The status of global DNA methylation in the spermatozoa of smokers and non-smokers

Hamad, Mohammed; Dayyih, Wael Abu; Laqqan, Mohammad; Alkhaled, Y.; Montenarh, Mathias; Hammadeh, Mohamad Eid

doi:10.1016/j.rbmo.2018.08.016

Cited by 27 publications

(27 citation statements)

References 62 publications

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“…Barzideh et al 38 revealed that poor‐quality spermatozoa have globally hypermethylated DNA, which is probably associated with apoptosis and sperm vitality. Furthermore, a study by Hamad et al 39 presented that methylation is higher in samples from smokers and in samples with higher levels of non‐condensed chromatin, which indicates that global sperm DNA methylation levels can be associated with the results of advanced sperm quality parameters and can be influenced by environmental/lifestyle causes. It is already suggested that certain environmental factors can be responsible for specific epigenetic modifications, although it is not clear if they are non‐specific or if they may lead to physiological adjustment 11 .…”

Section: Discussionmentioning

confidence: 99%

“…When interpreting sperm DNA methylation data, the quality of spermatozoa should also be taken into consideration. Studies have shown that global sperm DNA methylation is lower in cases of impaired sperm counts and motility, 37 but there are also studies opposing these data 38,39 . Barzideh et al 38 revealed that poor‐quality spermatozoa have globally hypermethylated DNA, which is probably associated with apoptosis and sperm vitality.…”

Section: Discussionmentioning

confidence: 99%

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Minor DNA methylation changes are observed in spermatozoa prepared using different protocols

Štimpfel

Vrtačnik-Bokal

2020

Andrology

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Background DNA methylation patterns can show transgenerational inheritance and are influenced by lifestyle and environmental factors. It is suggested that these patterns can be changed by assisted reproductive technology. Objectives To evaluate the impact of two different sperm preparation methods, conventional density gradient centrifugation (DGC) vs. density gradient centrifugation followed by magnetic‐activated cell sorting (MACS) of non‐apoptotic spermatozoa, on sperm DNA methylation profile. Materials and methods We analyzed semen of patients included in our IVF treatment program. Half of the semen from each included patient was prepared for ICSI using the DGC method and the other half with DGC followed by MACS. The remaining samples were processed for DNA methylation analysis with reduced representation bisulfite sequencing (RRBS). In addition to the DNA methylation profile, we assessed the morphology and DNA fragmentation of spermatozoa. Results RRBS analysis revealed that the average genome‐wide methylation level was similar between both groups (DGC vs. MACS group) and ranged from 0.53 to 0.56. Furthermore, RRBS analysis identified 99 differentially methylated regions (DMRs) and 800 differentially methylated positions (DMPs). In the DGC group, 43 DMRs and 392 DMPs were hypermethylated whereas 56 DMRs and 408 DMPs were hypomethylated compared with those in the MACS group. When DMRs and DMPs were annotated to genes, 3 genes associated with imprinting were found: IGF2, PRDM16, and CLF4/BRUNOL4. The percentage of morphologically normal spermatozoa (MACS vs. DGC; 14.0 ± 10.8 vs. 13.2 ± 10.0; P = .335) and of spermatozoa with fragmented DNA of patients with RRBS analysis (22.9 ± 21.1% vs. 34.4 ± 21.2; P = .529) were also similar between groups. Discussion and Conclusion Although the average genome‐wide level of sperm DNA methylation was similar in both sample groups, a distinctive number of methylation changes were observed in DMR and DMP levels. A larger number of samples should be analyzed and additional sperm preparation methods should be tested to confirm our findings.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Minor DNA methylation changes are observed in spermatozoa prepared using different protocols

Štimpfel

Vrtačnik-Bokal

2020

Andrology

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“…Interestingly, the assembly of NPBs and the accompanying pronuclear RNA synthesis coincide with the assembly of microtubule organizing centers in human zygotes [43], and abnormalities of NPB assembly are associated with abnormal development of human preimplantation embryos [37,44] and an increased risk of embryo aneuploidy [45]. Some conditions potentially responsible for sperm epigenetic abnormalities, such as advanced paternal age [46], tobacco smoking [47], and various lifestyle factors including dietary habits, physical activity or alcohol consumption [48], have been pointed out. However, a more comprehensive analysis of factors involved in these phenomena remains a big challenge for future research.…”

Section: Fertilization With Abnormal Spermatozoamentioning

confidence: 99%

Paternal Effects on Embryonic, Fetal and Offspring Health: The Role of Epigenetics in the ICSI and ROSI Era

Tesarik

2020

Innovations in Assisted Reproduction Technology

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Paternal effects on the developmental potential of human embryos have been studied since the early 1990s, particularly with respect to newly emerging assisted reproduction technologies. Both genetic and epigenetic paternal effects can influence postfertilization development and cause implantation failure or miscarriage. However, it is only over the last few years that issues related to paternal effects associated with different assisted reproduction techniques on the health status of newborn and adult progeny have been focused. At the same time, new findings point out different, yet unexplored, areas of research into the potentially responsible factors, including the activity of the sperm-derived oocyte-activating factor and the oocyte signaling pathways mediating its action, the methylation status of both imprinted and non-imprinted genes, correct replacement of sperm nuclear protamines with oocyte-derived histones, the histone acetylation status, and the function of sperm-borne small RNAs. It is increasingly important to know how these developmentally important epigenetic regulators can be altered in the context of the current micromanipulation-assisted fertilization techniques, intracytoplasmic sperm injection (ICSI) and round spermatid injection (ROSI). Last but not least, transgenerational transmission of acquired, environmentally conditioned disorders from fathers to offspring is a newly emerging issue which warrants further research.

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“…Among various effects underlined, smoking interferes with DNA methylation that is significantly correlated with sperm parameters while the life of seminal leukocytes is doubtful [1] [2] [3]. Nicotine could damage sperm through nicotinic receptors.…”

Section: Introductionmentioning

confidence: 99%

“…The damage of sperm chromatin condensation and sperm viability depends on the number of cigarettes smoked per day and on the duration of How to cite this paper: Neri, A., Banci, M., Pietropolli, A., Gloria-Bottini, F. and Magrini, A. (2019) Smoking, ACP 1 [5]. Aryanpur, comparing smokers with non-smokers, has shown that active cigarette consumption, even if in small amount, decreases sperm concentration while no significant correlation has been found with morphology or motility [6].…”

Section: Introductionmentioning

confidence: 99%

Smoking, ACP<sub>1</sub> and Infertility in Subjects with Varicocele

Neri¹,

Banci²,

Pietropolli³

et al. 2019

Health

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Several studies suggest that smoking may have detrimental effect on seminal parameters and that genetic polymorphisms may influence this effect. In the present paper we have examined the relationship between ACP 1 genotypes and the association of smoking and infertility in a sample of men with varicocele. 126 subjects with varicocele were studied. ACP 1 genotype was determined by DNA analysis and statistical analyses were performed by SPSS software. In general there is a protective action of low activity *A/*B genotype against infertility that is much more marked in smokers than in non-smokers. If confirmed in other clinical settings, determinations of ACP 1 genotype could have practical importance to evaluate the risk of infertility in smokers with varicocele.

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The status of global DNA methylation in the spermatozoa of smokers and non-smokers

Cited by 27 publications

References 62 publications

Minor DNA methylation changes are observed in spermatozoa prepared using different protocols

Minor DNA methylation changes are observed in spermatozoa prepared using different protocols

Paternal Effects on Embryonic, Fetal and Offspring Health: The Role of Epigenetics in the ICSI and ROSI Era

Smoking, ACP<sub>1</sub> and Infertility in Subjects with Varicocele

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The status of global DNA methylation in the spermatozoa of smokers and non-smokers

Cited by 27 publications

References 62 publications

Minor DNA methylation changes are observed in spermatozoa prepared using different protocols

Minor DNA methylation changes are observed in spermatozoa prepared using different protocols

Paternal Effects on Embryonic, Fetal and Offspring Health: The Role of Epigenetics in the ICSI and ROSI Era

Smoking, ACP&lt;sub&gt;1&lt;/sub&gt; and Infertility in Subjects with Varicocele

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Smoking, ACP<sub>1</sub> and Infertility in Subjects with Varicocele