The sqt-1 gene of C. elegans encodes a collagen critical for organismal morphogenesis

Kramer, James M.; Johnson, James E.; Edgar, R. S.; Basch, Corrine; Roberts, Stephen

doi:10.1016/0092-8674(88)90214-0

Cited by 113 publications

(60 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We selected a series of overlapping cosmid clones (Coulson et al, 1986(Coulson et al, , 1988 covering this region of the genome for analysis. Because genetic data suggested dpy-2 and dpy-10 might encode collagens, we probed DNAs from these cosmids with a clone containing the sqt-1 collagen gene (Kramer et al, 1988). Cross-hybridization between most collagen genes occurs under low stringency hybridization conditions because of the shared Gly-X-Y encoding sequences common to all collagens.…”

Section: Resultsmentioning

confidence: 99%

“…Subclones from cosmid ZK857 were constructed through a "shotgun" approach by digesting cosmid DNA with HindIII, ligating into HindIII-digested pBS plasmid (Stratagene, Lajolla, CA), and transforming into JM109 Escherichia coli cells. Colonies were probed with sqt-1-containing clones (Kramer et al, 1988) under low stringency, yielding plasmids pALl and pAL5. These plasmid subclones were nick-translated and probed to blots containing EcoRI digested genomic DNAs from strains JK1012, DR1048, and DR1049.…”

Section: Materials and Methods C Elegans Strains And Geneticsmentioning

confidence: 99%

“…elegans contains between 50 and 150 collagen genes (Kramer et al, 1982;Cox et al, 1984Cox et al, , 1985. Over 30 of these genes have been isolated and, except for the two type IV collagen genes (Guo and Kramer, 1989), each has been shown to encode an '30-kDa product that contains several short interruptions of the Gly-X-Y repeat domain (Kramer et al, 1982(Kramer et al, , 1988(Kramer et al, , 1990von Mende et al, 1988;Cox et al, 1989;Bird, 1992;Johnstone et al, 1993). It is likely that most, or all, of these small collagens are localized to the cuticle.…”

Section: Introductionmentioning

confidence: 99%

“…These abnormal morphologies fall into five classes: dumpy (short and fat), roller (helically twisted), long, squat (dominant roller alleles and recessive dumpy alleles), and blister (blisters on cuticle). Four such genes that affect organismal morphology have been isolated and shown to encode collagens: sqt-1 (Kramer et al, 1988), dpy-13 (von Mende et al, 1988), rol-6 (Kramer et al, 1990), and dpy-7 aohnstone et al, 1993). Defective collagens are therefore able to cause these morphologic defects.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Molecular and genetic analyses of the Caenorhabditis elegans dpy-2 and dpy-10 collagen genes: a variety of molecular alterations affect organismal morphology.

Levy

Yang

Kramer

1993

MBoC

View full text Add to dashboard Cite

We have identified and cloned the Caenorhabditis elegans dpy-2 and dpy-10 genes and determined that they encode collagens. Genetic data suggested that these genes are important in morphogenesis and possibly other developmental events. These data include the morphologic phenotypes exhibited by mutants, unusual genetic interactions with the sqt-1 collagen gene, and suppression of mutations in the glp-1 and mup-i genes. The proximity of the dpy-2 and dpy-10 genes (3.5 kilobase) and the structural similarity of their encoded proteins (41% amino acid identity) indicate that dpy-2 and dpy-10 are the result of a gene duplication event. The genes do not, however, appear to be functionally redundant, because a dpy-10 null mutant is not rescued by the dpy-2 gene. In addition, full complementation between dpy-2 and dpy-10 can be demonstrated with all recessive alleles tested in trans. Sequence analysis of several mutant alleles of each gene was performed to determine the nature of the molecular defects that can cause the morphologic phenotypes. Glycine substitutions within the Gly-X-Y portion of the collagens can result in dumpy (Dpy), dumpy, left roller (DLRol), or temperature-sensitive DLRol phenotypes. dpy-1O(cn64), a dominant temperature-sensitive DLRol allele, creates an Arg-to-Cys substitution in the amino non-Gly-X-Y portion of the protein. Three dpy-10 alleles contain Tcl insertions in the coding region of the gene. dpy-lO(cg36) (DLRol) creates a nonsense codon near the end of the Gly-X-Y region. The nature of this mutation, combined with genetic data, indicates that DLRol is the null phenotype of dpy-10. The Dpy phenotype results from reduced function of the dpy-1O collagen gene. Our results indicate that a variety of molecular defects in these collagens can result in severe morphologic changes in C. elegans.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Materials and Methods C Elegans Strains And Geneticsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular and genetic analyses of the Caenorhabditis elegans dpy-2 and dpy-10 collagen genes: a variety of molecular alterations affect organismal morphology.

Levy

Yang

Kramer

1993

MBoC

View full text Add to dashboard Cite

show abstract

“…In this sense, bFtz-F1 has been shown to render Drosophila tissues competent to undergo changes in response to the ecdysteroid signal (Broadus et al 1999). The posterior end defects possibly result from faulty expression of cuticle constituents, such as the sqt-1 and rol-6 collagen genes (Kramer et al 1988(Kramer et al , 1990Park & Kramer 1994). …”

Section: Roles Of Nhr-25 In Post-embryonic Development and Reproductionmentioning

confidence: 99%

The conserved nuclear receptor Ftz‐F1 is required for embryogenesis, moulting and reproduction in Caenorhabditis elegans

Ishihara²,

et al. 2000

View full text Add to dashboard Cite

Background: Nuclear receptors are essential players in the development of all metazoans. The nematode Caenorhabditis elegans possesses more than 200 putative nuclear receptor genes, several times more than the number known in any other organism. Very few of these transcription factors are conserved with components of the steroid response pathways in vertebrates and arthropods. Ftz-F1, one of the evolutionarily oldest nuclear receptor types, is required for steroidogenesis and sexual differentiation in mice and for segmentation and metamorphosis in Drosophila.

show abstract

Cuticle chirality and body handedness inCaenorhabditis elegans

et al. 1998

Self Cite

View full text Add to dashboard Cite

The sqt-1 gene of C. elegans encodes a collagen critical for organismal morphogenesis

Cited by 113 publications

References 47 publications

Molecular and genetic analyses of the Caenorhabditis elegans dpy-2 and dpy-10 collagen genes: a variety of molecular alterations affect organismal morphology.

Molecular and genetic analyses of the Caenorhabditis elegans dpy-2 and dpy-10 collagen genes: a variety of molecular alterations affect organismal morphology.

The conserved nuclear receptor Ftz‐F1 is required for embryogenesis, moulting and reproduction in Caenorhabditis elegans

Cuticle chirality and body handedness inCaenorhabditis elegans

Contact Info

Product

Resources

About