The State of Water in Muscle Tissue as Determined by Proton Nuclear Magnetic Resonance

Cooke, Roger; Wien, R.

doi:10.1016/s0006-3495(71)86274-4

Cited by 86 publications

(25 citation statements)

References 24 publications

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“…Exposure of fully acclimated crowns to dehardening conditions (20 C) for 7 days resulted in an average loss in cold hardiness of 7 C for the four cultivars and a reduction in AVY2 from 16.7 to 13.9 Hz. When these partially dehardened crowns were again exposed to acclimating conditions (5 C light, 12 h and 3 C dark) for 6 days, their hardiness increased by 4 C and AVY2 increased slightly to 14.4 Hz.…”

Section: Continuous Wave Nmr Measurementsmentioning

confidence: 99%

A Nuclear Magnetic Resonance Study of Water in Cold-acclimating Cereals

et al. 1979

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Continuous wave nuclear magnetic resonance (NMR) studies indicated that the line width of the water absorption peak (AP%) from crowns of winter and spring wheat (Titicum aestivum L.) increased during cold acclimation. There was a negative correlation between Air and crown water content, and both of these parameters were correlated with the lowest survival temperature at which 50% or more of the crowns were not killed by freezing (LT5o). Regression analyses indicated that APS and water content account for similar variability in LT5w Slow dehydration of unacclimated winter wheat crowns by artificial means resulted in similarly correlated changes in water content and A4Y. Rapid dehydration of unacclimated crowns reduced water content but did not influence AP%. The incubation of unacclimated winter wheat crowns in a sucrose medium reduced water content and increased Au's. The increase in AP% appears to be dependent in part on a reduction in water content and an increase in solutes.Longitudinal (TO) and transverse (T2) relaxation times of water protons in cereals at different stages of cold acclimation were measured using pulse NMR methods. The T, and T2 signals each demonstrated the existence of two populations of water, one with a short and one with a long relaxation time. During the first 3 weeks of acclimation, the long T2 decreased significantly in winter-hardy cereals, and did not change in a spring wheat until the 5th week of hardening. There was no change in the long T, until the 3rd week of hardening for the winter cereals and until the 7th week of hardening for the spring wheat. No simple relationship could be established between T, or T2 and cold hardiness. Neither continuous wave or pulsed NMR spectroscopy can be used as a diagnostic tool in predicting the cold hardiness of winter wheats. An increase in AvA or a reduction in relaxation times does not provide evidence for ordering of the bulk of the cell water.water content and an increase in hydrophilic compounds such as sugars (17), proteins (24), and nucleic acids (23). These hydrophilic compounds could affect the physical state of water which in turn would affect the amount of water which crystallizes at any given subzero temperature.Continuous wave NMR studies on animal tissue have shown that line width (Av½, one-half maximum amplitude of the water absorption peak) is significantly broader than in pure water (6,15). This has led to the suggestion that cellular water consists of several populations, including a "bound" or unfreezable fraction (1,5,6 MATERIALS AND METHODS CONTINUOUS WAVE NMR MEASUREMENTSThe physical state of water in plant cells has long been a subject of interest in relation to cold hardiness (16,17,22). NMR3 provides one method of studying water and water interactions in intact biological systems (1,2,6,11,18,19 Samples for analysis were stripped to two leaves and 3-cm sections were cut from just above the base. Studies of the effect of sample shape and spinning rate on line width showed that crooked samples had a line width ...

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Section: Continuous Wave Nmr Measurementsmentioning

confidence: 99%

A Nuclear Magnetic Resonance Study of Water in Cold-acclimating Cereals

et al. 1979

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“…Evidence for osmotically inactive fiber water in muscle has existed for many years? Recently, the question of structured, nonsolvent, or osmotically inactive fiber water has been intensively investigated by a number of techniques including osmotic (Dydynska&Wilkie, 1963;Reuben, Lopez, Brandt&Grundfest, 1963;Bozler, 1965;Hinke, 1970), microelectrode (Hinke, 1970) and NMR measurements (Cope, 1967(Cope, , 1972Hazelwood, Nichols & Chamberlain, 1969;Cooke &Wien, 1971;Chang, Hazelwood, Nichols & Rorsehach, 1972;Civan & Shporer, 1972;Outhred & George, 1973). A full discussion of these results is outside the scope of this paper.…”

Section: State and Distribution Of Fiber Potassiummentioning

confidence: 99%

State and distribution of potassium and sodium ions in frog skeletal muscle

Lee

Armstrong

1974

J. Membrain Biol.

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Summary.A modified method for constructing cation-selective glass membrane microelectrodes is described. The method permits routine fabrication of electrodes with tip diameters less than 1 g and exposed tip lengths of 2 to 5 I1. These electrodes had tip resistances in the range 107 to 109 ohms and gave stable and reproducible potentials in standard NaC1 and KC1 solutions. Potentials were unaffected by pH in the range 6 to 8 and satisfactory calibration curves were obtained over the temperature range 15 to 25 ~ Pairs of electrodes with different K-Na selectivity coefficients were used to measure K + and Na + activities in frog sartorius fibers. In fibers containing normal amounts of these ions, Na + activity was much less than would be predicted from chemical analysis of the muscles, assuming that all the apparent muscle Na + is present in an osmotically active form in the myoplasm. Possible origins of this discrepancy are discussed. Following a 48-hr soak at 5 ~ in a K-free medium, the apparent Na + concentration and activity of the fibers both increased and the K + concentration and activity decreased. Comparison of the changes in fiber K + and Na + activities under these conditions with the corresponding changes in fiber concentrations strongly suggests the existence in normal fibers of at least two intrafiber K + compartments, both of which exchange with external Na + but only one of which is detectible by a microelectrode located in the myoplasm. Ca ++ appears to exert a strong regulatory effect on Na+-K + exchange between these compartments and the external medium and on the distribution of intrafiber Na + and K + between these compartments.At present, much interest centers upon the state of Na § and K + in skeletal muscle fibers and other cells. Many investigations have indicated that the internal Na § of skeletal muscle is not uniformly distributed throughout the apparent fiber water.

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“…[I] and [2], the transient development of M,,(T); where T = w,t, under saturation can be written After the fits, the error estimates of the parameters were evaluated by using standard numerical procedures (31) involving Hessian and correlation matrices.…”

Section: Introductionmentioning

confidence: 99%

Temperature dependence and phase transition of proton relaxation of hydrated collagen in intact beef tendon specimens via cross‐relaxation spectroscopy

Tzou¹,

Lee²,

Yeung³

1997

Magnetic Resonance in Med

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The role of water of hydration in proton relaxation in tissues as exemplified by hydrated collagen in beef tendon was studied as a function of temperature from -40 degrees to 37 degrees C by using cross-relaxation spectroscopy. Experimental data were fitted to a simple binary spin-bath model. The outcome of this procedure allows the construction of a semi-quantitative depiction of proton relaxation in a heterogeneous system and its change as one of the water fractions freezes at about -10 to -20 degrees C, a transition observed by NMR and confirmed independently by differential scanning calorimetry. Such physical depiction provides a crude but insightful interpretation of the role "bound" water plays in proton relaxation. This may be important in shedding light on the mechanism of tissue relaxation and its role in MRI diagnosis, particularly for those diseases such as liver cirrhosis where the water-macromolecular interaction plays a prominent role.

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The State of Water in Muscle Tissue as Determined by Proton Nuclear Magnetic Resonance

Cited by 86 publications

References 24 publications

A Nuclear Magnetic Resonance Study of Water in Cold-acclimating Cereals

A Nuclear Magnetic Resonance Study of Water in Cold-acclimating Cereals

State and distribution of potassium and sodium ions in frog skeletal muscle

Temperature dependence and phase transition of proton relaxation of hydrated collagen in intact beef tendon specimens via cross‐relaxation spectroscopy

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