The stabilization and release performances of curcumin-loaded liposomes coated by high and low molecular weight chitosan

Abstract

A comprehensive stability evaluation for curcumin-loaded liposomes (Cur-LP) coated by low (LCS) or high (HCS) molecular weight chitosan with three gradient concentrations (L: low; M: medium; H: high) was the main objective of this study. Apart from leading to a higher encapsulation efficiency (> 90%), all chitosan-coated Cur-LP displayed an improved stability with respect to resistant to salt, sunlight, heat, accelerated centrifugation and long-term storage at 4 °C. Increasing the molecular weight and concentration of chitosan could effectively improve the stability of Cur-LP, in which HCS-H coatings displayed the best performance. According to the fluorescence probe analysis, the mechanical reinforcement of liposomes and the concomitant reduction in membrane fluidity accounts for the major contribution to vesicle stability. Secondly, a simulated digestion model was used to prove the applicability of sustained curcumin release, achieved by adjusting the molecular weight and concentration of the chitosan stabilizer for Cur-LP. The results of this study show that high molecular weight chitosan used at relatively high concentrations, is a promising coating material for improving the stability and sustained release of Cur-LP in vitro.

Investigating the Anti-Inflammatory Activity of Curcumin-Loaded Silica-Containing Redox Nanoparticles

“…Tai et al (2019) recently formulated curcumin-loaded liposomal nanoparticles (Cur-LP) with the same objective. In order to improve drug bioavailability, the research coated liposome nanoparticles with high molecular weight chitosan to enhance stability and maintain the release capacity of Cur-LP [12]. Nevertheless, various designed nanoparticles shared common concerns: they exhibited low stability, whereas the release profile was inconsistent and uncontrollable.…”

Preparing, optimising, and evaluating chitosan nanocapsules to improve the stability of anthocyanins from Aronia melanocarpa

“…5A shows that there was no obvious release from both free ANT and ANT-NPs during mouth digestion, which may be due to the short exposure process and the absence of some specic enzymes. 33 The retention rate of the ANT (96.24%) in ANT-NPs was clearly higher than that of free ANT (88.21%) when the samples were subjected to gastric digestion for 1 h. The retention rate of the ANT-NPs was more than 92%, while that of the free ANT was only 76% at the end of the gastric digestion period, which is signicantly lower than those obtained from the ANT-NPs. Moreover, the retention rate of free ANT decreased rapidly to 38.52%, while that of ANT-NPs was 79.13% aer simulated intestinal digestion.…”

Polymer–Lipid Pharmaceutical Nanocarriers: Innovations by New Formulations and Production Technologies

“…To date, liposome polymeric coating is carried out through conventional methods such as the simple incubation of a liposomal suspension with a polymeric solution [ 74 , 75 ] or through the dropwise method, which in the early 1990s was explored for the coating of liposomes with chitosan [ 76 ], is still the most popular form for the production of these carrier systems. The strategy involves the dropwise addition of a polymeric solution, through the use of a syringe pump, to a preformed liposomal suspension, while stirring [ 77 ], or, on the contrary, the polymeric solution is added drop by drop to a previously produced liposome solution [ 78 ].…”

“…Over the years, the technique has led to the production of coated liposomes, mainly of the chitosan polymer, for the delivery of various active molecules such as indomethacin [ 79 ], clotrimazole [ 80 ], resveratrol [ 81 ], doxorubicin [ 56 , 82 ] quercetin [ 83 ], proteins [ 84 , 85 ], curcumin [ 78 ], and other therapeutics [ 86 , 87 ], but the fabrication processes have remained at the bench-scale, leading to the production of restricted volumes in output. In addition to the low productivity, being a bulk technique, it is also characterized by a poor control over the final features of the covered lipid vesicles and requires high energy and long periods of time for process.…”