The stability investigation of compound Danshen injection (a traditional medicine) with a new high-performance liquid chromatography method

Abstract

Background:Compound Danshen injection (CDSI, a traditional medicine) is an effective drug for the treatment of cardiovascular and cerebrovascular diseases. However, the research about its stability is absent.Objective:A new high-performance liquid chromatography method was developed to assay its main effective constituents, i.e., propanoid acid (PA), protocatechuic aldehyde (PHA), salvianolic acid B (SAB), salvianolic acid A (SAA), and rosmarinic acid (RA). Through the newly found method, the stability of CDSI was to be investigated.Materials and Methods:The analysis was performed by a reverse-phase gradient elution using an aqueous mobile phase (containing 0.1% acetic acid) modified by acetonitrile, and detection was made simultaneously at 280 nm and 325 nm. The method was validated for accuracy, precision and limits of detection. The effects of some environmental storage conditions (light and temperature) on the stability of CDSI were investigated.Results:This method is precise, simple, and convenient. The result showed that illumination and temperature had an obvious effect on CDSI's stability. SAA is the most unstable one among the five components. In the condition of common light, it decomposed rapidly to almost 50% after only 4 h, and 100% after 8 h. PA, RA, and PHA might come from Danshen, was also the transformed products from other components in store process.Conclusion:The result indicated that the main active constituents in CDSI suffered from the illumination and temperature greatly. CDSI should be stored at low temperature and kept away from light.

Extraction Kinetics of Phenolic Antioxidants from the Hydro Distillation Residues of Rosemary and Effect of Pretreatment and Extraction Parameters

“…The spectrum of the compound exhibits a pattern detected in specific members of caffeic acid oligomers, such as salvianolic acids. The pattern matches salvianolic acid A, in agreement with the already reported corresponding spectra [ 52 , 53 ]. The ethanol and ethanol-water extractions resulted in similar peak patterns to acetone and acetone-water extracts.…”

Activation of the AMPK-SIRT1 pathway contributes to protective effects of Salvianolic acid A against lipotoxicity in hepatocytes and NAFLD in mice

“…Although there is no evidence of clinical application of salvianolic acid A alone, Salvianolic acids for injection (SAI) is a clinical used medicinal preparation composed of multiple salvianolic acids from the aqueous extracts of Danshen. The concentration of Sal A in SAI is 72 μg/ml (Zhang et al, 2013). We estimated that the concentration of Sal A in the blood of a human body is about 0.65 μM with a recommended dosage of 20 ml/d via intravenous injection.…”