The Stability of Parathyroid Hormone in Blood and Serum Samples at 4°C and at Room Temperature

Russell, Daniel H; Henley, R

doi:10.1177/000456329503200217

Cited by 9 publications

(11 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ratcli¡e et al 3 observed decreases of 17% in serum PTH concentration over 24 h and 32% in whole blood samples using an Incstar assay. Russell and Henley, 4 using Ciba-Corning Magiclite, found PTH to be stable in both whole blood SST and in serum for up to 6 h at room temperature. However, samples stored at room temperature for 24^30 h showed signi¢cant decreases in measured PTH, with whole blood and serum concentrations falling by 25% and 30%, respectively.…”

Section: Discussionmentioning

confidence: 99%

Stability of parathyroid hormone ex vivo in haemodialysis patients

et al. 2003

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Stability of parathyroid hormone ex vivo in haemodialysis patients

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Fifteen papers relating to PTH stability in whole blood were identified that had used a second generation assay ( Figure 2, Supplemental Data Table 3) [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29]. Collation of study results was difficult due to variation in experimental design (see above), but almost all studies suggested that PTH was more stable in whole blood samples containing EDTA than in anticoagulant-free samples (Supplemental Data Table 3).…”

Section: Stability In Anticoagulated and Clotted Whole Blood At Room mentioning

confidence: 99%

“…Table 3). Nine studies addressed stability at 4°C in serum or plasma (Supplemental Data Table 4) [19,22,24,25,29,33,34,38,39]. Five of these directly compared the stability of PTH in serum and plasma at 4°C [25,29,33,34,38] using second generation assays generally confirming superior stability in EDTA (Table 3).…”

Section: Stability At Other Temperaturesmentioning

confidence: 99%

Sampling and storage conditions influencing the measurement of parathyroid hormone in blood samples: a systematic review

Hanon

Sturgeon

Lamb

2013

Clinical Chemistry and Laboratory Medicine (CCLM)

View full text Add to dashboard Cite

Parathyroid hormone (PTH) is relatively unstable: optimisation of pre-analytical conditions, including specimen type, sampling time and storage conditions, is essential. We have undertaken a systematic review of these pre-analytical conditions. An electronic search of the PubMed, Embase, Cochrane, Centre for Research and Dissemination and Bandolier databases was undertaken. Of 5511 papers identified, 96 underwent full text review, of which 83 were finally included. At room temperature PTH was stable in ethylenediaminetetraacetic acid (EDTA) preserved whole blood for at least 24 h and in EDTA plasma for at least 48 h after venepuncture. Losses were observed in clotted blood samples after 3 h and in serum after 2 h. At 4°C PTH was more stable in EDTA plasma (at least 72 h) than serum (at least 24 h). Central venous PTH concentrations were higher than peripheral venous concentrations. In the northern hemisphere, PTH concentrations were higher in winter than summer. PTH has a circadian rhythm characterised by a nocturnal acrophase and mid-morning nadir. Data related to frozen storage of PTH (−20°C and −80°C) were limited and contradictory. We recommend that blood samples for PTH measurement should be taken into tubes containing EDTA, ideally between 10:00 and 16:00, and plasma separated within 24 h of venepuncture. Plasma samples should be stored at 4°C and analysed within 72 h of venepuncture. Particular regard must be paid to the venepuncture site when interpreting PTH concentration. Further research is required to clarify the suitability of freezing samples prior to PTH measurement.

show abstract

“…PTH concentrations in serum specimens are reduced after incubating at room temperature for more than a few hours [3][4][5], probably due to proteolytic cleavage by proteases.…”

mentioning

confidence: 99%

The Comparison of Parathyroid Hormone Degradation Effect by Various Protease Inhibitors in Blood Specimen

2009

View full text Add to dashboard Cite

Parathyroid hormone (PTH) is released from the parathyroid glands and regulates bone and mineral metabolism. It is a single-chain 84-amino acid polypeptide with a molecular weight of 9,425 Daltons. It is synthesized and secreted by the parathyroid glands, and rapidly cleared from plasma (half-life <5 min). PTH is metabolized to C-terminal fragments by the liver and kidneys, and cleared by the kidney, either as intact PTH or as C-terminal fragments [1]. Background : The objective of this study was to evaluate the role of proteases on the degradation of parathyroid hormone (PTH) in blood samples.Methods : Protease inhibitors with specificity against serine proteases (aprotinin), cysteine proteases (E-64), serine and cysteine proteases (leupeptin), metalloproteases (EDTA), or a protease inhibitor cocktail with a broad spectrum of inhibitory activity were added to blood samples. After storage at room temperature (0-48 hr), PTH levels were measured.Results : PTH levels in samples with the protease inhibitor cocktail did not change significantly after 48 hr of storage at room temperature, but the average PTH levels decreased by 40.7% and 20.1%, in samples stored at room temperature and stored at 4°C without protease inhibitors, respectively. PTH levels in samples with leupeptin were stable for up to 24 hr. After 48 hr, the mean PTH levels decreased by 17.1%, 16.0%, 26.2%, and 32.1%, with 500 KIU/mL aprotinin, 100 μ mol/L leupeptin, 10 μ mol/L E-64, and 10 μ mol/L EDTA, respectively, in the samples stored at room temperature.Conclusions : The decrease in PTH levels in blood samples seemed to be due to the degradation of PTH by proteases. Various proteases, including especially serine proteases, would act together to degrade PTH in blood specimen. The PTH degradation may be inhibited in blood specimen with protease inhibitor cocktail. (Korean J Lab Med 2009;29:104-9)

show abstract

The Stability of Parathyroid Hormone in Blood and Serum Samples at 4°C and at Room Temperature

Cited by 9 publications

References 2 publications

Stability of parathyroid hormone ex vivo in haemodialysis patients

Stability of parathyroid hormone ex vivo in haemodialysis patients

Sampling and storage conditions influencing the measurement of parathyroid hormone in blood samples: a systematic review

The Comparison of Parathyroid Hormone Degradation Effect by Various Protease Inhibitors in Blood Specimen

Contact Info

Product

Resources

About