The Stable Level of Glutamine synthetase 2 Plays an Important Role in Rice Growth and in Carbon-Nitrogen Metabolic Balance

Bao, Ande; Zhao, Zhong-Qiu; Ding, Guangda; Shi, Lei; Xu, Fangsen; Cai, Hongmei

doi:10.3390/ijms160612713

Cited by 57 publications

(43 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous study has found that transgenic rice overexpressing a chloroplastic GS gene showed enhanced tolerance to salt stress (Hoshida et al 2000). Moreover, a recent study has revealed that stable level of chloroplastic GS plays an important role in rice growth and carbon-nitrogen metabolic balance (Bao et al 2015). Therefore, it could be postulated that proline supply has increased the cellular proline concentration and subsequently affected the interregulation of plant metabolism, which was certainly important in relation to plant stress tolerance.…”

Section: Discussionmentioning

confidence: 99%

Exogenous proline significantly affects the plant growth and nitrogen assimilation enzymes activities in rice (Oryza sativa) under salt stress

Teh

Shaharuddin

et al. 2016

Acta Physiol Plant

View full text Add to dashboard Cite

Salinity has been shown to be a major factor contributing to low nitrogen availability in plants. To verify the changes in nitrogen metabolism activity as affected by the exogenous application of proline under salt stress and its relation to salt tolerance, in vitro rice shoot apices were used as a model to study the growth performance and changes in nitrogen assimilation activities in two Malaysian rice cultivars MR 220 and MR 253. Results revealed that salt stress greatly reduced the plant height, shoot nitrate (NO 3 -) content, shoot glutamine synthetase (GS), and root nitrate reductase (NR) activities in both cultivars. Supplementation of proline significantly increased the plant height, number of roots, root NO 3 -content, root NR, and root GS activities under salt stress in both cultivars with greater enhancement in MR 253 than MR 220. The results also indicated that MR 253 possessed higher nitrite reductase (NiR) and glutamate synthase (NADH-GOGAT) activities as compared with MR 220 in all tested treatments. It was suggested that the NO 3 -content, NR, and GS activities played important roles in regulating nitrogen metabolism under salt stress. Taken together, it was concluded that the ability of proline in

show abstract

Section: Discussionmentioning

confidence: 99%

Exogenous proline significantly affects the plant growth and nitrogen assimilation enzymes activities in rice (Oryza sativa) under salt stress

Teh

Shaharuddin

et al. 2016

Acta Physiol Plant

View full text Add to dashboard Cite

show abstract

“…4 Carbohydrates also serve as carbon skeletons and a source of energy for plants to assimilate ammonium (NH 4 + ). 5…”

Section: Introductionmentioning

confidence: 99%

Effect of ammonium and nitrate supplies on nitrogen and sucrose metabolism of Cabernet Sauvignon (Vitis vinifera cv.)

Yin

Wang

et al. 2020

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUND: The quality of wine is highly dependent on the quality of berries. Development of berries is influenced by the type and ratio of different nitrogen supplies in the soil. To understand the impact of varying sources and levels of nitrate and ammonium on sucrose and nitrogen metabolism of Vitis vinifera cv. Cabernet Sauvignon, we tested nutrient solutions with four NO 3 −-N:NH 4 +-N ratios (100:0, 75:25, 50:50, 0:100) through the root system. RESULTS: The form and quantity of nitrogen affected berries and leaves with source-sink relationships. Soluble sugar levels were significantly higher in plants treated with mixed nitrogen sources (75:25 and 50:50) compared to single nitrogen sources (100:0 and 0:100). In particular, treating plants with mixed nitrogen source at a 75:25 ratio resulted in 22% higher fructose levels in berries compared to the 50:50 treatment. In addition, mixed nitrogen treatments resulted in significantly higher amino acid levels and protein content. Mixed nitrogen substrates also increased the expression of enzymes involved in both nitrogen and sucrose metabolism. CONCLUSION: Plants did not maximize the nitrogen supply when single form nitrogen was provided, and the mixed nitrogen substrates consistently increased the amount of available carbon and nitrogen in the berries and leaves. We found that NO 3 − N: NH 4 +-N ratio of 75:25 was the optimum formula for improving nitrogen and sucrose metabolism, and reducing the competition between nitrogen and sucrose. By examining the nutrient utilization of plants cultivated with different nitrogen forms, the present study provides insights into improving cultivation and production practices.

show abstract

“…Amino acids, glycosides, polysaccharides, and organic acids are contained in many biostimulants and they may act as precursors or activators of endogenous plant hormones [20]. Abscisic acid (ABA) is involved in embryogenesis, seed physiology reactions, plant growth, and biotic and abiotic stress tolerance [36]. Young tissues have high ABA levels, although ABA has historically been considered a growth inhibitor [36,37].…”

Section: Discussionmentioning

confidence: 99%

“…Abscisic acid (ABA) is involved in embryogenesis, seed physiology reactions, plant growth, and biotic and abiotic stress tolerance [36]. Young tissues have high ABA levels, although ABA has historically been considered a growth inhibitor [36,37]. It has been shown that ABA-deficient mutant plants are severely stunted and conversely exogenous ABA treatment of mutants restores normal cell expansion and growth [37].…”

Section: Discussionmentioning

confidence: 99%

Expression Profiling of Candidate Genes in Sugar Beet Leaves Treated with Leonardite-Based Biostimulant

et al. 2019

View full text Add to dashboard Cite

Leonardite-based biostimulants are a large class of compounds, including humic acid substances. Foliar application of biostimulants at field level improves plant growth, yield and quality through metabolic changes and stimulation of plant proton pumps. The present study aimed at identifying optimum dosage of BLACKJAK, a humic acid-based substance, which is able to modify genes involved in sugar beet growth. Thirty-three genes belonging to various biochemical pathway categories were tested in leaves of treated sugar beet (Beta vulgaris L.) samples to assess gene expression profiling in response to BLACKJAK. Seedlings of a diploid and multigerm variety were grown in plastic pots and sprayed with two dilutions of BLACKJAK (dilution 1:500–1.0 mg C L−1 and dilution 1:1000–0.5 mg C L−1). Leaf samples were collected after 24, 48, and 72 h treatment with BLACKJAK for each dilution. RNA was extracted and the quantification of gene expression was performed while using an OpenArray platform. Results of analysis of variance demonstrated that, 15 genes out of a total of 33 genes tested with OpenArray qPCR were significantly affected by treatment and exposure time. Analysis for annotation of gene products and pathways revealed that genes belonging to the mitochondrial respiratory pathways, nitrogen and hormone metabolisms, and nutrient uptake were up-regulated in the BLACKJAK treated samples. Among the up-regulated genes, Bv_PHT2;1 and Bv_GLN1 expression exerted a 2-fold change in 1:1000 and 1:500 BLACKJAK concentrations. Overall, the gene expression data in the BLACKJAK treated leaves demonstrated the induction of plant growth–related genes that were contributed almost to amino acid and nitrogen metabolism, plant defense system, and plant growth.

show abstract

The Stable Level of Glutamine synthetase 2 Plays an Important Role in Rice Growth and in Carbon-Nitrogen Metabolic Balance

Cited by 57 publications

References 61 publications

Exogenous proline significantly affects the plant growth and nitrogen assimilation enzymes activities in rice (Oryza sativa) under salt stress

Exogenous proline significantly affects the plant growth and nitrogen assimilation enzymes activities in rice (Oryza sativa) under salt stress

Effect of ammonium and nitrate supplies on nitrogen and sucrose metabolism of Cabernet Sauvignon (Vitis vinifera cv.)

Expression Profiling of Candidate Genes in Sugar Beet Leaves Treated with Leonardite-Based Biostimulant

Contact Info

Product

Resources

About