The standardized extract of Loeselia mexicana possesses anxiolytic activity through the γ-amino butyric acid mechanism

Herrera-Ruíz, Maribel; González-Carranza, Adolfo; Zamilpa, Alejandro; Jiménez-Ferrer, Enrique; Huerta-Reyes, Maira; Navarro-García, Víctor Manuel

doi:10.1016/j.jep.2011.09.010

Cited by 22 publications

(10 citation statements)

References 45 publications

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“…The open field test was conducted after the aforementioned behavioral tests, allowing us to exclude possible nonspecific locomotor effects of antagonists or FAT treatments. In the open field test, we found that none of the GABA A antagonists alone or the FAT-M at the doses tested produced any significant changes on motor activity, similar to previous reports [4, 11, 13, 35]. Additionally, the dose administered of bicuculline and picrotoxin did not produce behaviour linked to seizures (i.e., facial/ear twitching, myoclonic jerks, among others), which are detected with higher doses than those used in the present study.…”

Section: Discussionsupporting

confidence: 92%

Participation ofGABAAChloride Channels in the Anxiolytic-Like Effects of a Fatty Acid Mixture

Rodríguez‐Landa

García-Ríos

Cueto‐Escobedo

et al. 2013

BioMed Research International

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Human amniotic fluid and a mixture of eight fatty acids (FAT-M) identified in this maternal fluid (C12:0, lauric acid, 0.9 μg%; C14:0, myristic acid, 6.9 μg%; C16:0, palmitic acid, 35.3 μg%; C16:1, palmitoleic acid, 16.4 μg%; C18:0, stearic acid, 8.5 μg%; C18:1cis, oleic acid, 18.4 μg%; C18:1trans, elaidic acid, 3.5 μg%; C18:2, linoleic acid, 10.1 μg%) produce anxiolytic-like effects that are comparable to diazepam in Wistar rats, suggesting the involvement of γ-aminobutyric acid-A (GABAA) receptors, a possibility not yet explored. Wistar rats were subjected to the defensive burying test, elevated plus maze, and open field test. In different groups, three GABAA receptor antagonists were administered 30 min before FAT-M administration, including the competitive GABA binding antagonist bicuculline (1 mg/kg), GABAA benzodiazepine antagonist flumazenil (5 mg/kg), and noncompetitive GABAA chloride channel antagonist picrotoxin (1 mg/kg). The FAT-M exerted anxiolytic-like effects in the defensive burying test and elevated plus maze, without affecting locomotor activity in the open field test. The GABAA antagonists alone did not produce significant changes in the behavioral tests. Picrotoxin but not bicuculline or flumazenil blocked the anxiolytic-like effect of the FAT-M. Based on the specific blocking action of picrotoxin on the effects of the FAT-M, we conclude that the FAT-M exerted its anxiolytic-like effects through GABAA receptor chloride channels.

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Section: Discussionsupporting

confidence: 92%

Participation ofGABAAChloride Channels in the Anxiolytic-Like Effects of a Fatty Acid Mixture

Rodríguez‐Landa

García-Ríos

Cueto‐Escobedo

et al. 2013

BioMed Research International

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“…In this animal model, rats or mice that display anxiety-like behavior usually show a reduction of open-arm exploration, reflected by a decrease in the time spent in the open arms. Animals treated with clinically effective anxiolytic drugs (e.g., diazepam) [34–36], some neurosteroids with anxiolytic-like potency (e.g., progesterone and allopregnanolone) [37–39], and some extracts from medicinal plants with reputed anxiolytic properties exhibit an increase in the total time spent in the open arms [40, 41], which is considered an anxiolytic-like effect at the experimental level.…”

Section: Discussionmentioning

confidence: 99%

Montanoa frutescensandMontanoa grandifloraExtracts Reduce Anxiety-Like Behavior during the Metestrus-Diestrus Phase of the Ovarian Cycle in Wistar Rats

Rodríguez‐Landa

Vicente-Serna

Rodríguez-Blanco

et al. 2014

BioMed Research International

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In previous studies, the anxiolytic-like effects of Montanoa tomentosa and Montanoa frutescens were reported in male rats, but the potential anxiolytic-like effects of Montanoa plants during the different phases of the ovarian cycle in rats remain to be explored. The anxiolytic-like effects of the aqueous crude extracts of M. frutescens (25 and 50 mg/kg) and M. grandiflora (25 and 50 mg/kg) in the elevated plus maze were investigated in Wistar rats during the estrous cycle and compared with 2 mg/kg diazepam as a reference anxiolytic drug. To investigate any motor effect (i.e., hyperactivity, no changes, or hypoactivity) associated with the treatments, the rats were evaluated in the open field test. The M. frutescens (25 and 50 mg/kg) and M. grandiflora (50 mg/kg) extracts exerted anxiolytic-like effects during the metestrus-diestrus phase, similar to diazepam, without disrupting spontaneous motor activity. No significant effects of the extracts were detected in either behavioral test during the proestrus-estrus phase, whereas diazepam produced motor hypoactivity in the open field test. These results indicate that the M. frutescens and M. grandiflora extracts possess anxiolytic-like effects that depend on the ovarian cycle phase, supporting the Mexican ancient medicinal use of these plants to ameliorate anxiety disorders.

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“…Scopoletin shows MAO inhibition activity in a dose-dependent fashion, with IC 50 values of 19.4 μg/mL [19]. In addition, daphnoretin a bicoumarin of scopoletin and umbelliferone exhibits significant anxiolytic activity in EPM model [21-23]. It has been also suggested that coumarin derivatives interact with the benzodiazepine binding site of the GABA-A receptor [24].…”

Section: Discussionmentioning

confidence: 99%

Coumarin compounds of Biebersteinia multifida roots show potential anxiolytic effects in mice

et al. 2013

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BackgroundTraditional preparations of the root of Biebersteinia multifida DC (Geraniaceae), a native medicinal plant of Irano-Turanian floristic region, have been used for the treatment of phobias as anxiolytic herbal preparation.MethodsWe utilized the phobic behavior of mice in an elevated plus-maze as a model to evaluate the anxiolytic effect of the plant extract and bio-guided fractionation was applied to isolate the active compounds. Total root extract, alkaline and ether fraction were administered to mice at different doses 30 and 90 min prior to the maze test. Saline and diazepam were administered as negative and positive controls, respectively. The time spent in open and closed arms, an index of anxiety behavior and entry time, was measured as an index of animal activity.ResultsThe total root extract exhibited anxiolytic effect which was comparable to diazepam but with longer duration. This sustained effect of the crude extract was sustained for 90 min and was even more after injection of 45 mg/kg while the effect of diazepam had been reduced by 90 min. The anxiolytic effect factor was only present in the alkaline fraction and displayed its effect at lower doses than diazepam while pure vasicinone as the previously known alkaloid did not shown anxiolytic effect. The effect of the alkaline fraction was in a dose dependent manner starting at 0.2 mg/kg with a maximum at 1.0 mg/kg. Bio-guided fractionation using a variety of chromatographic methods led to isolation and purification of three coumarin derivatives from the bioactive fraction, including umbelliferone, scopoletin, and ferulic acid.ConclusionFor the first time, bio-guided fractionation of the root extract of B. multifida indicates significant sustained anxiolytic effects which led to isolation of three coumarin derivatives with well-known potent MAO inhibitory and anti-anxiety effects. These data contribute to evidence-based traditional use of B. multifida root for anxiety disorders.

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The standardized extract of Loeselia mexicana possesses anxiolytic activity through the γ-amino butyric acid mechanism

Cited by 22 publications

References 45 publications

Participation ofGABAAChloride Channels in the Anxiolytic-Like Effects of a Fatty Acid Mixture

Participation ofGABAAChloride Channels in the Anxiolytic-Like Effects of a Fatty Acid Mixture

Montanoa frutescensandMontanoa grandifloraExtracts Reduce Anxiety-Like Behavior during the Metestrus-Diestrus Phase of the Ovarian Cycle in Wistar Rats

Coumarin compounds of Biebersteinia multifida roots show potential anxiolytic effects in mice

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