2+ initiate motility hyperactivation and the acrosome reaction. The four subunits of the CatSper ch...

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The Split Ejaculate of the Boar: Contributions of the Epididymides and Seminal Vesicles

Cited by 19 publications

References 26 publications

Boar seminal plasma components and their relation with semen quality

Boar seminal plasma components and their relation with semen quality

The Effect of a 15-H Photoperiod on Reproductive Function in Boars at 2, 3, 4 or 5 Months of Age

Sperm Membrane Channels, Receptors and Kinematics : Using boar spermatozoa for drug toxicity screening

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